| Project/Area Number |
10557112
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
General surgery
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| Research Institution | Kobe University |
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Principal Investigator |
KURODA Yoshikazu Kobe University school of Medicine, Professor, 医学部, 教授 (70178143)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yasuyuki Kobe University school of Medicine, Research Associate, 医学部, 助手 (40304092)
KU Yonson Kobe University school of Medicine, Professor, 医学部, 助教授 (40195615)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1998: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Pancreas transplantation / Warm ischemic injury / Two-layer method / UW simple cold storage / Protein synthesis / Stress protein |
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| Research Abstract |
1. Based on our previous canine experiments, pancreas grafts subjected to 90 min warm ischemia, which cannot survive after implantation with no treatment, could be successfully transplanted after 5-hour preservation by the two-layer method. On the hypothesis that protein synthesis during the two-layer preservation would be associated with this graft resuscitation, the following experiments were undertaken. We preserved canine pancreases damaged by 90-min warm ischemia using the two-layer method (perfluorochemical/UW solution) for 5 hrs at 20℃. Intragraft DNA, RNA and protein synthesis were quantitated durins preservation by determining incorporation of tritiated thymidine, tritiated uridine and tritiated leucine, respctively, added in this preservation solution. Graft viability was judged from graft survival after autotransplantation in the cervical subcutaneous cavity. None of 7 grafts (0%) survived when they were immediately transplanted after warm ischemia, while all of 7 grafts (100%) transplanted after the two-layer preservation for 5 hrs at 20℃ survived. Significant increases in RNA and subsequent protein syntheses were observed during preservation by the two-layer method, not by UW simple cold storage. In contrast, DNA synthesis did not follow RNA and protein synthesis. We concluded that protein synthesis might be associated with the process of postischemic cellular recovery of the pancreas graft during mild hypothermic preservation by the two-layer method.
2. Some reports have indicated that heat shock proteins (HSPs) have important functions in cellular recovery. We studied the role of HSPs in reducing ischemia-reperfusion injury during preservation by the two-layer method. From the results of immunohistochemical examinations and western-blotting of HSPs, HSP-60 was likely associated with functional recovery of warm ischemically damaged pancreas by the two-layer method.
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