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Preimplantation genetic diagnosis for X-linked genetic diseases and fetal angiographic analysis for fetal aromaties

Research Project

Project/Area Number 10557149
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

YOSHIMURA Yashimura  Keio University School of Medicine, Dept of OB/GYN, Professor, 医学部, 教授 (10129736)

Co-Investigator(Kenkyū-buntansha) ISHIMOTO Hitoshi  Keio University School of Medicine, Dept of OB/GYN, Instructor, 医学部, 助手 (10212937)
MIYAZAKI Toyohiko  Keio University School of Medicine, Dept of OB/GYN, Assistant Professor, 医学部, 講師 (20174162)
SUEOKA Kou  Keio University School of Medicine, Dept of OB/GYN, Associate Professor, 医学部, 助教授 (90162833)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥9,100,000 (Direct Cost: ¥9,100,000)
Keywordspreimplantation genetic diagnosis / Duchenne muscular dystrophy / fetal angiography / Dystrophin / power Doppler
Research Abstract

In preimplantation genetic diagnosis (PGD) for X-linked genetic diseases, gender determination has been used to have clinically unaffected female baby. However, couples undergoing PGD by gender determination can not have boys by the selection procedure. Half of unaffected male embryos must be abandoned. The highly efficient diagnostic technologies is desired to develop for reaching the mutated genes instead of gender determination. We developed novel DNA extraction method "Spanning protocol" from single cell and disease-specific assay using multiplex nested PCR for Duchenne muscular dystrophy (DMD) and the combined genetic diagnosis of blastomere and polar body from the embryo. Three different DNA extraction procedures of (A), (B) and (C) were compared for efficiency ; (A) lysis in distilled water and freeze-thawing, then boiling, (B) a two-step lysis protocol involving an initial treatment in potassium hydroxide and dithiothreitol, and (C) Spanning protocol involving N-Lauroylsarcosin … More e solution. Amplification failure occurred in (A) : 47.5% , (B) : 16.25% and (C) : 5%, respectively. Spanning protocol was significantly more efficient as compared to all other methods. The efficiency of blastomere diagnosis was about 85%, whereas low false-negative rate was 5%. In final results, efficiency was increased to 99% while false-negative rate was decreased to O.1% by combined diagnosis of polar body with that of blastomere.
In another study, evaluated the clinical significance of "fetal angiography", the combined use of color and power Doppler ultrasound modalities to picture fetal vessels, from various angles. Transvaginal scanning was superior to transabdominal scanning in performing fetal angiography in pregnant patients with cephalic fetuses at the mid-trimester of gestation. Addition of fetal angiography to the conventional B-mode ultrasound was more effective in diagnosing most of fetal anomalies than the conventional B-mode alone. Another study was undertaken to determine whether introduction of B-flow, a newly-developed ultrasound modality, could lead to more understanding of the fetal placental circulation. We succeeded in three dimensional rendering of B-flow images of the fetal vasculature within the placenta for the first time. Although the quality of B-flow images of the placental vessels was satisfactory, there still remain several technical challenges to overcome for successful imaging of the fetal body vessels. However, future advances in this new technique will allow clinicians a better comprehension of the fetal vasculature. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] Miyazaki T,Yoshimura Y, et al.: "Power and colour Doppler ultrasonography for the evaluation of the vasculature of the human corpus luteum."Hum Reprod. 13. 2836-2841 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maruyama T,Yoshimura Y, et al.: "Induction of thioredoxin, a redox-active protein, by ovarian steroid hormones during growth and differentiation of endometrial stromal cells in vitro."Endocrinology. 140. 365-372 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osawa Y,Yoshimura Y, et al.: "Assessment of the dominant abnormal form is useful for predicting the outcome of intracytoplasmic sperm injection in the case of severe teratozoospermia."J Assist Reprod Genet. 16. 436-442 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiba T,Yoshimura Y, et al.: "Genetics. An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method."J Assist Reprod Genet. 16. 551-554 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hamatani T,Yoshimura Y, et al.: "A monoclonal antibody to human SP-10 inhibits in vitro the binding of human sperm to hamster oolemma but not to human zona pellucida."Biol Reprod. 62. 1201-1208 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiba T,Yoshimura Y, et al.: "Accurate multiplex polymerase chain reaction assay for gender determination from a single cell."Gynecol Obstet Invest. 49. 217-220 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉村泰典: "不妊診療プラクティス"中外医学社. 311 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉村泰典: "不妊症の病態生理と診断・治療"真興交易医書出版部. 156 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kido S, Yoshiki H, Ogawa M, Sakai Y, Yoshimura Y, Asiso S: "Clonging and characterization of mouse mSox 13 cDNA."GENE. 208. 201-206 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyakoshi K, Tanaka M, Miyazaki T, Yoshimura Y: "Prenatal ultrasound diagnosis of small-bowel torsion."Obstet Gynecol. 91. 