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Identification of genes associated with epithelial growth using Ileojejunal transposition model

Research Project

Project/Area Number 10557118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Digestive surgery
Research InstitutionTohoku University

Principal Investigator

FUKUSHIMA Kouhei  Tohoku University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20271900)

Co-Investigator(Kenkyū-buntansha) SASAKI Iwao  Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (60125557)
NAITO Hiroo  Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (90180223)
FUNAYAMA Yuji  Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (50192315)
佐々木 一幸  日清製粉, 創薬研究所, 研究員
笹野 公伸  東北大学, 大学院・医学系研究科, 教授 (50187142)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1998: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsShort boroel Syndrome / total colectomy / molecular biology / epithelial cell / Cell pretiferation / 短腸症侯群 / 細胞分化 / 増殖
Research Abstract

(1)Epithelial gene screening by differential display
a.Ileojeiunal transposition (IJT) model
Ileojejunal transposition was performed using SD rats and samall intestinal epithelial cells were isolated. We subcloned 8 gene and sequenced, resulting that all these clones encoded mitochondrial genes. Differential expresssion was not confirmed, indicating that selection of mitochondrial genes may be due to technical issues accompanied with differential display.
b.Short bowel model
Shor bowel model was established in SD rats and samall intestinal epithelial cells were isolated. We subcloned 11 genes including mitochondrial genes and solute carrier family 9, isoform 3 regulator 1from 80 differential displays.
(2)ornithine decarboxylase(ODC) induction in vitro
IEC6 cells were stimulated 0, 25, 50, and 100 ng/ml glucagon-like peptide 2 (GLP2) and expression of ODC mRNA was examined. Expression of ODC mRNA was stabe after stimulation. In contast, epidermal growth factor, used as a positive control, induced ODC mRNA.
(3)Intestinal adaptation following total colectomy
Rat total volectomy model was established and gene expression of remnant ileal epithelia was analyzed. 11b-hydroxysteroi dehydrogenase type2 (11b-HSD2) was remarkablly induced in the distal ileum probablly due to enhanced aldosterone in blood.
(4)Conclusion
Difficulties in this project was due to 1.inconsistent results from ileojejunal transposition model and 2.limitation of gene screening technique, differential display.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (11 results)

All 2000 1999 Other

All Journal Article (6 results) Publications (5 results)

  • [Journal Article] Urocorin and Corticotropin-relasing factor receptor expression in the human colonic mucosa.2000

    • Author(s)
      村松康成
    • Journal Title

      Peptides 21

      Pages: 1799-1809

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Urocortin and corticotropin-releasing factor receptor expression in the human colonic mucosa2000

    • Author(s)
      Y.Muramatsu, K.Fukushima, et al.
    • Journal Title

      Peptides 21

      Pages: 1799-1809

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Induction of minerale corticoid receptor by sodium butyrate in small intespinal(IEC6) and colonic(T84) epithelial cell lines.1999

    • Author(s)
      福島浩平
    • Journal Title

      Digestive Diseases and Sciences 44

      Pages: 1571-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Gene induction of 11β-hydroxy steroid typeII(11β-HSD2) is essential for enhancednent of aldosttrone-induced sodium absorption1999

    • Author(s)
      佐藤 俊
    • Journal Title

      Gastroenterology 116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Induction of mineralocorticoid receptor by sodium butyrate in small intestinal (IEC6) and colonic (T84) epithelial cell lines1999

    • Author(s)
      K.Fukushima et al.
    • Journal Title

      Digestive Diseases and Sciences 44

      Pages: 1571-1578

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Gene induction of 11β-hydroxysteroid dehydrogenase type II (^<41>β-HSD2) is essential for enhancement of aldosterone-mediated sodium absorption after total proctocolectomy in rats.1999

    • Author(s)
      S.Sato, K.Fukushima et al.
    • Journal Title

      Gastroenterology 116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] A.Hashimoto,K.Fukushima et al.: "Glucagon like peptide-2(GLP-2) neither glicentin (GLI) nor GlP_lenhanced ornithine decarboxylase (ODC) activity"gastroenterology. 118・4 Supple 2. N514 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 福島 浩平: "Induction of mineralocorticoid recepfor by sodium butymate in small intestinal (IEC6) and colonic (T84) epithelial cell lines"Digestive Disease and Sciences. 44. 1571-1578 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 佐藤 俊: "gene induction of 11β-hydroxysteroid type II (11β-HSD2) is essential for enhancement of aldostenone-mediated sodium absorption"gastroenterology. 116. A-1349 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 福島 浩平: "Induction of mineralocorticoid recentor by sodium buty rate in small intestinal(1EC6)and colonic(T84)erithelial cell bines" Digestive Diseases and Sciences. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 児山 香: "Induction of Epithelial Na+ channel in rat ileum after total Colectomy" American Journal of Physiology. (in press).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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