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Development of hybrid-kidney for drug intoxication and its clinical application

Research Project

Project/Area Number 10557114
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General surgery
Research InstitutionJichi Medical School

Principal Investigator

TSURUOKA Shuchi  Jichi Medical school, Clinical Pharmacology, Instructor, 医学部, 助手 (50285798)

Co-Investigator(Kenkyū-buntansha) ASANO Yasushi  Jichi Medical School, Nephrology, Professor, 医学部, 教授 (00050500)
SUGIMOTO Kohichi  Jichi Medical School, Clinical Pharmacology, Assistant Professor, 医学部, 講師 (90244491)
FUJIMURA Akio  Jichi Medical School, Clinical Pharmacology, Professor, 医学部, 教授 (90156901)
MUTO Shigeaki  Jichi Medical School, Nephrology, Assistant Professor, 医学部, 講師 (40190855)
Project Period (FY) 1998 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsHybrid-kidney / digoxin intoxication / hemoperfusion / Multidrug reisistance protein / ハイブリット型腎臓 / 血清浄化法 / ホロファイバー型モジュール / ジゴキシン / ドキソルビシン / 薬物中毒 / ホロファイバーモジュール
Research Abstract

In case of drug intoxication, systems for selective, efficient and safe drug removal from body is necessary, however hemoperfusion, a therapy used in clinical medicine at present time, is not enough, For this purpose, we newly developed "hybrid artificial kidney" in which multidrug resistance protein (MDR)-overexpressed renal cells were cultured on a hollow-fiber module. This device selectively removed substrates of MDR (digoxin, doxorubicin) in vitro as well as from circulated blood in animal model of digoxin intoxication. The serum drug concentration in dog was decreased from about 3 to 1 ng/ml after 90 min. treatment and its clearance is about 22 mL/min. The system is much useful and safer than direct hemoperfusion with charcoal. This system represents high biocompatibility especially when we used renal cells obtained from human. This approach is expected to be employed in the future for the treatment of patients with drug intoxication.

Report

(5 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Tsuruoka S. et al.: "Removal of digoxin and doxorubicin by MDR-overexpressing cell cultured in hollow fiber"Kidney Int.. 56. 154-163 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鶴岡秀一, 鈴木誠, 藤村昭夫: "ハイブリッド人工腎臓。-新しい薬物除去法の開発-"人工臓器. 27. 761-764 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka, S., et al.: "Removal of digoxin by column for specific adsorption of B2-microblobulin, -a potential use for digoxin intoxication-"Clin Pharmacol Ther. 69. 422-430 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka, S., et al.: "Cocultured hepatic and renal cell lines provides biohemofiltration with an active transportsystem and metabolism"J. Artif. Organ.. 4. 336-341 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S.: "Secretion of xenobiotics via multidrug resistance protein (MDR) in kidney -application to development of artificial "hybrid-kidney""Clin. Exp. Nephrol.. 5. 137-143 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 鶴岡秀一: "ハイブリッド型人工腎の開発"Pharma Medica. 19. 67-70 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S, et al.: "Vitamin B-bonded hemodialyzer improves neutrophil function and oxidative stress in patients with end-stage renal failure"Am. J. Kidney Dis.. 39. 127-133 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S., K. et al.: "Removal of digoxin and doxorubicin by MDR-overexpressing cell cultured in hollow fiber"Kidney Int.. 56. 154-163 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S., et al.: "Hybrid-kidney for drug removal (in Japanese)"Jpn J Artif Organ.. 27. 761-764 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S., et al.: "Removal of digoxin by column for spedfic adsorption of B2-microblobulin, -a potential use for digoxin intoxication-"Clin Pharmacol Ther.. 69. 422-30 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S., et al.: "Cocultured hepatic and renal cell lines provides biohemofiltration with an active transportsystem and metabolism"J. Artif. Organ.. 4. 336-341 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S.: "Secretion of xenobiotics via multidrug resistance protein (MDR) in kidney -application to development of artificial "hybrid-kidney"-"Clin. Exp. Nephrol. 5. 137-143 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S.: "Development of hybrid-kidney (in Japanese)"Pharma Medica. 19. 67-70 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka S. et al.: "Vitamin E-bonded hemodialyzer improves neutrophil function and oxidative stress in patients with end-stage renal failure"Am. J. Kidney Dis.. 39. 127-133 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuruoka, S et al.: "Removal of digoxin by column for specific adsorption of B2-microblobulin, -a potential use for digoxin intoxication-"Clin.Pharmacol.Ther.. 69. 422-430 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuruoka, S.: "Secretion of xenobiotics via multidrug resistance protein in kidney : applicationto development of artificial "hybrid kidney""Clin Exp Nephrol.. 5. 137-143 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 鶴岡秀一: "ハイブリット型人口腎の開発"Pharma Media. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuruoka, S et al.: "Vitamin E-Bonded Hemodialyzer Improves Neutrophil Function and Oxidative Stress in Patients With End-Stage Renal Failure"Am.J.Kidney.Dis.. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuruoka,S. et al.: "Removal of digoxin by for specific adsorption of B2-microbolbulin,-a potential use for digoxin intoxication-."Clin,Pharmacol,Ther.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 鶴岡秀一: "ハイブリッド人工腎臓。新しい薬物除去法の開発"人工臓器. 27(6). 761-764 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuruoka, S.: "Removal of digoxin and doxorubicin by MDR-overexpressing cell cultured in hollow fiber"Kidney Int.. 56(2). 154-163 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuruoka S: "Removal of digoxin and doxorubicin by MDR-overexpressing cell cultured in hollow fiber." Kidney Int.in press. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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