• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of practically usefull Ws/Ws rat and its utilization

Research Project

Project/Area Number 10558121
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory animal science
Research InstitutionOsaka University

Principal Investigator

HIROTA Seiichi (2000)  Osaka Univ.Grad.Sch.Med.Associate professor, 医学系研究科, 助教授 (50218856)

北村 幸彦 (1998-1999)  大阪大学, 医学系研究科, 教授 (70028520)

Co-Investigator(Kenkyū-buntansha) ASAI Hidekazu  Japan SLC Co.Ltd.Head of Haruno Branch, 春野支店長(研究職)
MORII Eiichi  Osaka Univ.Grad.Sch.Med.Assistant professor, 医学系研究科, 助手 (10283772)
JIPPO Tomoko  Osaka Univ.Grad.Sch.Med.Assistant professor, 医学系研究科, 助手 (70252658)
廣田 誠一  大阪大学, 医学系研究科, 助教授 (50218856)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥8,100,000 (Direct Cost: ¥8,100,000)
Keywordsc-kit gene / mutant rats / Ws / intrapyloric pressure / fibrosis / memory / Wsラット / 中枢神経系 / 海馬 / c-kit レセプター / マスト細胞欠損ラット / マスト細胞 / 突然変異ラット / 消化管ストローマ細胞腫 / レセプターチロシンキナーゼ / カハール介在細胞 / c-kit / 近交系ラット / 受容体チロシンキナーゼ
Research Abstract

