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Establishment of functional germ cell precursor cell lines

Research Project

Project/Area Number 10558118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory animal science
Research InstitutionResearch Institute, Osaka Medical Center for Maternal and Child Health

Principal Investigator

MATSUI Yasuhisa  Research Institute, Osaka Medical Center for Maternal and Child Health, Department of Embryology, Director of the department, 病因病態部門, 部長 (40241575)

Co-Investigator(Kenkyū-buntansha) 矢野 環  大阪府立母子保健総合医療センター研究所, 病因病態部門, 流動研究員
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsprimordial germ cell / spermalogonia / EG cell / bcl-2 / EGF / Bcl-2 / トランスジェニックマウス / 配偶子 / 細胞株 / p53
Research Abstract

The aim of this research is to derive cell lines from mouse primordial germ cells (PGCs) and spermatogonia and to differentiate them into functional gametes. For this purpose, Ifirst examined whether PGC-derived pluripotential EG cells could redifferentiate into PGCs when culturing them with somatic cells from embryonic gonads. After culture, I stained them with PGC-specific vasa antibody, and found that EG cells differentiated to vasa-positive cells. In addition, EGF could also induced vasa-positive cells from EG cells without gonadal somatic cells. I also attempted to establish spermatogonial cell lines form a transgenic mice overexpressing bcl-2 in spermatogonia. Dissociated transgenic testicular cells were cultured on a feeder layer of STO cells and I found that TRA98-positive germ cells determined by imnmunostaining could be detected up to 45 days after culture, while wild type testicular cells survived up to only 30 days in culture. In addition, I found that the transgenic germ cells differentiated intoTRA369-positive spermatocytes and/or spermatid after 10 days in culture.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Yoshimizu, T. et al.: "Germline-specific expression of the Oct-4 /green fluorescent protein (GFP) transgene in mice"Development Growth and Differentiation. 41. 675-684 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toyoda-Ohno, H. et al.: "Members of ErbB receptor tyrosine kinases are in involved in germ cell development in fetal mouse gonads."Developmental Biology. 215. 399-406 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohta, T. et al.: "Hematopietic development of the primordial germ cell-derived mouse embryonic germ cells in culture."Biochem.Biophys.Res.Commn.. 260. 475-482 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toyooka, Y. et al.: "Expression and intracellular localization of mouse Vasa-homolog protein during germ cell development."Mechanisms of Development. 93. 139-149 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsui, Y. et al.: "Apoptosis of fetal testicular cells is regulated by both p53-dependent and independent mechanisms."Molecular Reproduction and Development. 55. 399-405 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sygiyama, N. et al.: "Bcl-2 inhibits apoptosis of spermatogonia and growth of spermatogonial stem cells in cell-intrinsic manner"Molecular Reproduction and Development. 58. 30-38 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 松居靖久: "動物発生工学"朝倉書店. 270 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimizu, T. et al.: "Germline-specific expression of the Oct-4 /green fluorescent protein (GFP) transgene in mice."Development growth and differentiation,. 41. 675-684 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toyoda-Ohno, H. et al.: "Members of ErbB receptor tyrosine kinases are in involved in germ cell development in fetal mouse gonads."Developmental Biology. 215. 399-406 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohtaka, T., et al.: "Hematopietic development of the primordial germ cell-derived mouse embryonic germ cells in culture."Biochem.Biophys.Res.Commn.. 260. 475-482 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toyooka, Y, et al.: "Expression and intracellular localization of mouse Vasa-homolog protein during genii cell development."Mechanisms of Development. 93. 139-149 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsui, Y., et al.: "Apoptosis of fetal testicular cells is regulated by both p53-dependent and independent mechanisms."Molecular Reproduction and Development. 55. 399-405 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugiyamna, N., et al.: "Bcl-2 inhibits apoptosis of spermnatogonia and growth of spermnatogonial stein cells in cell-intrinsic manner"Molecular Reproduction amid Development. 58. 30-38 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimizu,Y.: "Germline-specific expression of the Oct-4/green fluorescent protein (GFP) transgene in mice."Development Growth and Differentiation. 41. 675-684 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohno,H.: "Members of ErbB receptor tyrosine kinases are involved in germ cell development in fetal mouse gonads."Developmental Biology. 215. 399-406 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsui,Y: "Apoptosis of fetal testicular cells is regulated by both p53-dependent and independent mechanisms."Molecular Reproduction and Development. 55. 399-405 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toyooka,Y.: "Expression and intracellular localization of mouse Vasa-homolog protein during germ cell development."Mechanism of Development. 93. 139-149 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugiyama,N.: "Bcl-2 inhibits apoptosis of spermatogonia and growth of spermatogonoal stem cells in cell-intrinsic manner."Molecular Reproduction and Development. 58. 30-38 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshimizu,T.: "Stage-specific tissue and cell interaction play key roles for mouse germ cell specification."Development. 128. 481-490 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohtaka, T. et al.: "Hematopietic development of the primordial germ cell-derived mouse embryonic germ cells in culture"Biochem. Biophys. Res. Commn.. 260. 475-482 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimizu, T. et al.: "Germline-specific expression of the Oct-4/green fluorescent (GFP) transgene in mouse"Dev. Growth Differ. 41. 675-684 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toyoda-Ohno, H. et al.: "Members of ErbB receptor fyrosine kinases are involved in germ cell development in fetal mouse gonads"Dev. Biol.. 215. 399-406 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsui, Y. et al.: "Apoptosis of fetal testicular cells is regulated by both p53-dependent and independent mechanisms"Mol. Reprod. Dev. 55 (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toyooka, Y. et al.: "Expression and intracellular localization of mouse V asa-homolog protein during germ cell development"Mech. Dev.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsui, Y.: "Regulation of germ cell death in mammalian gonads." APMIS. 106. 142-148 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matusi, Y.: "Developmental fate of the mouse germ cells." Int.J.Dev.Biol.42. 1037-1042 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hidai, C.: "Cloning and characterization of developmental endothelial locus-1: An embryonic endothelial cell protein that binds the αvβ3 integrin receptor." Genes Dev.12. 21-33 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大野裕子: "始原生殖細胞の増殖制御機構" 大阪府立母子保健総合医療センター雑誌. (印刷中).

    • Related Report
      1998 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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