| Project/Area Number |
10556061
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OTSUKA Haruki Grad. Sch of Agricul. and Life Sci., The University of Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (80261957)
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| Co-Investigator(Kenkyū-buntansha) |
XUAN Xuenan Protozoan Res. Center, Obihiro, University, Associate Professor, 原虫病研究センター, 助教授 (10292096)
MATSUMOTO Yoshitsugu Grad. Sch of Agricul. and Life Sci., The University of Tokyo, Associate Professor, 大学院・農学生命科学研究科, 助教授 (00173922)
MATSUMOTO Yasunobu Grad. Sch of Agricul. and Life Sci., The University of Tokyo, Associate Professor, 大学院・農学生命科学研究科, 助教授 (90251420)
SHIBATA Isao Zennoh Institute for Animal Health, Researcher, 研究開発室, 主任調査員(研究員)
IWATA Akira Nippon Institute for Biological Sciences, Researcher, 研究部, 研究員
小沼 操 北海道大学, 獣医学部, 教授 (70109510)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | Interferon-g / Cytokine / Interleukin-12 / Vaccinia virus / Recombinant virus / Neospora caninum / Cryotosporidium parvum / Tachyzoit / 寄生原虫 / インターフェロン / アポトーシス / IFN-γ / CHV / NcRS2 / IL-12 / p35 / p40 / IRES / ワクチニアウイルス / p7.5プロモータ / tunicamycin / N-linked糖鎖付加 / イヌヘルペスウイルス / INF-γ / LPS |
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| Research Abstract |
1) The canine interferon-γ gene was cloned and integrated into the genome of vaccinia virus. When cells were infected with this recombinant vaccinia virus, the presence of canine interferon-γ with biological activity was confirmed.
2) The canine interleukin-12 gene was cloned and integrated into the genome of vaccinia virus. When cells were infected with this recombinant vaccinia virus, the presence of canine interleukin 12 with biological activities was confirmed.
3) To develop a vaccine against Neospora caninum infection, recombinant vaccinia virus carrying the NcSRS2 and NcSAG1 genes were constructed and tested in a mouse model. Vaccination of dams with the recombinant conferred effective protection against vertical transmission of Neospora caninum to offspring
4) A canine herpesvirus recombinant carrying the NcRS2 gene of Neospora caninum was constructed. Indirect immunofluorescence indicated that the antigenic structure of the recombinant NcSR2 was similar to the authentic parasite protein. Vaccination of dogs with this recombinant canine herpesvirus induced antibodies which reacted with the surface antigen of Neospora caninum
5) Two lines of recombinant vaccinia virus, vv/32C and vv/32I, which carry the p32 genes of the Chitose and Ikeda types of Theileria segenti were constructed. Vv/C32 and vv/I32 produced type-specific p32 which did not cross react with the monoclonal antibodies against the other type of p32. When mice was immunized with these recombinant antibodies against p32 were detectable 2 weeks after the immunization
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