| Project/Area Number |
10556067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Applied veterinary science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SUGIMOTO Chihiro HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF VETERINARY MEDICINE, ASSOCIATE PROFESSOR, 大学院・獣医学研究科, 助教授 (90231373)
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| Co-Investigator(Kenkyū-buntansha) |
UYEDA Ichiro HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF AGRICULTURE, PROFESSOR, 大学院・農学研究科, 教授 (10113523)
OHASHI Kazuhiko HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF VETERINARY MEDICINE, INST., 大学院・獣医学研究科, 助手 (90250498)
ONUMA Masao HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF VETERINARY MEDICINE, PROFESSOR, 大学院・獣医学研究科, 教授 (70109510)
MATSUMURA Takeshi HOKKAIDO GREENBIO INSTITUTE, RESEARCHER, 研究員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 1999: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1998: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | INTERFERON / TRANSGENIC PLANTS / CYTOKINE / PLANT VIRUS VECTOR |
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| Research Abstract |
The aim of this study is to express bioactive animal proteins in plants and to apply the recombinant proteins for animal disease control. We used transgenic plant and plant viral vector stems for expression of animal genes.
By Agrobacterium-mediated transformation, we introduced cDNAs of two subtypes of human interferon α (IFNα2b and IFNα8). Expression of the introduced genes was confirmed by northern blot analysis and enzyme immunoassay. Ten to 500 IU/g of IFN8 were detected in transgenic potatoes.
In bovine leukemia virus infection, tumor necrosis factor α was revealed to be involved in pathogenesis, especially control of viral burden in infected animals. We also clones genes for a tick salivary grand protein, tick serine proteases, and a piroplasm surface antigen that are expected to be expressed in plant and used for method of disease controls.
A plant expression system based on clover yellow vein virus has been developed by combining its cDNA and 35S promoter as pC1YVV. Expression of green fluorescence protein by this virus vector was confirmed. INF8 cDNA was introduced into this vector and its expression was tested. However, plant infected with this recombinant virus did not express any product as tested by enzyme immunoassay, provably because the viral vector had not retained the cDNA. Further investigation will be required for stable expression of animal genes in this system.
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