| Project/Area Number |
10557234
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
医薬分子機能学
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| Research Institution | Osaka University |
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Principal Investigator |
YUTSUDO Masuo (2001) Research Institute for Microbial Diseases, Osaka University, Associate Professor, 微生物病研究所, 助教授 (70135747)
中西 真人 (1998-2000) 大阪大学, 微生物病研究所, 助教授 (10172355)
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| Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Mamoru DNAVEC Research Inc., Director, 所長(研究職)
NAKANISHI Mahito National Institute for Advanced Industrial Sciences and Technology, Principal Investigator, ジーンディスカバリー研究センター, 主任研究員 (10172355)
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| Project Period (FY) |
1998 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | DNA / Nuclear targeting / Phage / Telomere / Cancer / Sendai virus / Fusogenic liposome / Gene therapy / TRF1 / 染色体 / ハイブリッドベクター / ガン胎児抗原 / ナトリウムチャンネル / ラムダファージ / 細胞膜 / 核膜 / Protein Transduction Domain / 人工染色体 / RNAレプリコン / 遺伝子導入 / ベクター |
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| Research Abstract |
In this research project, we investigate the essential function of biological systems, such as viruses and chromosomes, which will be useful for developing novel non-viral devices for gene transfer and expression. The results we have obtained during the term of this project are summarized as below.
1. We analyzed the mechanism of membrane fusion that will mediated efficient DNA delivery, and found that the membrane fusion requires essential non-receptor molecules which may be interacted with HN protein of Sendai virus.
2. We establish the system to display various biological peptides on the surface of lambda phage particles, and propose to use this system to analyze the function of these peptides as components of non-viral gene delivery vehicles. Using this system, we showed that Tat peptide could enhance the delivery of lambda phage DNA into the cells and that NLS signal peptide of SV40 T antigen could target the lambda phage particles into the nucleus through the nuclear pore complex.
3. We analyze the mechanism by which human chromosomes are stabilized in the nucleus, and found that the interaction between TRF1 and telomere repeat was involved in this process.
4. We succeeded to cure mice with the disseminated cancer by treating them with the fusogenic liposomes encapsulating cancer-specific suicide gene.
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