802-803 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimura Y, Miyasoshi K, Hamatani T, Iwahashi K, Takahashi J, Kobayashi N, Sueoka K, Miyazaki, T, Kuji N, Tanaka M: "Role of β1 integrins in human endometrium and decidua during implantation."Horm Res. 50(suppl 2). 46-55 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tei C, Miyazaki T, Kuji N, Tanaka M, Sueoka K, Yoshimura Y: "Effect of danazol on the pregnancy rate in patinents with unsuccessful in vitro fertilization-embryo transfer."J Repord Med. 43. 541-546 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiokawa S, Yoshimura Y, Nagamatsu S, Sawa A, Hanashi H, Sakai K, Noguchi K, Nakamura Y: "Functional role of focal adhesion kinase in process of implantation"Mol Hum Repord. 9. 907-914 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimura Y: "Insulin-like growth factors and ovarian physiology."J Obstet Gynaeco Res. 24. 305-323 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyazaki T, Tanaka M, Miyakoshi K, Minegishi K, Kasai K, Yoshimura Y: "Power and color Doppler ultrasonography for the evaluation of the vasculature of the human corpus luteum."Hum Repord. 13. 2836-2841 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyakoshi K, Tanaka M, Gabionza D, Takamatsu K, Miyazaki T, Yuasa Y, Mukai M, Yoshimura Y: "Decidualized ovarian endometriosis mimicking malignancy."A,J.R.. 171. 1625-1626 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maruyama T, Sachi Y, Furuke K, Kitaoka Y, Kanzaki H, Yoshimura Y, Yodoi J: "Induction of thioredoxin, a redox-active protein, by ovarian steroid hormones during growth and differentiation of endometrial stromal cells in vitro."Endocrinology. 140. 365-372 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maruyama T, Yoshimura Y, Yodoi J, Sabe H: "Activation of c-Src kinase is associated with in vitro decidualization of human endometrial stromal cells."Endocrinology. 140. 2632-2636 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiokawa S, Yoshimura Y, Sawa H, Nagamatsu S, Hanashi H, Sakai K, Ando M, Nakamura Y: "Functional role of Arg-Gly-Asp (RGD)-binding sites on β1 integrin in embryo implantation using mouse blastocysts and human decidua."Biol Reprod. 60. 1468-1474 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiba T, Sueoka K, Kuroshima M, Asada H, Kuji N, Yoshimura Y: "A simplified method for lysis of single cells in PCP-based preimplantation genetic diagnosis."Jpn J Fertil Steril. 44. 253-255 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Maruyama T, Yoshimura Y, Sabe H: "Tyrosine phosphorylation and subcellular localization of focal adhesion proteins during in vitro decidualization of human endometrial stromal cells."Endocrinology. 140. 5982-5990 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osawa Y, Sueoka K, Iwata S, Shinohara M, Kobayashi N, Kuji N, Yoshimura Y: "Assessment of the dominant abnormal form is useful for predicting the outcome of intracytoplasmic sperm injection in the case of severe teratozoospermia."J Assis Reprod Genet. 16. 436-442 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakazawa K, Kanakura Y, Kometani K, Iwasaki S, Yoshimura Y: "Study on melatonin in hurman and rat placental tissue."Irophoblast Res.. 467-474 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiba T, Sueoka K, Kuroshima M, Asada H, Kuji N, Yoshimura Y: "Genetics. An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method."J Assist Reprod Genet. 16. 551-554 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimura Y: "New aspects of folliculogenesis and ovulation."Acta Obst Gynaec Jpn. 52. 584-586 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Asada H, Sueoka K, Hashiba T, Kuroshima M, Kobayashi N, Yoshimura Y: "The effects of age and abnormal sperm count on the nondisjunction of spermatozoa."J Assist Reprod Genet. 17. 51-59 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hamatani T, Tanabe K, Kamei K, Sakai N, Yamamoto Y, Yoshimura Y: "A Monoclonal antibody to human SP-10 inhibits in vitro the binding of human sperm to hamster oolemma but not to human zona pellucida."Biol Reprod. 62. 1201-1208 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ikegami N, Yoshimura Y: "Where should women deliver babies in Japan?"JAMA. 283. 2712-2714 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiba T, Sueoka K, Kuroshima M, Asada H, Kuji N, Yoshimura Y: "Accurate multiplex polymerase chain reaction assay for gender determination from a single cell."Gynecol Obstet Invest. 49. 217-220 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hanabusa H, Kuji N, Kato S, Tagami H, Kaneko S, Tanaka H, Yoshimura Y: "An evalutation of semen processing methods for eliminating HIV-1."AIDS. 14. 1611-1616 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinmoto H, Kashima K, Yuasa Y, Tanimoto A, Morikawa Y, Ishimoto H, Yoshimura Y, Hiramatsu K: "MR imaging of NON-CNS fetal abnormalities : A Pictorial essay."Radiographics. 20. 1227-1243 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Dey A, Ellenberg J, Farina A, Coleman AE, Maruyama T, Sciortino S, Lippincott-Schwartz J, Ozato K: "A bromodomain protein, MCAP, associates with mitotic chromosomes and affects G(2)-to-M transition."Mol Cell Biol. 20. 6537-6549 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimura Y: "Clinical implications of insulin-like growth factors and their binding proteins in reproduction."Endocrine J. 47. 