• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ESTABLISHMENT OF NEW IMMUNOSUPPRESSIVE STRATEGY USING GENE TRANSFECTION TECHNIQUE

Research Project

Project/Area Number 10557124
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Thoracic surgery
Research InstitutionOSAKA UNIVERSITY

Principal Investigator

FUKUSHIMA Norihide  Osaka Univ Graduate School of Med Assisstant Professor, 医学系研究科, 助手 (30263247)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Motonobu  Osaka Univ, Graduate School of Med, Assisstant Professor, 医学系研究科, 助手 (90291442)
SAWA Yoshiki  Osaka Univ, Graduate School of Med, Associate Professor, 医学系研究科, 講師 (00243220)
SHIRAKURA Ryota  Osaka Univ, Graduate School of Med, Professor, 医学系研究科, 教授 (00116047)
SAKAKIDA Satoru  Osaka Univ, Graduate School of Med, Assisstant Professor, 医学系研究科, 助手 (90311753)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1998: ¥6,700,000 (Direct Cost: ¥6,700,000)
KeywordsGENE TRANSFECTION / HAMSTER / RAT / HEART TRANSPLANTATION / HJV-LIPOSOME METHOD / GRAFT CORNARY ATHEROSCRELOSIS / BCL-2 / 移植後冠動脈硬化症 / 異種移植 / 遺伝子導入 / HVJリポゾーム法 / アポトーシス / 血管内皮細胞
Research Abstract

It has been reported that endothelial cell activation and apoptosis induced by xenoantibody may play a major role in concordant xenograft rejection as well as direct cell destruction by xenoantibody. Thus, prevention of endothelial cellular apoptosis may prevent graft coronary atheroscrelosis (GCAS). In the present study, the effects of gene transfection of cytoprotective gene, Bcl-2 in graft survival and GCAS was investigated in a hamster-to-rat cardiac transplant (HTx) model in which the recipients were treated with FK506 and cobra venom factor (CVF) to block complement and cellular type rejection.
【Material and Method】 14 Golden Hamster hearts were transplanted into Lewis rat abdomen using Ono-Lindsey methods. All rats were given CVF (0.2 mg/Kg ; day 0 and 1) and FK506 (1 mg/Kg from day 0) intramuscularly. These hearts were divided into two groups. In Group-B (+) and -B (-), grafts were transfected by perfusing coronary artery with HVJ-liposome containing vector with Bcl-2 gene and only vector, respectively. Rejection was defined by cessation of palpation from outside. The explanted grafts were evaluated by H-E staining and immunohistochemical staining of Bcl-2.
【Results】 Graft survival was not significantly different in these groups. Bcl-2 was expressed only in the graft of Group-B (+) within 3 weeks after HTx. Although GCAS score in Group-B (-) was significantly higher than that in Group-B (+), there was no difference between those more than 4 weeks after HTx.
【Conclusion】 Although there was no significant difference in graft survival between both groups, these data suggested that gene transfection of Bcl-2 may prevent endothelial cell activation resulting in preventing graft coronary astheroscrelosis in concordant xenografts.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] K, Svizuki: "Myocardial protection with erdoghoto overeaxpaetsion of marfanece Aceperoxide mutase"Ann Thorac Surg. 68(4). 1266-71 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hilleda: "Gene transfection of hepatoagte giousth factor atlenuates reperfusion injury inthe heart"Ann Thorac Surg. 67(6). 1726-31 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N, Kawahira: "Gene transfection of beta-2-adrenergic receptor into the normal heart enhances cardiac respono to β-agsruist"J, Thorac Cardionasc Surg. 118(3). 446-51 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T, Ohata: "Hy brid artigcial uing with IL-10 aof endothelial corshitlo No syufleface gene-transfection endothelial cells"Circulation. 98(19). II269-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Izutani, S.Miyagawa, R.Shirakura, S.Mikata, M.Tanemura, N.Fukushima, S.Nakata and H.Matsuda.: "Effect of recipient T cell subset depletion on graft coronary arteriosclerosis induction in the rat retransplant model."Transplant Proc. 28. 1828-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Izutani, S.Miyagawa, R.Shirakura, M.Tanemura, S.Mikata, S.K-Sakakida, N.Fukushima, S.Nakata and H.Matsuda.: "Recipient macrophage depletion reduces the severity of graft coronary arterioscrelosis in the rat retransplantation model."Transplant Proc. 29. 861-2 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Sawa, R Morishita, K Suzuki, K Kagisaki, Y Kaneda, K Maeda, K Kadoba, H Matsuda.: "A novel strategy for myocardial protection using in vivo transfection of cis element 'Decoy' against NFkB binding site."Circulation. 96(Suppl). 280-5 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y Sawa, K Kadoba, K Suzuki, H Bai, Y Kaneda, R Shirakura, H Matsuda.: "Efficient gene transfer method into the whole heart through the coronary artery with hemagglutinating virus of Japan liposome."J Thorac Cardiovasc Surg. 113. 512-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K Suzuki, Y Sawa, H Ichikawa, Y Kaneda, H Matsuda.: "Myocardial protection with endogenous overexpression of manganese superoxide dismutase."Ann-Thorac-Surg.. 68(4). 1266-71 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H Ueda, Y Sawa, K Matsumoto, S Kitagawa-Sakakida, Y Kawahira, T Nakamura, Y Kaneda, H Matsuda.: "Gene transfection of hepatocyte growth factor attenuates reperfusion injury in the heart."Ann-Thorac-Surg.. 67(6). 1726-31 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y Kawahira, Y Sawa, M Nishimura, S Sakakida, H Ueda, Y Kaneda, H Matsuda.: "Gene transfection of beta 2-adrenergic receptor into the normal rat heart enhances cardiac response to beta-adrenergic agonist."J-Thorac-Cardiovasc-Surg.. 118(3). 446-51 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T Ohata, Y Sawa, M Takagi, T Inoue, T Yoshida, S Kogaki, H Matsuda.: "Hybrid artificial lung with interleukin-10 and endothelial constitutive nitric oxide synthase gene-transfected endothelial cells attenuates inflammatory reactions induced by cardiopulmonary bypass."Circulation.. 98(19 Suppl). II269-74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y Kawahira, Y Sawa, M Nishimura, S Sakakida, H Ueda, Y Kaneda, H Matsuda.: "In vivo transfer of a beta 2-adrenergic receptor gene into the pressure-overloaded rat heart enhances cardiac response to beta-adrenergic agonist."Circulation.. 98(19 Suppl)(discussion). II262-7-II267-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Suzuki: "Myocardial protection with endogenous overexpression of magnase superoxide mutase"Ann Thorac Surg. 68(4). 1266-1271 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H. Ueda: "Gene transfection of hepatic growth factor alternates reperfusion injury in the heart"Ann Thorac Surg. 67(6). 1726-1731 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi