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Development of An Animal Model of the Lambert-Eaton Myasthenic Syndrome

Research Project

Project/Area Number 10557232
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 医薬分子機能学
Research InstitutionThe University of Tokyo

Principal Investigator

KIRINO Yutaka  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Professor, 大学院・薬学系研究科, 教授 (10012668)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Yoshiyuki  Eisai Co., Ltd., Tsukuba Research Laboratories, Senior Scientist, 筑波探索研究所, 主幹研究員
WATANABE Satoshi  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Assistant Professor, 大学院・薬学系研究科, 助手 (80302610)
KAWAHARA Shigenori  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Assistant Professor, 大学院・薬学系研究科, 助手 (10204752)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1999: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1998: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsNeuromuscular Junction / Autoimmune Disease / Electric Ray / Electric Organ / Synaptosome / Monoclonal Antibody / CaィイD12+ィエD1 Channel / ランバート・イートン症候群
Research Abstract

1. Development of LES animal model by immunization with electric organ synaptosomes. Mice immunized with synaptosomes isolated from the electric organ of Japanese electric ray displayed a symptom typical for the Lambert, Eaton Myasthenic Syndrome (LES) : a decrease in the quantal content of transmitter release was observed at the neuromuscular junction (NMJ) in the diaphragm from the immunized mice.
2. Development of LES animal model by passive immunization. Mice were passively immunized by injection of the serum from the actively immunized rabbits with synaptosomes. Again a decrease in the quantal content was observed, indicating that LES animal model was successfully generated.
3. Effect of the serum/IgG from IES patients to P-type Ca channels of SCLC cultured cells. The presence of P-type Ca channels of small cell lung carcinoma (SCLC) cells, which are a potential candidate for the auto-immunogen implicated in the etiology of LES, was examined by Ca imaging technique. About 20% of the cytoplasmic Ca increase induced by high KィイD1+ィエD1 stimulation was estimated to be due to P-type Ca channels as evidenced by the inhibition with a P-type specific inhibitor Agatoxin IVA. Then, the effect of LES serum to cytoplasmic Ca rise was examined but no significant inhibition was detected.
4. Effect of an acetylcholine esterase (ACE) inhibitor to the synaptic transmission at NMJ. An ACE inhibitor donepezil chloride, which is a sole effective drug for Alzheimer's disease now on market, increased the quantal content at the diaphragm NMJ. Furthermore, it was synergetic with 4-aminopyridine that is now the major medicine for LES but has a severe side effect.
5. Attempt to visualize internalization of P-type Ca channels with LES antibody. The down-regulation of P-type Ca channels with LES auto-antibody may be due to the internalization, which we attempted to visualize, if any. However, it was not successful so far.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (79 results)

All Other

All Publications (79 results)

