| Project/Area Number |
10559017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | TOKYO METOROPOLITAN UNIVERSITY |
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Principal Investigator |
KOSHIDA Tomokazu Tokyo Metropolitan University, Dept. of Biol. Sci., 理学研究科, 助教授 (80117704)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Yuji Institute of Physical and Chem. Res., FRP, フロンティア, チームリーダ (30100051)
FURUYA Nobuhisa Tokyo Metropolitan University, Dept. of Biol. Sci., 理学研究科, 助手 (50244413)
KOMANO Teruya Tokyo Metropolitan University, Dept. of Biol. Sci., 理学研究科, 教授 (00087131)
OSIO Hiromu Sumitomo Chem. Ind., Inst. Bioengineering, 生命工学研究所, 所長
KAWAKAMI Naoto Meiji Univ., Faculty of Agric., 農学部, 専任講師 (10211179)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | abscisic acid / auxin / aldehyde oxidase / Arabidopsic thaliana / cDNA cloning / transgenic plant / 遺伝子組み換え植物 |
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| Research Abstract |
Aldehyde oxidase (AO) has been extensively investigated in animals and microorganisms. In plants, however, there was only a limited information on this enzyme. A great interest in this enzyme has been in its possible involvement in indole-3-acetic acid (IAA) and abscisic acid (ABA) biosynthesis. In the present study, we cloned four full-length AO cDNAs from Arabidopsis thaliana and investigate their physiological functions using transgenic plants with these genes. We also tried to develop these results to agricultural application.
(1) Four different putative AO cDNA clones were obtained from A. thaliana. Their products were analyzed by using antibodies each specific to four AOs in A. thaliana.
(2) The four AO cDNAs were introduced into yeast Pichia pastoris and the products were functionally expressed in the yeast cells. The result revealed that two AO genes encoded three AOs in A. thaliana seedlings and another AO gene produced a leaf AO. Biochemical studies using AOs expressed in yeast indicated that one of seedling AO might be involved in IAA biosynthesis and a leaf AO is a most possible candidate of an AO that catalyzes oxidation of abscisic aldehyde to form ABA.
(3) The four AO cDNA clones were introduced into A. thaliana and some over expressed and suppressed lines were obtained for each DNA construct.
(4) We also succeeded screening several knockout lines with these genes.
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