| Project/Area Number |
10557101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Endocrinology
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| Research Institution | Osaka University |
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Principal Investigator |
MATSUZAWA Yuji Graduate School of Medicine, Osaka University, Professor, 医学系研究科, 教授 (70116101)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Tadashi Graduate School of Medicine, Osaka University, Assistant Professor, 医学系研究科, 助手 (90252668)
FUNAHASHI Tohru Graduate School of Medicine, Osaka University, Assistant Professor, 医学系研究科, 助手 (60243234)
YAMASHITA Shizuya Graduate School of Medicine, Osaka University, Lecturer, 医学系研究科, 講師 (60243242)
NAKAZAWA Hiroshi Biotechnical laboratory, Sumitomo chemical company, Ltd. Research Director, 生命工学研究所, 研究員
KIHARA Shinji Osaka University Hospital, Medical Stuff, 医学部・附属病院, 医員
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1999: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1998: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | obesity / visceral fat / life style-related diseases / atherosclerosis |
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| Research Abstract |
Accumulation of excess body fat has become a common basis for the development of non-insulin dependent diabetes mellitus (NIDDM) and atherosclerotic vascular diseases (AVD). Body fat distribution is a independent r the risk for above diseases. Deposition of intra-abdominal viscreal fat rather than subcutaneous fat closely relates to the development of NIDDM and AVD. The aim of this study is to clarify the biological difference between subcutaneous and visceral fat tissues and to apply the data to clinical medicine.
By the comparison of the gene expression profiles of subcutaneous and visceral fat tissues, subcutaneous fat has been revealed predominantly to express "static" genes for the proteins maintaining fundamental cellular functions including ribosomal protein and cytoskeleton proteins. On the other hand, visceral fat predominantly expressed the genes for a variety of secretory proteins with biological activities.
We focused on two genes, that is, adiponectin, which was obtained by
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a large-scale-random sequencing of adipose-tissue cDNA library and clone 085, which was isolated by diferential display technique. Adiponectin is plasma protein specifically produced and secreted from adipose tisuue. Plasma levels of adiponectin, however, decreased in the obese subjects, especially those with visceral fat accumulation. Marked reduction of plasma adiponectin concentration was observed in he patients with coronary artey disease compaired with BMI-matched conrols. Therefore, decreased plasma adponectin has been proven to be a potential risk for coronary artery disease. The proein showed anti-atherogenic properties, including suppresion of monocyte-adhesion to endothelial cells and proliferation of vascular diseases.
The product of clone 085 belonged to hematopoietic factors The expression of mRNA for clone 085 increased in visceral fat during the development of obesity. Plasma levels of clone 085 products increased in paralel with the amount of visceral fat in mice. In humans, plasma 085 level was closely correlated with the amount of visceral fat but not with subcutaneous fat.
In this study, we investigated in the visceral fat derived factors. The measurement of plasma levels of these visceral fat derived factors will be a clue for understanding the pathogenesis of diseases accompanied by visceral fat obesity. Less
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