  • [Publications] Kim, Y.I.: "An autoimmune animal model of the Lambert-Eaton syndrome"Ann. N.Y. Acad. Sci.. 841. 670-676 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoh, Y.: "Lambert-Eaton syndrome antibodies inhibit acetylcholine release and P/Q-type Ca^<2+> channels in electric ray nerve endings"J. Physiol.. 508. 427-438 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kojima, H.: "Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore"Chem. Pharm. Bull.. 46. 373-375 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iijima, K.: "cDNA isolation of Alzheimer's amyloid precursor protein from cholinergic nerve terminals of the electric organ of the electric ray"Biochem. J.. 330. 29-33 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kimura, T.: "Behavioral modulation induced by food-odor aversive conditioning and its influence on the olfactory responses of an oscillatory brain network in the slug, Limax marginatus"Learning and Memory. 4. 365-375 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kimura, T.: "Optical recording analysis of olfactory response of the PC lobe in the slug brain"Learning and Memory. 4. 389-400 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, S.: "Morphological characterization of the bursting and nonbursting neurons in the olfactory center of the terrestrial slug Limax marginatus"J. Exp. Biol.. 201. 925-930 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita, S.: "Cleavage of Alzheimer's amyloid precursor protein by secretases occurs after O-glycosylation of APP in the protein secretory pathway"J. Biol. Chem.. 273. 6277-6284 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, S.: "Differential roles of two types of voltage-gated Ca^<2+> channels in the dendrites of rat cerebellar Purkinje neurons"Brain Res.. 791. 43-55 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita, S.: "A basic amino acid in the cytoplasmic domain of Alzheimer's β-amyloid precursor protein is essential for the cleavage of APP at the α-site"J. Biol. Chem.. 273. 19304-19310 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kojima, H.: "Detection and imaging of nitric oxide with novel fluorescent indicators: Diaminofluoresceins"Anal. Chem.. 70. 2446-2453 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Lee, D-S.: "Phospholipid translocation from outer to inner leaflet of synaptic vesicle membranes isolated from the electric organ of japanese electric ray Narke japanica"J. Biochem.. 124. 798-803 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue, T.: "Selective optical recording of the neural activity in the olfactory center of land slug using a calcium indicator dye"Bioimages. 6. 59-67 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakagawa-Inoue, A.: "Odorant-evoked increase in cytosolic free calcium in cultured antenal neurons of blowflies"Zool. Sci.. 15. 661-666 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirakura, Y.: "Membrane perturbation by the Alzheimer amyloid fragment 25-35 requires aggregation"Biochem. Mol. Biol. Intnl.. 46. 787-794 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tomita, S.: "Interaction of a Neuron-specific Protein Containing PDZ Domains with Alzheimer's Amyloid Precursor Protein"J. Biol. Chem.. 274. 2243-2254 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ando, K.: "Role of Phosphorylation of Alzheimer's Amyloid Precursor Protein during Neuronal Differentiation"J. Neurosci.. 19. 4421-4427 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, S.: "Glutamate Induces Cl^- and K^+ Currents in the Olfactory Interneurons of a Terrestrial Slug"J. Comp. Physiol. A. 184. 553-562 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 桐野 豊: "学習記憶研究の戦略(シリーズ 学習・記憶の生物物理学)"放射線科学. 41. 332-335 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 川原茂敬: "神経細胞におけるカルシウムイメージング"細胞. 30. 7-10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺 恵: "ナメクジの嗅覚-味覚連合学習(シリーズ 学習・記憶の生物物理学)"放射線科学. 42. 26-34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 川原茂敬: "瞬目反射条件付けと小脳・海馬(シリーズ 学習・記憶の生物物理学)"放射線科学. 42. 252-259 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 川原茂敬: "遺伝子変異マウスの瞬目反射条件付け(特集号「脳・神経研究の最先端-その発生機構から高次脳機能まで」)"実験医学増刊. 17(16). 153-158 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺 恵: "ナメクジにおける嗅覚-味覚連合学習(特集号「神経回路形成と機能発達」)"蛋白質核酸酵素. 45. 380-386 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 桐野 豊 編著: "基礎薬学・物理化学 スタディガイド"廣川書店. 219 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 桐野 豊 編著: "基礎薬学・物理化学(改訂版)"廣川書店. 460 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 桐野 豊 編著: "生命薬学シリーズ・物理化学(上)"共立出版. 217 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 桐野 豊 編著: "生命薬学シリーズ・物理化学(下)"共立出版. 250 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺 恵、桐野 豊: "無脊椎動物の脳の働き「脳研究への招待」(小幡邦彦 編)"共立出版. ((印刷中))

