| Project/Area Number |
10557135
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B).
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | TOKAI UNIVERSITY |
|---|
Principal Investigator |
MOCHIDA Joji Tokai University School of medicine, Associate Professor, 医学部, 助教授 (50174347)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKUMA Masahiko Tokai University School of medicine, Assistant Research, 医学部, 助手 (10317787)
西村 和博 東海大学, 医学部, 助手 (20266422)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
|
|---|
| Keywords | disc degeneration / reinsertion of nucleus pulposus / allograft / 髄核 / 再挿入術 / DNA活性 / 再挿入 |
|---|
| Research Abstract |
The purpose of this study is to evaluate effect of allografted disc material for degenerated disc. Experimental animal model for disc degeneration was made using Japanese white rabbits and allogenetic nucleus pulposus or nucleus pulposus cells were reinserted and immunohistochemical reaction was observed. Then, nucleus pulposus itself, 5000 cells or 50000 cells of prepared nucleus pulposus were reinserted into the degenerated discs. Pathological appearance or emergence of type II collagen was studied. No immunological rejection was determined using antirabbit CD4 monodonal antibody or antirabbit CD5 monocional antibody. Reinsertion of allogenetic nucleus pulposus itself was effective to retard further disc degeneration. However, an outcome of the reinsertion using allogenetic nucleus pulpous cells was slightly inferior to the results by nucleus pulposus itself. An increase of chondocyte-like cells in cluster at the inner layer of anulus fibrosus was tightly related to an emergence of type II collagen. On a basis of these results obtained by experimental animal models, an application of coculture of both nucleus pulposus cells and anulus fibrosus cells in human and clinical application under strict set-up were performed. However, reports on those studies using human disc material will be postponed because we are waiting for publication of the peer reviewed journal and arrange to obtain patent on an invention. In 1 year, we are able to report the outcome of our study wholly.
|
