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Specific binding between nuclear matrix and matrix attachment region DNA in rice.

Research Project

Project/Area Number 10640625
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionUniversity of Tsukuba

Principal Investigator

NOMURA Koji  Institute of Agriculture and Forestry, University of Tsukuba Associate Professor, 農林学系, 助教授 (00183905)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordscell nucleus / nuclear matrix / chromatin / MAR / higher plants / rice / (1)細胞核 / (2)核マトリックス / (3)クロマチン / (4)高等植物 / (5)イネ
Research Abstract

Genomic DNA regions which bind to the nuclear matrix are called matrix attachment regions (MARs). In spite of their importance in transcription and duplication of the genome, little is known about the nature of MARs and MAR-binding proteins. I tried to reveal the nature of MARs and the binding proteins in this project research. Using rice cultured cells, I prepared nuclear matrices and cloned MARs from them. Sequences of those MARs were abundant with stretches of CAAAAA and TTTTTG.The sequences were known to bent double strand DNA molecules. It seemed that certain bendings of DNA molecules were important in specific binding between MARs and nuclear matrices. The next step of this research, therefore, was construction of three dimensional models of MARs to reveal the special characteristics of all of the MARs available in databases. According to a reported data, I and a graduate student prepared the three dimensional models of MARs. From the models, it was revealed that the repeating of A or T appeared as clusters in MARs. Also, MARs and transposons bent more than genes, promoters and enhancers.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Yamamura,Jun & NOMURA,koji: "Analysis of sequence-dependent curvature in matrix attachment regions."FEBS Letters. 489. 166-170 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamamura, Jun and NOMURA,Koji: "Analysis of sequence-dependent curvature in matrix attachment regions."FEBS Letters. 489. 166-170 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Jun YAMAMURA and Koji NOMURA: "Analysis of sequence-dependent curvature in matrix attachment regions."FEBS Letters. 489. 166-170 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Tamura: "Identification of Restriction Fragment Length Polymorphism Markers Tightly linked to a Major Photo period Sensitivity Gene, Se-1, and to a Blast Resistance Gene, Pi-Z^t, in Rice"SABRAO Journal of Breeding & Genetics. 30(2). 61-67 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Nomura: "Physiological and Morphological Aspects of Somatic Embryogenesis in W-Y,Soh and S,S,Bhojwari (eds) `Morphogenesis in Plant Tissue Cultures´"Kluwer Acadademic Publishers. 115-131 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 駒嶺 穆・野村港二 編著: "植物細胞工学入門" 学会出版センター, 336 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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