| Project/Area Number |
10557106
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General surgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SUGIURA Hiroshi Hokkaido Univ., Medical Hospital, Assis. Pro., 医学部・附属病院, 助手 (30292022)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Satoshi Chiba Univ., Fac. Of Med., Pro., 医学部, 助教授 (30215454)
ISHIKURA Hiroshi Hokkaido Univ., Fac. Of Med., Pro., 医学部, 教授 (70222982)
KATOH Hiroyuki Hokkaido Univ., Medical Hospital., Assis. Pro., 医学部, 教授 (80002369)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥9,100,000 (Direct Cost: ¥9,100,000)
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| Keywords | Pancreatic Cancer / IL-6 / Gene Therapy / Immunogene Therapy |
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| Research Abstract |
Production of interleukin-6 (IL-6) by human pancreatic carcinoma cells inversely correlates with potentials for blood-borne metastasis to the liver in nude mice. IL-6 cDNA was transfected to PCI-43, one of our cultured pancreatic carcinoma cell lines that does not produce IL-6 and generates numerous metastases to the liver. An IL-6 high-producer clone (PCI-43h) generated few metastases; IL-6 production thus has a direct effect on metastasis, whereas other transfectants (PCI-43l and PCI-43n), which are IL-6 low-, and IL-6 non-producers, respectively, did generate metastases. Tumor-reactive IgG, which mediated antibody-dependent cellular cytotoxicity (ADCC) in vitro, was detected in sera from recipient nude mice inoculated with PCI-43h but not in sera from mice given PCI-43l, PCI-43n or parent PCI-43. In addition, we tested the efficacy of N116Y in blocking the in vivo growth of pancreatic cancer cells using an adenoviral vector. Direct injection of the AdCEA-N116Y in which N116Y expression is driven by the CEA promoter, into the spleen in nude mice suppressed their growth of liver metastases significantly.
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