| Project/Area Number |
10557187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KOBAYASHI Junji Tokyo Medical and Dental University,Maxillofacial Surgery, Assistant Prof., 大学院・医歯学総合研究科, 助手 (50292975)
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| Co-Investigator(Kenkyū-buntansha) |
OIDA Shinichiro Tsurumi University, Department of Biochemistry, Associate Prof., 歯学部, 助教授 (10114745)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Bone formation / Reconstructive surgery / Vascularized periosteum / rhBMP / Atelocollagen / 血管柄付き骨膜皮弁 / リコンビナントBMP / 骨再建 / 骨膜 / 人工骨膜 / BMP |
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| Research Abstract |
Various procedures have been used to treat the difficult problems of reconstruction of bone defect following the treatment of oral and maxillofacial diseases. Recently, many pedicled flaps with bone have been used for the reconstruction, but donor sites are limited. Many authors have presented the osteogenetic capacity of vascularized, or free, periosteum, but several conclusions about bone formation by the periosteum have been showed. The purposes of this study were (1) to evaluate the osteogenesis of vascularized periosteal flap combined with atelocollagen and recombinant human bone morphogenetic protein-2 (rhBMP-2) and (2) to develop the matrix filled inside that to increase the osteogenetic capacity of periosteum.
Vascularized periosteal flap of tibia of rats was elevated and atelocollagen of rhBMP-2 was rolled up by the periosteum and grafted in abdominal subcutaneous area. Histological and radiographic observations of bone formation and molecular biological examination of perioste
… More
um were made at 3 and 5 days and 1, 3, 5, 7 and 9 Weeks.
Radiologic findings indicated that a greater amount of new bone was seen in case of rhBMP-2 implantation than that of atelocollagen. But in both cases remodeling of new bone was observed between 5 and 9 weeks after surgery. Histologic observation showed that in case of atelocollagen bone was formed with membranous ossification during 2 weeks after surgery, although in case of rhBMP-2 both endochondral and membranous ossification were seen. The rate of bone formation in case of atelocollagen was faster than that of rhBMP-2. In molecular biological examination, reproducible result could not be shown, because it was difficult to resect only periosteum for sample. The experiment of implantation of rhBMP-2/PLGA capsules (rhBMP-2 with poly (DL-lactide-co-glycolide)) to subcutaneous area of rats indicated that the role of rhBMP-2 in the extraskeletal site of rats was to induce the differentiation of mesenchymal cells into the osteoblasts. Less
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