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Role of GPI anchor in plant cell wall construction

Research Project

Project/Area Number 10640623
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OKUYAMA Hidetoshi  Hokkaido Univ., Grad. Sch. Environ. Earth Sci., assoc. Prof., 大学院・地球環境科学研究科, 助教授 (90125295)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsSpirodela / purple acid phosphatase / GPI anchor / cell wall / Marchantia / desiccation related protein / secretory protein / apoplast / 復活草 / ウキクサ / cell wall / GPIアンカータンパク質 / シグナル伝達 / ホスファターゼ / 皮層細胞
Research Abstract

The Spirodela oligorrhiza purple acid phosphatase (PAP) inducibly synthesized under phosphate-deficient conditions was at the first time proved to be a glycosylphosphatidylinositol (GPI)-anchored protein found in higher plants. To know the primary structure of the GPI-anchored PAP its cDNA clone has been obtained by a combination of cDNA library screening and 5' rapid amplification of cDNA from plants (-P plants) grown under phosphate-deficient conditions. The open reading frame of the S.oligorrhiza PAP cDNA consists of 1365 bp encoding a 455-amino acid protein. Its deduced amino acid sequence shows 82% and 80% similarity with that of PAP from Arabidopsis thaliana and Phaseolus vulgaris, respectively. The amino acid residue Ala-439 followed by two more small amino acid residues, Asp and Ser, is predicted to be the GPI-anchoring ω-site. The absence of a dibasic motif upstream of the putativeω-site suggests that the PAP is a cell wall protein. This presumption is supported by the finding that PAP was released by digestion of the cell wall fraction with cellulase. The GPI anchor is speculated to be a signal for transporting PAP to the cell wall. Immuno-histochemical results using-P plant roots demonstrate that PAP is preferentially distributed in the outer-most cortical cells of roots but not in the epidermis, suggesting its role for acquiring inorganic phosphate under phosphate-deficient conditions.
Screening of new GPI-anchored proteins has beencarried out using cultured cells of the moss Marchantia polymorpha. Radio-labeled ethanolamine and mystic acid were both incorporated significantly into a 47 kDa protein which was the sole major secretory protein of the moss. The N-terminal amino acid sequencing of the protein indicates thatthe 47 kDa protein is a protein similar to the desiccation-related protein from Craterostigma plantagineum. The moss protein would be a new GPI-anchored protein.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 奥山英登志: "Glycosyl phosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha."Biochem. Soc.Transact.. 28. 589-590 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 一瀬信敏: "Gene cloning and expression of the catalase from the hydrogen-peroxide resistant bacterium Vibrio rumoiensis S-1 and its subcellular localization."J.Biosci.Bioeng. 90 No.5. 530-534 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] G.A.Thompson,Jr.: "Lipid-linked proteins of plants."J.Lipid Res.. 39. 19-39 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] U.Schweiger-Hufnagel: "Identification of the extracellular polysaccharide produced by the snow mold fungus Microdochium nivale."Biotech.Lett.. 22. 183-187 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 中里洋: "The GPI-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase."Plant Phiysiol.. 118. 1015-1020 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Okuyama, et al.: "Glycosyl phosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha."Biochem. Soc. Transact.. 28. 589-590 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Ichise, et al.: "Gene cloning and expression of the catalase from the hydrogen-peroxide resistant bacterium Vibrio rumoiensis S-1 and its subcellular localization."J.Biosci.Bioeng. 90-5. 530-534 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] G.A.Thompson, Jr. and H.Okuyama: "Lipid-linked proteins of plants."J.Lipid Res.. 39. 19-39 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] U.Schweiger-Hufnagel, et al.: "Identification of the extracellular polysaccharide produced by the snow mold fungus Microdochium nivale."Biotech.Lett.. 22. 183-187 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Nakazato, et al.: "The GPI-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase."Plant Phiysiol.. 118. 1015-1020 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 奥山英登志: "Glycosyl phosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha."Biochemical Society Transactions. 28. 589-590 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 一瀬信敏: "Gene cloning and expression of the catalase from the hydrogen-peroxide resistant bacterium Vibrio rumoiensis S-1 and its subcellular localization."J.Biosci.Bioeng. 90 No.5. 530-534 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] G.A.Thompson, Jr.: "Lipid-linked proteins of plants."J.Lipid Res.. 39. 19-39 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 奥山英登志 他: "Identification of the extracellular polysaccharide produced by the snow mold fungus Microdochium nivale"Biotech. Lett.. 22. 183-187 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] G.A.Thompson Jr.and H.Okuyama: "Lipid-linked Proteins of Plants"J. Lipid Res.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Okuyama 他: "The Glycosylphosphatidylinositol-anchored phosphatase from Spirodela oligorrhiza is a purple and phosphatase" Plant Physiology. 118. 1015-1020 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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