Ws/Ws rat is only the mutant of rat c-kit gene that we first found in the world in 1991. The c-kit gene encodes a receptor tyrosine kinase, KIT.And the ligand for KIT is stem cell factor, SCF.The KIT-SCF system is essential for development of mast cells and interstitial cell of Cajal(ICC). At present, brothers and sisters of rats that have Ws mutation are crossed each other through several generations in SLC Japan Co.Ltd.Ws/Ws rats will be stably available in near future.
We carried out several investigations using Ws/Ws rats. First, the function of pyloric ring was examined by measuring intrapyloric pressure. In ICC-deficient Ws/Ws rats, the contraction pressure did not prolong as observed in +/+ rats, and spike-like waves were noted. The abnormal spike-like waves were considered to be a cause of bile reflux into stomach observed in Ws/Ws rats. Second, the role of mast cells in fibrosis was examined using mast cell-deficient Ws/Ws rats. In both lung and liver fibrosis models, fibrosis in Ws/Ws rats was more prominent than that in +/+ rats, suggesting that the increase of mast cells in fibrotic tissues is not a cause but a result of fibrosis. Third, we examined the role of KIT receptor in central nervous system by using Ws/Ws rats. Morris maze task revealed the impairment of spatial learning and the electrophysiological examination of hippocampal slice showed the impairment of synaptic potentiation in Ws/Ws rats.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Nakama A: "Disturbed pyloric motility in Ws/Ws mutant rats due to deficiency of c-kit-expressing interstitial cells of cajal"Pathol Int. 48. 843-849 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirota S: "Gain-of-function mutations of c-kit in human gastrointestinal stromal tumors."Science. 279. 577-580 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okazaki T: "Increase of mast cells in the liver and lung may be associated with but not a cause of fibrosis : demonstration using mast cell-deficient Ws/Ws rats"Lab Invest. 78. 1431-1438 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishida T: "Familial gastrointestinal stromal tumors with gesmline mutation of KIT"Nature Genet. 19. 323-324 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katafuchi T: "Impairment of spatial learning and hippocampal synaptic potentiation in c-kit mutant rats"Learn Mem. 7. 383-392 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirota S: "Gain-of-function mutation at extra cellular domain of KIT in gastrointestinal stromal tumors"J Pathol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakama A, et al: "Disturbed pyloric motility in Ws/Ws mutant rats due to deficiency of c-kit-expressing intersititial cells of Cajal."Pathol Int.. 48. 843-849 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okazaki T, et al: "Increase of mast cells in the liver and lung may be associated with but not a cause of fibrosis : demonstration using mast cell-deficient Ws/Ws rats."Lab Invest.. 78. 1431-1438 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirota S, et al: "Gain-of-function mutations of c-kit in human gastrointestinal stromal tumors."Science.. 279. 577-580 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishida T, et al: "Familial gastrointestinal stromal tumors with germ line mutation of KIT."Nature Genet.. 19. 323-324 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Taniguchi M, et al: "Effect of c-kit mutation on prognosis of gastrointestinal stromal tumors."Cancer Res.. 59. 4297-4300 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirota S, et al: "Cause of familial and multiple gastrointestinal autonomic nerve tumors with hyperplasia of interstitial cells of Cajal is germline mutation of the c-kit gene."Am J Surg Pathol.. 24. 326-327 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katafuchi T, et al: "Impairment of spatial learning and hippocampal synaptic potentiation in c-kit mutant rats."Learn Mem.. 7. 383-392 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Isozaki K, et al: "Germ-line activating mutation in the kinase domain of c-kit gene in familial gastrointestinal stromal tumors."Am J Pathol.. 157. 1581-1585 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirota S, et al: "Gain-of-function mutation at extracellular domain of KIT in gastrointestinal stromal tumors."J Pathol.. (In press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katafuchi T.: "Impairment of spatial learning and nippocampal synaptic potentiation in c-kit mutant rats."Learn Mem.. 7. 383-392 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Isozaki K.: "germ-line activating mutation in the kinase domain of c-kit gene in familial gastrointestinal stromal tumors."Am J Pathol. 157. 1581-1585 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirota S: "Gain-of-function mutation at extracellular domain of KIT in gastrointestinal stromal tumors."J Pathol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taniguchi M, Kitamura Y et al.: "Effect of c-kit mutation on prognosis of gastrointestinal stromal tumors"Cancer Research. 59. 4297-4300 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ito A,Kitamura Y et al.: "Inhibitory effect of transcription factor encoded by the mi allele in mast cells"Blood. 93. 1189-1196 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsujimura Y,Kitamura Y et al.: "Activating mutation in the catalytic domain of c-kit elicts hematopoietic transformation"Blood. 93. 1319-1329 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kim DK,Kitamura Y et al.: "Different effect of various mutant MITF on phenotype of murine mast cells"Blood. 93. 4179-4186 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Adachi S,Kitamura Y et al.: "Involvement of MITF in expression of alpha melanocyte stimulating hormone receptor"Journal of Immunology. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirota S,Kitamura Y et al.: "Cause of familiar and multiple gastrointestinal autonomic nerve tumors"American Journal of Surgical Pathology. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitamura Y et al.: "Signal Transduction in Mast Cells and Basophils"Springer-Verlag,New York. 423 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitamura Y et al.: "Mast Cells and Basophils in Physiology ,Pathology,and Host Defense"Academic Press,London(in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirota S,Kitamura Y,et al: "Gain-of-function mutations of c-kit in human gastro-intestinal stromal tumors" Sience. 279. 577-580 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishida T,Kitamura Y et al: "Familial gastrointestinal stromal tumors with germ line mutation of KIT" Nature Genetics. 19. 323-324 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okazaki T,Kitamura Y,et al: "Increase of mast cells in liver and lung may be associated with but not a cause of fibrosis" Laboratory Investigation. 78. 1431-1438 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakama H,Kitamura Y,et al: "Disturbed pyloric motility in Ws/Ws rats due to deficiency of c-kit-expressing Cajal cells" Pathology International. 48. 843-849 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Berin MC,Kitamura Y,et al: "The influence of mast cells on pathways of transepithelial antigen transport in rat intestine" Journal of Immunology. 161. 2561-2566 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakahara M,Kitamura Y,et al: "A novel gain-of-function mutation of c-kit in gastro-intestinal stromal tumors" Gastroenterology. 115. 1090-1095 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitamura Y,et al: "Hematopoietic Lineages:Regulation of Cell Production and Development" marcel dekker,New York(in press),

    • Related Report
      1998 Annual Research Report
  • [Publications] Kitamura Y,et al: "Signal Transduction in Mast Cells and Basophils" Springer-Verlag,New York(in press),

    • Related Report
      1998 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi