| Project/Area Number |
10557049
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
内科学一般
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MORIMOTO Chikao Institute of Medical Science, The University of Tokyo, Professor, 医科学研究所, 教授 (30119028)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Hiroshi Institute of Medical Science, The University of Tokyo, Assistant Professor, 医科学研究所, 助手 (80280957)
HOSONO Osamu Institute of Medical Science, The University of Tokyo, Assistant Professor, 医科学研究所, 助手 (50190210)
TANAKA Hirotoshi Institute of Medical Science, The University of Tokyo, Associates Professor, 医科学研究所, 助教授 (00171794)
TANAKA Toshiaki Toray Industries, Inc., Group Leader (researcher), 基礎研究所, 主任研究員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | CD26 / dipeptidyl peptidase IV / adenosine deaminase / T cell costimulation / mannose 6-phosphate / insulin-like growth factor II receptor / インスリン成長因子II受容体 / RANTES / MIP-1β / SDF-1α / リンパ球遊走 / HIV感染 |
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| Research Abstract |
CD26 is the T cell activation antigen and also known to bind the adenosine deaminase (ADA) and have a dipeptidyl peptidase IV (DPPIV) activity. Cross-linking of CD26 and CD3 with immobilized monoclonal antibodies can deliver a second costimulatory signal and contribute to T cell activation. Our earlier studies revealed that the cross-linking of CD26 induced internalization of the molecules, subsequent tyrosine phosphorylation of signaling molecules and resulted in an enhanced proliferating responses of T cells. Although these findings suggests the importance of internalization in the function of CD26. CD26 has only 6 amino acid residues in its cytoplasmic region with no known motif for endocytosis. In this study, we have identified the mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGFIIR) as the binding protein for CD26, and the mannose 6-phosphate (M6P) residues in carbohydrate moiety of CD26 is critical for the binding between CD26 and M6P/IGFIIR. In human peripheral blood lymphocytes, T cell activation resulted in the mannose 6-phosphorylation of CD26. In addition, the cross-linking of CD26 with an anti-CD26 antibody induced not only capping and internalization of CD26 but also colocalization of CD26 with M6P/IGFIIR. Finally, internalization of CD26 by cross-linking and T cell proliferation induced by CD26 mediated T cell costimulation were inhibited by the addition of M6P, but not by the glucose 6-phosophate or mannose 1-phosphate. These results indicate that the interaction between mannose 6-phosphorylated CD26 and M6P/IGFIIR may play an important role in CD26-mediated T cell constimulatory signaling.
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