The discovery of new physiological effect of histamine by gene-manipulated mice and its application for the creation of pathological model
| Project/Area Number |
10557010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General pharmacology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
OHTSU Hiroshi Medicine, Tohoku University, Lecturer, 大学院・医学系研究科, 講師 (60250742)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Noboru Kazusa Research Pharm. Center, Mitsubishi-Tokyo Pharm. Co., Lab. Head, かずさ研究所創薬研究所, 主任研究員
ICHIKAWA Atsushi Pharmacy, Kyoto University, Professor, 大学院・薬学研究科, 教授 (10025695)
WATANABE Takehiko Medicine, Tohoku University, Professor, 大学院・医学系研究科, 教授 (70028356)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1999: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1998: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | histamine / transgenic mouse / histidine decarboxylase / knockout mouse / allergic reaction / behavior / 遺伝子 / レスキュー / 眼電位図 / 行動薬理 |
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| Research Abstract |
1) We elucidated HDC gene knockout mice. They lack histamine from the whole body ; therefore it is useful for the histamine research.
2) We found several interesting phenotypes including behavior, allergic reaction, and inflammation.
3) For instance, knockout mice showed no extravasation reaction, confirmed by the Evan's blue leakage from the vessel in the immediate-type skin reaction. Moreover, this disappearance of skin reaction was "rescued" by orally supplemented histamine. This shows that the extravasation reaction succeeded after immediate type allergic reaction is controlled by histamine.
4) In the systemic anaphylaxis reaction histamine has been proved to be one of the very important mediators.
5) The activity of the mice during the day and night is also controlled with histamine.
6) Some response to infection was different between wild type and knockout type.
7) It will be high possibility that the mice show different response in tumor infiltration and metastasis. We are planning to irradiate ultraviolet rays or inoculate the cultured cancer cells into the mice and observe the infiltrative and metastatic process.
8) We prepared transgenic mice expressing high histamine in the body. We are going to use these mice for the study of histamine effects on the whole body.
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Report
(3 results)
Research Products
(7 results)
