| Project/Area Number |
10557108
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General surgery
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
DOI Hideyuki Tohoku University Hospital, Lecturer, 医学部・附属病院, 講師 (90188839)
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| Co-Investigator(Kenkyū-buntansha) |
ORI Takashi Tohoku University Hospital, Research Associate, 医学部・附属病院, 助手 (20282048)
SHINEHA Ryuzauro Tohoku University Hospital, Lecturer, 医学部・附属病院, 講師 (20192106)
OHKOHCHI Nobuhiro Graduate Sch. of Medicine, Tohoku Univ., Associate Prof., 大学院・医学系研究科, 助教授 (40213673)
CHUBACHI Seiji Tohoku University Hospital, Research Associate, 医学部・附属病院, 助手 (90302108)
SEKIGUCHI Satoshi Tohoku University Hospital, Research Associate, 医学部・附属病院, 助手 (20312580)
桜田 正寿 東北大学, 医学部・附属病院, 助手 (40292320)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1998: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | macrophage / kupffer cell / Liver transplantation / dichloromethylene bisphosphonate / rejection / クッパー細胞(Kc) / リポソーム包埋クロドロネート / LE-Cl_2MDP |
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| Research Abstract |
Background and Aim. We examined the roles of kupffer cells and macrophages in the prolongation of graft survival in allogeneic rat liver transplantation.
Materials and Methods. This study consisted of 5 experimental groups of rats underwent allogeneic liver grafts. The rats in Group I and V were transplanted with non-treated grafts, rats in Group V were administered with liposome encapsulated dichloromethylene bisphosphonate intravenously six times after liver transplantation. The rats in Group II were transplanted with kupffer cells-depleted grafts. The rats in Group III or IV were transplanted with non-treated grafts or kupffer cells-depleted grafts respectively, and both groups received twice injections of liposome encapsulated dichloromethylene diphosphonate after liver transplantation. The animals were examined daily to determine the survival period. The rats were killed on days 3, 6, 9, 12, 15, 21 and 30. The liver grafts were examined taking into account the liver histopathology and immunohistochemistry.
Results. In Group IV, 60% of the grafts survived for >150 days. The graft survival periods were significantly longer than those of other groups. Adoptive transfer of serum of long survived rat did not affect the heart graft survival in rat, which underwent heart transplantation without any treatments. The long-survived rats of Group IV did not reject the second graft of allogeneic skin. Although the level of total serum bilirubin, the number of mononuclear cells and ED2 positive cells of grafts increased gradually until two weeks after liver transplantation in all group, the number of ED2 positive cells began to decrease on postoperative day 12 in Group IV. Histologically, the decrease of ED2 positive cells tended to be located around the periportal area.
Conclusions. These findings suggested that the prolongation of liver graft survival could be result from the depletion of kupffer cells in liver graft and recipient macrophage migrating to the graft.
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