| Project/Area Number |
10558108
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biophysics
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| Research Institution | Department of Applied Physics, Graduate School of Engineering, NAGOYA University |
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Principal Investigator |
ISHIJIMA Akihiko Department of Applied Physics, Graduate School of Engineering, NAGOYA University, Associate Professor, 工学研究科, 助教授 (80301216)
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| Co-Investigator(Kenkyū-buntansha) |
日比野 政宏 (日比野 政裕) 名古屋大学, 大学院・工学研究科, 助手 (00242834)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Optical tweezers / Nano-manipulation / Single molecule measurement / Chara myosin / ATP / 人工平面膜 / 脂質 / 拡散定数 / 鞭毛モーター / 光ピンセット / 1分子イメージング / 1分子ナノマニピュレーション / エバネンセント場 / 生体分子 / アクチン / ミオシン / エネルギー変換 |
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| Research Abstract |
In Characean cells, the endoplasm streams along the actin cables are at 30-100 μm/s. This sliding velocity is about 5 times faster than no load shortening velocity of skeletal muscle(10 μm/s). However, the mechanism about this high sliding speed of the endoplasm streams haven' t been understood yet. We want to perform dynamic measurement on single molecular level of this Chara myosin to elucidate the molecular mechanism that can produce high sliding speed.
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