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Improvement of creep meter to estimate physical properties of cell wall for selection of crop cultivars

Research Project

Project/Area Number 10559012
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 広領域
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

YAMAGUCHI Junji  Nagoya University, BioScience Center, Associate Professor, 生物分子応答研究センター, 助教授 (10183120)

Co-Investigator(Kenkyū-buntansha) NEMOTO Keisuke  The University of Tokyo, Asian Natural Environmental Science Center, Associate Professor, アジア生物資源環境研究センター, 助教授 (40211461)
YAMAMOTO Ryoichi  Tezukayama College, Food Sciences Course, Professor, 食品科学, 教授 (10047384)
TANIMOTO Eiichi  Nagoya City University, Institute of Natural Sciences, Professor, 自然科学研究教育センター, 教授 (90080283)
YAMAGUCHI Hiroshi  株式会社山電, 技術部・主任研究員
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1998: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsCreep mater / Windows computer / Gibberellin / Cell extension / Cell wall extensibility / Rice (Oryza) / dwarf mutant / イネ / 細胞壁 / 伸展曲線 / 葉鞘 / 稈 / エンドウ / ヘミセルロース
Research Abstract

We attempted to improve creep mater to estimate physical properties, which are major factors for plant morphogenesis. We also attempted to establish physical model to adapt cell wall loosening.
The mechanism of gibberellin (GA) -induced leaf sheath growth was examined using a dwarf mutant of rice (Oryza sativa L. cv. Tan-ginbozu) treated in advance with an inhibitor of GA biosynthesis. Gibberellic acid (GA) enhanced the growth of the second leaf sheath, but auxins did not. Measurement of the mitotic index and cell size revealed that cell elongation rather than cell division is promoted by GA. GA increased the extensibility of cell walls in the elongation zone of the leaf sheath. It also increased the total amount of osmotic solutes including sugars in the leaf sheath, but did not increase the osmotic concentration of the cell sap, due to accompanying increase in cell volume by water absorption. In the later stage of GA-induced growth, starch granules completely disappeared from leaf sheath cells, whereas dense granules remained in control plants. These findings indicate that GA enhances cell elongation by increasing wall extensibility, osmotic concentration being kept unchanged by starch degradation.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Ikeda, A.: "Charactrization and expression of GASR1, a root-specific GAST1-like protein in rice"Rice Genet. Newslett.. 14. 150-153 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C.: "The first leaf sheath growth promoted by gibberellins in dwarf mutant of rice"Rice Genet. Newslett.. 14. 153-155 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C.: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice"Planta. 205. 145-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C.: "Starch degradation during gibberellin-induced leaf sheath growth in rice"Rice Genet. Newslett.. 15. 114-117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamaguchi, J.: "Characterization of b-amylase and its deficiency in various rice cultivars"Theor. Appl. Genet.. 98. 32-38 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C.: "Tranverse vein differentiation associated with the gas space formation-cell fate of middle layer in leaf sheath development of rice"Ann. Bot.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanimoto, E.: "Measurement of viscoelatic properties of root cell walls affected by low pH in lateral roots of Pisum sativum L"Plant and Soil. (印刷中). (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. F. Ma: "A1 Binding in the epidermis cell wall inhibits cell elongation of okura hyprocotyl"Plant and Cell Physiol.. 40(5). 549-556 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyamoto, K.: "Auxin polar transport in Arabidopsis under simulated microgravity conditions relevance to growth and development"Adv. Space Res.. 23. 65-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii, S.: "effects of salinity on growth and content of intracellular solutes in Heterococcus sup. (Tribonematales, Xanthophyceae) form antarcita"Phycol. Res.. 47. 65-69 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松倉千昭: "植物の篩管輸送-スクローストランスポーターの発現と機能"化学と生物. 37. 639-642 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 谷本英一: "根の生長と細胞壁粘弾性変化-チャの場合"根の研究. 18)4. 162-163 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 根本圭介: "登熟期の稲の桿いおける(1-3,1-4)-βグルカナーゼ遺伝子の発現"日本作物学会記事. 68(別1号). 32-33 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ikeda, A., Yamaguchi, J. and Futsuhara, Y.: "Characterization and expression of GASR1, a root-specific GAST1-like protein in rice"Rice Genet. Newslett.. 14. 150-153 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C. and Yamaguchi, J.: "The first leaf sheath growth promoted by gibberellins in dwarf mutant of rice"Rice Genet. Newslett.. 14. 153-155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C., Itoh, S., Nemoto, K., Tanimoto, E. and Yamaguchi, J.: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice"Planta. 