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ko-ichi Iijima, Dong-Seok Lee, Jun-ichi Okutsu, Susumu Tomita, Naohide Hirashima, Yutaka Kirino and Toshiharu Suzuki: "Phosphorylated Form of Alzheimer_s Amyloid Precursor protein Exists in Cholinergic Nerve Termini from Electric Ray Electric Organ"Biochem. J.. 330. 29-33 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Susumu Tomita, Yutaka Kirino and Toshiharu Suzuki: "Cleavage of Alzheimer_s Amyloid Precursor Protein by Secretases Occurs after O-Glycosylation of APP in Protein Secretory Pathway"J. Biol. Chem.. 273. 6277-6284 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirotatsu Kojima, Kuniko Sakurai, Kazuya Kokuchi, Shigenori Kawahara, Yutaka Kirino, Hiroshi Nagoshi, Yasunobu Hirata, and Tetsuo Nagano: "Development of a Fluorescent Indicator for Nitric Oxide Based on the Fluorescein Chromophore"Chem. Pharm. Bull.. 46. 373-375 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasushi Satoh, Naohide Hirashima, Hiroshi Tokumaru, Masanori P. Takahashi, Jin Kang, Michael P. Viglione, Yong I. Kim, and Yutaka Kirino: "Lambert-Eaton Syndrome Antibodies Inhibit Acetylcholine Release and P/Q-type CaィイD2+ィエD2 Channels in Electric Ray Nerve Endings"J. Physiol.. 508. 427-438 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Kimura, Shoichi Toda, Tatsuhiko Sekiguchi and Yutaka Kirino: "Behavioral Modulation Induced by Food-Odor Aversive Conditioning, and Its Influence on the Olfactory Responses of an Oscillatory Brain Network in the Slug, Limax marginatus"Learning and Memory. 4. 365-375 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tetsuya Kimura, Shoichi Toda, Tatsuhiko Sekiguchi, Shigenori Kawahara and Yutaka Kirino: "Optical Recording Analysis of Olfactory Response of the PC Lobe in the Slug Brain"Learning and Memory. 4. 389-400 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoshi Watanabe, Shigenori Kawahara and Yutaka Kirino: "Morphological Characterization of the Bursting and Nonbursting Neurones in the Olfactory Center of the Terrestrial Slug Limax marginatus"J. Exp. Biol.. 201. 925-930 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yong I. Kim, Eric H. Middlekauff, Michael P. Viglione, Jun-ichi Okutsu, Yasushi Satoh, Naohide Hirashima and Yutaka Kirino: "An Autoimmune Animal Model of the Lambert-Easton Syndrome"Ann. N. Y. Acad. Sci.. 841. 670-676 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shigeo Watanabe, Hiroshi Takagi, Tsugumichi Miyasho, Masashi Inoue, Yutaka Kirino, Yoshihisa Kudo and Hiroyoshi Miyakawa: "Differential Roles of Two Types of Voltage-gated CaィイD12+ィエD1 Channels in the Dendrites of Rat Cerebellar Purkinje Neurons"Brain Res.. 791. 43-55 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Susumu Tomita, Yutaka Kirino and Toshiharu Suzuki: "A Basic Amino Acid in the Cytoplasmic Domain of Alzheimer's β-Amyloid Precursor Protein (APP) Is Essential for Cleavage of APP at the α-Site"J. Biol. Chem.. 273(30). 19304-19310 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirotatsu Kojima, Naoki Nakatsubo, Kazuya Kikuchi, Shigenori Kawahara, Yutaka Kirino, Hiroshi Nagoshi, Yasunobu Hirata and Tetsuo Nagano: "Detection and Imaging of Nitric Oxide with Novel Fluorescent Indicators : Diaminofluoresceins"Anal. Chem.. 70. 2446-2453 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Dong-Seok Lee, Kazunori Anzai, Naohide Hirashima, Ken-ichi Hatanaka, Miho Nakamura, Yasuyuki Yoshioka and Yutaka Kirino: "Phospholipid Translocation from Outer to Inner Leaflet of Synaptic Vesicle Membranes Isolated from the Electric Organ of Japanese Electric Ray"Narke japonica, J. Biochem.. 124(4). 798-803 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuyoshi Inoue, Shigenori Kawahara, Shoichi Toda, Satoshi Watanabe and Yutaka Kirino: "Selective Optical Recording of the Neural Activity in the Olfactory Center of Land Slug Using a Calcium Indicator Dye"Bioimages. 6(2). 59-67 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Azusa Nakagawa-Inoue, Shigenori Kawahara, Yutaka Kirino, and Tatsuhiko Sekiguchi: "Odorant-evoked increase in cytosolic free calcium in cultured antennal neurons of blowflies"Zool. Sci.. 15. 661-666 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yutaka Hirakura, Yasushi Satoh, Toshiharu Suzuki and Yutaka Kirino: "Membrane Perturbation by the Alzheimer Amyloid Fragment β25-35 Requires Aggregation"Biochem. Mol. Biol. Intnl.. 46(4). 