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Visualization and quantification of intracellular magnesium ion to determine the influence of magnesium to pragnancy circulation

Research Project

Project/Area Number 10558134
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

ARAKI Tsutomu  Engineering Science, Osaka Univ., Prof., 基礎工学研究科, 教授 (50136214)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Mamoru  Engineering Science, Osaka Univ., Lect., 基礎工学研究科, 講師 (70237949)
YAMAZAKI Mineo  Nara Medical University, Assoc. Prof., 医学部, 助教授 (00220301)
TOHNO Yoshiyuki  Nara Medical University, Prof., 医学部, 教授 (40075023)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥7,500,000 (Direct Cost: ¥7,500,000)
KeywordsMAGUNESIUM ION / CALCIUM ION / FLUORESCENCE / MICRO-FLUOROMETER / PREECLAMPTIC PRAGNANT WOMAN / VISUALIZATION / RAMAN SPECTROSCOPY / 時間分解測光 / 組織蛍光 / 自己蛍光 / マイクロ波誘起プラズマ発光分析
Research Abstract

One aim of this project is to develop a microphotometric measurement system that can visualize and quantify the intracellular magnesium ion level, and to apply this system into the study on the dynamics of magnesium ion during the pregnancy circulation. To visualize the intracellular magnesium ion, a fluorescent probe (Mag-fra2) is used in conjunction with a micro spectrophotometer that has two excitation wavelengths to take an intensity ratio of the fluorescence emission. Because the calcium ion level and the magnesium ion level is competetive in the cell, both calcium level and magnesium level must be measured with the same apparatus. The test apparatus has been completed. Relationship between the magnesium ion level in thrombocyte and the pregnancy has been investigated in preeclamptic pragnant women.
The project has been extended to quantify the calcium in human blood vessels. The calcium is quantified by atomic emission spectroscoy (AES). For this, a microwave-induced coupled AES system has been made. The calcium content of lower limbs was higher than that of upper limbs. This result indicates that correlation between Ca content and arteriosclerosis maybe exists. Ca was much contained in parts of veinlet and joint in the artery comparing with other portion. This indicates that correlation between Ca content and mechanical stress such as exercise (living custom), blood pressure, and blood turbulent flow maybe exists.
Also a new technique that can visualize the conformation change of molecules in living sample has been developed. This technique is based on the microscopic optics and coherent anti-Stokes Raman spectroscopy (CARS). To establish the microscopic CARS method, optical transfer function of the CARS system has been studied.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Y. Tateyama, et al.: "Correlation calcium accumulations in arteries, veins, cartilages, ligaments, and bones in single humans"Biological Three Element Research. 74(印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 桝田充彦、山崎峰夫他: "妊娠中毒症妊婦における細胞内Mgイオン濃度の動態"妊娠中毒症学会誌. 印刷中. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Matsumoto, S. Kitamura and T. Araki: "Autofluorescence in human dentin in relation to age, tooth type and temperature measured by nanosecond time-resolved fluorescence microscopy"Archieves of Oral Biology. 44. 309-318 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Hashimoto and T. Araki: "Coherent antistokes Raman scattering microscopy"SPIE. 3749. 496-497 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Chikamori, T. Araki, and K. Sato: "Phosphohydrolytic activity in Paramecium Caudatum at neutral pH"Acta Histochemica.. Vol.98. 395-408 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Hashimoto and T. Araki: "Coherent antistokes Raman scattering microscopy"SPIE. Vol.3749. 496-497 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Matsumoto, S. Kitamura, and T. Araki: "Autofluorescence in human dentine measured by nanosecond time-resolved fluorescence microscopy : relation to age, tooth species and temerature"Archives of Oral Biology. Vol.44. 309-318 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Tateyama, Y. Takano, Y. Tohno, Y. Moriwake, S. Tohno, and T. Araki: "Correlation of calcium accumulations in arteries,veins, cartilages, ligaments, and bones in single humans"Biol. Trace Element Res. Vol.74 (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Tateyama,et al.: "Correlation of calcium accumulations in arteries,veins,cartilages,ligaments,bones in single humans"Biological Trace Element Research. 74(印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田 彦、山崎峰夫 他: "妊娠中毒症妊婦における細胞内Mgイオン濃度の動態"妊娠中毒症学会誌. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H. Matsumoto,S. Kitamura, and T. Arai: "Autofluorescence in human dentin in relation to age,tooth type and temperature measured by nanosecond time-resolved fluorescence microscopy"Archieves of Oral Biology. 44. 309-318 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Hashimato and T. Arai: "Coherent antistokes Raman scattering microscopy"SPIE. 3749. 496-497 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Chikamori,T.Araki and K.Sato: "Phosphohydrolytic activity in paramecium candatum at neutral pH" Acta Histochemica. 100巻. 395-408 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Matsumoto,S.Kitamura and T.Araki: "Autofluorescence in human dentine measured by nanosecond time-resolved fluorescence microscopy" Archs.Oral Biol.44巻(印刷中). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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