| Project/Area Number |
10558096
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
HIRAMA Masahiro Tohoku University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (30165203)
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| Co-Investigator(Kenkyū-buntansha) |
OGURI Hiroki Tohoku University, Graduate School of Science, Assistant Professor, 大学院・理学研究科, 助手 (80311546)
TOMIOKA Yoshihisa Tohoku University, Tohoku University Hospital, Assistant Professor, 医学部・附属病院, 助手 (00282062)
MIZUGAKI Michinao Tohoku University, Tohoku University Hospital, Professor, 医学部・附属病院, 教授 (60004595)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1998: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | ciguatoxin / antibody / hapten / antigen / gene / synthesis / olefin metathesis / ELISA / モノクローナル抗体 / 人工抗原 / シガテラ / 抗シガトキシン抗体 / 海産ポリエーテル / コンジュゲート / 免疫 |
|---|
| Research Abstract |
The ABC-ring fragment of ciguatoxin was synthesized as a C2 epimeric mixture (1 : 1 ). The mixture, which was designed as a possible hapten for preparing an anti-ciguatoxin monoclonal antibody, was conjugated with a carrier protein (KLH) and then used for immunization of mice. The three monoclonal antibodies (mAbs) obtained have shown an appreciable reactivity to the hapten. Each mAb discriminated configuration of the C2 hydroxy group of the ABC-ring fragment. The mAb which showed reactivity to the 2S-fragment was cross-reacted with ciguatoxin. Furthermore, new and efficient synthetic methods utilizing olefin methathesis were developed and several designed haptens have been prepared and conjugated with KLH and BSA, which have been used for immunization of mice.
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