787-794 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Susumu Tomita, Toshinori Ozaki, Hidenori Taru, Shinobu Oguchi Shizu Takeda, Yoshimasa Yagi, Shigeru Sakiyama, Yutaka Kirino, and Toshiharu Suzuki: "Interaction of a Neuron-specific Protein Containing PDZ Domains with Alzheimer's Amyloid Precursor Protein"J. Biol. Chem.. 274. 2243-2254 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Satoshi Watanabe, Shigenori Kawahara and Yutaka Kirino: "Glutamate Induces ClィイD1-ィエD1 and K ィイD1+ィエD1 Currents in the Olfactory Interneurons of a Terrestrial Slug"J. Comp. Physiol. A. 184. 553-562 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanae Ando, Yutaka Kirino and Toshiharu Suzuki: "Role of Phosphorylation of Alzheimer's Amyloid Precursor Protein during Neuronal Differentiation"J. Neurosci.. 19. 4421-4427 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Susumu Tomita: "Interaction of a Neuron-specific Protein Containing PDZ Domains with Alzheimer's Amyloid Precursor Protein"J.Biol.Chem.. 274. 2243-2254 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kanae Ando: "Role of Phosphorylation of Alzheimer's Amyloid Precursor Protein during Neuronal Differentiation"J.Neurosci.. 19. 4421-4427 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Satoshi Watanabe: "Glutamate Induces C1^- and K^+ Currents in the Olfactory Interneurons of a Terrestrial Slug"J.Comp.Physiol.A. 184. 553-562 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 渡辺 恵: "ナメクジの嗅覚-味覚連合学習(シリーズ 学習・記憶の生物物理学)"放射線科学. 42. 26-34 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 川原茂敬: "瞬目反射条件付けと小脳・海馬(シリーズ 学習・記憶の生物物理学)"放射線科学. 42. 252-259 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 川原茂敬: "遺伝子変異マウスの瞬目反射条件付け(特集号「脳・神経研究の最先端-その発生機構から高次脳機能まで」)"実験医学増刊. 17(16). 153-158 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 渡辺 恵: "ナメクジにおける嗅覚-味覚連合学習(特集号「神経回路形成と機能発達」)"蛋白質核酸酵素. 45. 380-386 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 桐野 豊 編著: "基礎薬学・物理化学 スタディガイド"廣川書店. 219 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 桐野 豊 編著: "基礎薬学・物理化学(改訂版)"廣川書店. 460 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 桐野 豊 編著: "生命薬学シリーズ・物理化学(上)"共立出版. 217 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 桐野 豊 編著: "生命薬学シリーズ・物理化学(下)"共立出版. 250 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 渡辺 恵、桐野 豊: "無脊椎動物の脳の働き「脳研究への招待」(小幡邦彦 編)"共立出版(印刷中).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ko-ichi Iijima: "Phosphorylated Form of Alzheimer's Amyloid Precursor Protein Exists in Cholinergic Nerve T ermini from Electric Ray Electric Organ" Biochem.J.330. 29-33 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Susumu Tomita: "Cleavege of Alzheimer's Amyloid Precursor Protein by Secretases Occurs after O-glycosyla-tion of APP in Protein Secretory Pathway" J.Biol.Chem.273. 6277-6284 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirotasu Kojima: "Development of a Fluorescent Indicator for Nitric Oxide Based on the Fluorescein Chromophore" Chem.Pharm.bull.46. 373-375 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasushi Satoh: "Lambert-Eaton Syndrome Antibodies Inhibit Acetylcholine Release and P/Q-type Ca^<2+> Channels in Electric Ray Nerve Endings" J.Physiol.508. 427-438 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tetsuya Kimura: "Behavioral Modulation Induced by Food-Odor Aversive Conditioning,and Its Influence on the Olfactory Responses of an Oscillatory Brain Network in the Slug, Limax marginatus" Learning and Memory. 4. 365-375 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tetsuya Kimura: "Optical Recording Analysis of Olfactory Response of the PC Lobe in the Slug Brain" Learning and Memory. 4. 389-400 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Satoshi Watanabe: "Morphological Characterization of the Bursting and Nonbursting Neurons in the Olfactory Cent er of the Terrestrial Slug Limax marginatus" J.Exp.Biol.201. 925-930 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yong I.Kim: "An Autoimmune Animal Model of the Lambert-Eaton Syndrom" Ann.N.Y.Acad.Sci. 841. 670-676 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shigeo Watanabe: "Differential Roles of Two Types of Voltage-gated Ca^<2+> Channels in the Dendrites of Rat Cerebellar Purkinje Neurons" Brain Res.791. 43-55 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Susumu Tomita: "A Basic Amino Acid in the Cytopolasmic Domain of Altzheimer's β-Amyloid Precursor Protein(APP)Is Essential for Cleavage of APP at the α-site" J.Biol.Chem.273. 19304-19310 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirotatsu Kojima: "Detection and Imaging of Nitric Oxide with Novel Fluorescent Indicators: Diaminofluoresceins" Anal.Chem.70. 2446-2453 (1998)

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      1998 Annual Research Report
  • [Publications] 桐野 豊: "学習記憶研究の戦略" 放射線医学. 38. 174-177 (1998)

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      1998 Annual Research Report
  • [Publications] Dong-Seok Lee: "Phospholipid Translocation from Outer to Inner Leaflet of Synaptic Vesicle membranes Isolated from the Electric Organ of Japanese Electric Ray Narke japonica" J.Biochem.124. 798-803 (1998)

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      1998 Annual Research Report
  • [Publications] Tsuyoshi Inoue: "Selective Optical Recording of the Neural Activity in the Olfactory Center of Land Slug Using a Calcium Indicator Dye" Bioimages. 6. 59-67 (1998)

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      1998 Annual Research Report
  • [Publications] Azusa Nakagawa-Inoue: "Odorant-evoked Increase in Cytosolic Free Calcium in Cultured Anntenal Neurons of Blowflies" Zool.Sci.15. 661-666 (1998)

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      1998 Annual Research Report
  • [Publications] Yutaka Hirakura: "Membrane Perturbation by the Alzheimer Amyloid Fragment β25-35 Requires Aggregation" Biochem.Mol.Biol.Intnl.46. 787-794 (1998)

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      1998 Annual Research Report
  • [Publications] Susumu Tomita: "Interaction of a Neuron-specific Protein Containing PDZ Domains with AlzHeimer's Amyloid Precursor Protein" J.Biol.Chem.274. 2243-2254 (1999)

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      1998 Annual Research Report
  • [Publications] Satoshi Watanabe: "Glutamate Induces Cl^- and K^+ Currents in the Olfacotry Interneurons of a Terrestrial Slug" J.Comp.Physiol.A. (in press). (1999)

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      1998 Annual Research Report
  • [Publications] Kanae Ando: "Role of Phsphorylation of Alzheimer's Amyloid Precursor Protein during Neuronal Differentiation" J.Neurosci.(in press). (1999)

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      1998 Annual Research Report
  • [Publications] 桐野 豊: "基礎薬学 物理化学(改訂版)" 廣川書店(in press), (1999)

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      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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