493-515 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsubayashi H, Maruyama T, Ozawa N, Izumi S-I, Sugi T, Yoshikata K, Yoshimura Y, Makino T: "Anti-paternal antibodies by flow cytometry in the management of alloimmunization on recurrent miscarriages."Am J Reprod Immunol. 44. 284-288 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka M, Miyazaki T, Tanigaki S, Kasai K, Minegishi K, Miyakoshi K, Ishimoto H, Yoshimura Y: "Participation of reactive oxygen species in PGP_<2α>-induced apoptosis in rat Iuteal Cells."J Reprod Fert. 120. 239-245 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiokawa S, Sakai K, Akimoto Y, Suzuki N, Hanashi H, Nagamatsu S, Iwashita M, Nakamura Y, Hirano H, Yoshimura Y: "Function of the small guanosine triphosphate-binding protein Rho A in the process of implantation."J Clin Endocrinol Metab. 85. 4742-4749 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyakoshi K, Tanaka M, Gabionza D, Ishimoto H, Miyazaki T, Yoshimura Y: "Prediction fo smallness for gestational age by maternal serum human chorionic gonadotropin levels and by uterine artery doppler study."Fetal Diagn Ther. 16. 42-46 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyakoshi K, Ishimoto H, Nishimura O, Tanigaki S, Tanada M, MIyazaki T, Natori M, Yoshimura Y: "Role of leukocytes in uterine hypoperfusion and fetal growth retardation induced by ischemia-reperfusion."Am J Physiol. in press. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Asada H,Yoshimura Y, et al.: "The effects of age and abnormal sperm count on the nondisjunction of spermatozoa."J Assist Reprod Genet. 17. 51-59 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hamatani T,Yoshimura Y, et al.: "A monoclonal antibody to human SP-10 inhibits in vitro the binding of human sperm to hamster oolemma but not to human zona pellucida."Biol Reprod. 62. 1201-1208 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hashiba T,Yoshimura Y, et al.: "Accurate multiplex polymerase chain reaction assay for gender determination from a single cell."Gynecol Obstet Invest. 49. 217-220 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hanabusa H,Yoshimura Y, et al.: "An evalution of semen processing methods for eliminating HIV-1."AIDS. 14. 1611-1616 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shinmoto H,Yoshimura Y, et al.: "MR imaging of NON-CNS fetal abnormalities : A pictorial essay."Radiographics. 20. 1227-1243 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshimura Y: "Clinical implications of insulin-like growth factors and their binding proteins in reproduction"Endocr J. 47. 493-515 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉村泰典: "不妊症の病態生理と診断・治療"真興交易医書出版部. 156 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Maruyama T,Yoshimura Y et al.: "Induction of thioredoxin,a redox-active protein,by ovarian steroid hormones during growth and differentiation of endometrial stromal cells in vitro"Endocrinology. 140. 365-372 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hashiba T,Yoshimura Y et al.: "A simplified method for lysis of single cells in PCR-based preimplantation genetic diagnosis"Jpn J Fertil Steril. 44. 253-255 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kuji N,Yoshimura Y et al.: "Presence of human immunodeficiency virus in semen and its elimination by semen processing:current research"Assist Reprod. 9. 160-170 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osawa Y,Yoshimura Y et al.: "Assessment of the dominant abnormal form is useful for predicting the outcome of intracytoplasmic sperm injection in the case of severe teratozoospermia"J Assist Reprod Genet. 16. 436-442 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakazawa K,Yoshimura Y et al.: "Study on melatonin in human and rat placental tissue"Trophoblast Res. 467-474 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hashiba T,Yoshimura Y et al.: "Genetics.An Accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method"J Assist Reprod Genet. 16. 551-554 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sueoka K,Yoshimura Y et al.: "Falloposcopic tuboplasty for bilateral tubal occlusion. A novel infertility treatment as an alternative for in-vitro fertilization?" Hum Reprod. 13. 71-74 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyakoshi K,Yoshimura Y et al.: "Prenatal ultrasound diagnosis of small-bowel torsion." Obstet Gynecol. 91. 802-803 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshimura Y et al.: "Role of β_1 integrins in human endometrium and decidua during implantation." Horm Res. 50(suppl 2). 46-55 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tei C,Yoshimura Y et al.: "Effect of danazol on the pregnancy rate in patients with unsuccessful in vitro fertilization-embryo transfer." J Reprod Med. 43. 541-546 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akaboshi K,Yoshimura Y et al.: "The effects of gonadotropin-releasing hormone agonist on androstenedione production and follicular development during controlled ovarian hyperstimulation." J Assist Reprod Gene. 15. 478-484 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyazaki K,Yoshimura Y et al.: "Power and colour Doppler ultrasonography for the evaluation of the vasculature of the human corpus luteum." Hum Reprod. 13. 2836-2841 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 吉村泰典、星和彦、武谷雄二編: "不妊の基礎-病態の解明をめざして-" MEDICAL VIEW, 193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 星和彦、吉村泰典、武谷雄二編: "不妊の臨床-診断・治療の向上をめざして-" MEDICAL VIEW, 210 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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