| Co-Investigator(Kenkyū-buntansha) |
YABUKI Shoji Fukushima Medical College, Dept. of Orthop. Surg., Assistant Prof., 医学部, 助手 (00260779)
KONNNO Shinichi Fukushima Medical College, Dept. of Orthop. Surg., Assistant Prof., 医学部, 講師 (70254018)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1999: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Research Abstract |
Study Design. To study the neural mechanism underlying low back pain and sciatica, distribution of c-Fos protein-like immunoreactivity (fos-IR) in the dorsal horns of spinal cord was examined in adults urethane-anesthetized rats at different survival times after hemiation of intervertebral disc.
Objectives. To analyze the time related changes in c-fos expression after application of nucleus pulposuson a lumbar nerve root using immnohistochemistry.
Summary of Background Data. The hemiated nucleus pulposus produced morphological and functional changes in the adjacent nerve roots (Olmarker et al., 1994; Kayamaet al., 1996). Since these changes were induced without mechanical compression of nerve root, it seems that somewhat chemical factors in the hemiated proposus may produce severe pain and autonomic symptoms.
Methods. Wistar rats (200g, n=79) were anesthetized with urethane pulus alpha-chrolarose and divided into 2 groups. In one group, the L5/6 intervertebral annuls fibrosus of animals w
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ere incised with needles and nucleus pulposus was hemiated on the adjacent 5th lumber nerve roots. In the second group, the dura mater adjust on the L5/6 intervertebral disc was retracted to medial side for 10 seconds and then replaced. Animals were perfused at various times after hemiation, and their distribution of Fos-labelled cells in the L5 were compared between the hemiated and control animal group.
Results. Numbers of Fos-labeled cells in the I and II layers of the dorsal horn in the hemiated group increased to five folds of control animals at 2, 8, 12 and 25 h after hemiation, respectively, while those of in the III and IV layers of the spinal cord increased remarkably at 7, 14 and 28 days after hemiation.
Conclusions. Results indicated that the simple hemiation of nucleus pulposus induced the remarkable increase of fos-IR cells in the dorsal horn, hemiation of nucleus pulposus induced the remarkable increase of fos-IR cells in the dorsal horn, suggesting that hemiated pulposus can induce the induction of genes without mechanical compression of dorsal roots. Less
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