| Project/Area Number |
10557034
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Virology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TAKIGUCHI Masafumi Center for AIDS Research, Kumamoto University Professor, エイズ学研究センター, 教授 (00183450)
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| Co-Investigator(Kenkyū-buntansha) |
MIWA Kiyoshi Central Research Laboratories, Ajinomoto Co., Director, 創薬研究所, 所長
TOMIYAMA Hiroko Center for AIDS Research, Kumamoto University Assistant Professor, エイズ学研究センター, 助手 (50301370)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | HLA class I / cytotoxic T lymphocyte / epitope / vaccine / HLA-A11 / HIV-1 / reverse immunogenetics / HLA-A33 / 細胞傷害性T細胞 |
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| Research Abstract |
In the present study, we identified multiple HIV-1, HCV and HBV-specific cytotoxic T cell epitopes using reverse immunogengetics.
(1) Identification of HLA-A^* 1101-restricted HIV-1 CTL epitopes : We identified three and one CTL epitopes derived from Pol and Env proteins of HIV-1 clade B, respectively. Furthermore, we investigated whether 8 clade B CTL epitopes including the four epitopes are also clade E epitopes. Three were cross-clade epitopes between clade B and E while three clade E-specific sequences corresponding to clade B epitopes were clade E-specific epitopes.
(2) Identification of HIV-1 clade B epitopes presented by HLA-A^*3303 : We identified 6 HIV-1 clade B epitopes presented by HLA-A^*3303 using reverse immunogenetics.
(3) Identification of HLA-A^*2402-restricted HBV-specific CTL epitopes : We identified two HLA-A^*2402-restricted HBV-specific CTL epitopes using reverse immunogenetics. These epitope-specific CTL were detected in acute phase PBMC from individuals infected with acute HBV hepatitis by specific tetramers.
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