205. 145-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C. and Yamaguchi, J.: "Starch degradation during gibberellin-induced leaf sheath growth in rice"Rice Genet. Newslett.. 15. 114-117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamaguchi, J., Itoh, S., Saito, T., Ikeda, A., Tashiro, T. and Nagato, Y.: "Characterization of b-amylase and its deficiency in various rice cultivars"Theor. Appl. Genet.. 98. 32-38 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsukura, C., Kawai, M., Toyofuku, K., Barrero, RA., Uchimiya, H. and Yamaguchi, J.: "Transverse vein differentiation associated with the gas space formation - cell fate of middle layer in leaf sheath development of rice"Ann. Bot. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanimoto, E., Fujii, S., Yamamoto, R., Inanaga, S.: "Measurement of viscoelastic properties of root cell walls affected by low pH on lateral roots of Pisum sativum L."Plant and Soil. on press. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. F. Ma, R. Yamamoto, D. J. Nevins, H. Matsumoto and P. H. Brown: "A1 Binding in the epidermis cell wall inhibits cell elongation of okura hyprocotyl"Plant and cell Physiol. 40(5). 549-556 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miyamoto, K., M. Oka, R. Yamamoto, Y. Matsuda, T. Hoson, S. Kamisaka, J. Ueda: "Auxin polar transport in Arabidopsis under simulated microgravity conditions-relevance to growth and development"Adv. Space Res.. 23. 65-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujii, S., R. Yamamoto, S. Nakayama, S. Yasui and P.A. Broady: "Effects of salinity on growth and content of intracellular solutes in Heterococcus sup. (Tribonematales, Xanthophyceae) form antarcita"Phycol. Res. 47. 65-69 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ikeda,A.: "Charaterization and expression of GASR1,a root-specific GAST1-like protein in rice."Rice Genet. Newslett.. 14. 150-153 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsukura,C: "The first leaf sheath growth promoted by gibberellins in dwarf mutant of rice."Rice Genet. Newslett.. 14. 153-155 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsukura,C: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice."Planta. 205. 145-152 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsukura,C: "Starch degradation during gibberellin-induced leaf sheath growth in rice."Rice Genet. Newslett.. 15. 114-117 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamaguchi,J.: "Characterization of b-amylase and its deficiency in various rice cultivars."Theor. Appl. Genet.. 98. 32-38 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsukura,C: "Transverse vein differentiation associated with the gas space formation-cell fate of middle layer in leaf sheath development of rice-"Ann. Bot.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanimoto,E.: "Measurement of viscoelatic properties of root cell walls affected by low pH in lateral roots of Pisum sativum L."Plant and Soil. (印刷中). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J. F. Ma: "A1 Binding in the epidermis cell wall inhibits cell elongation of okura hyprocotyl."Plant and Cell Physiol.. 40(5). 549-556 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyamoto,K.: "Auxin polar transport in Arabidopsis under simulated microgravity conditions-relevance to growth and development."Adv. Space Res.. 23. 549-556 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujii,S.: "Effects of salinity on growth and content of intracellular solutes in Heterococcus sup. (Tribonematales,Xanthophyceae) form antarcita"Phycol. Res.. 47. 65-69 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松倉千昭: "植物の篩管輸送-スクローストランスポーターの発現と機能"化学と生物. 37. 639-642 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 谷本英一: "根の生長と細胞壁粘弾性変化-チャの場合"根の研究. (8)4. 162-163 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 根本圭介: "登熟期の稲の稈における(1-3,1-4)-β-グルカナーゼ遺伝子の発現"日本作物学会記事. 68(別1号). 32-33 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsukura C: "Leaf sheath growth promoted by gibberellins in dwarf mutant of rice." Planta. 205. 145-152 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Umemura T: "Sugar sensing and α-amylase gene repression in rice embryos." Planta. 204. 420-428 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morita A: "Functional dissection of a sugar-repressed α-amylase gene(RAmy1A)" FEBS Lett.423. 81-85 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toyofuku K: "Promoter elements required for sugar-repression of the RAmy3D GENE FOR α-amylase in rice." FEBS Lett.428. 275-280 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamaguchi J: "Characterization of β-amylase and its deficiency in various rice cultivars." Theor.Appl.Genet.98. 32-38 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 根本圭介: "登熟期の稲の桿における(1-3,1-4)-β-グルカナーゼ遺伝子の発現" 日本作物学会記事. 68(印刷中)(別号1).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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