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Studies on the acidic wall proteins that activate the wall glucanases in maize coleoptiles.

Research Project

Project/Area Number 10640635
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionEhime University

Principal Investigator

INOUHE Masahiro  Ehime University, Faculty of Science, Associate Professor, 理学部, 助教授 (80203256)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsAuxin / Chitinases / Glucanases / Cellelongation / Cellwallmetabclison / Acidic wall protein / 細胞壁蛋白質 / 細胞壁グルカナーゼ / グルカン代謝
Research Abstract

Exo- and endoglucanases purified from the cell walls of maize coleoptiles mediate a specific degradation of cell wall β-(1,3)(1,4)-glucans. We now suggest that there is a possible role for apoplastic chitinase in the cell wall glucan-glucanase interaction resulting in an acceleration of these reactions. The acidic wall protein fraction which had been isolated from maize coleoptiles is capable of endochitinase activity. Moreover, this purified maize acidic chitinase strongly stimulates both exo- and endoglucanases in catalyzing the hydrolysis of β-(1,3)(1,4)-glucans. Selected chitinases from other sources exhibited similar behavior in β-glucan hydrolysis in the presence of glucanases. However, we found no evidence to suggest that chitinases alone were responsible of significant hydrolysis of β-glucans in the absence of glucanases. These results suggest that the wall chitinase(s) may cause small but significant changes in either the glucanase glycopeptide structure or that it might act to modify certain conformational elements of the glucan structure.
In cereal plants, auxin stimulates cell elongation, a process that is closely tied to metabolic modification of cell wall glucans. The precise nature of the chitinase interaction with the cell wall structural changes mediated by glucanases and the relationship to auxin action will be the focus of future experiments.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] M.Inouhe: "Peptide characteristics and immunological properties of exo-and endoglucanases purified from maize coleoptile cell walls."Journal of Plant Physiology. 154. 334-340 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Exo-and endoglucanases of maize coleoptile cell walls : their interaction and possbile regulation."International Journal of Biological Macromolecules. 27(2). 157-162 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Cell wall autolytic activities and distribution of cell wall glucanases in Zea mays L. seedlings."International Journal of Biological Macromolecules. 27(2). 151-156 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] B.R.Thomas: "Endo-1,3;1,4-β-glucanase from coleoptiles of rice and maize : role in the regulation of plant growth."International Journal of Biological Macromolecules. 27(2). 145-149 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Maruoka: "Interaction between maize wall glucanases and chitinases in the cell wall glucan hydrolysis."Plant Cell Physiol.. 41. S191 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Maruoka: "Effects of maize wall glucanases and chitinases in the various hydrolases activities of cell wall polysaccharides."Plant Cell Physiol.. 42(発表予定)(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Changes in the activities and polypeptide levels of exo- and endoglucanases in cell walls during developmental growth of Zea mays coleoptiles."Plant Cell Physiology. 39. 762-768 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] B.Thomas: "Malze coleoptlle endoglucanase is encoded by a novel gene family (accession no. AF072326)(PGR98-143)."Plant Physiology. 117. 1525 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Changes in the activities and polypeptide levels of exo- and endoglucanases in cell walls during developmental growth of Zea mays coleoptiles."Plant Cell Physiol.. 39. 762-768 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] B.R.Thomas: "Maize coleoptile endoglucanase is encoded by a novel gene family (accession no. AF072326)(PGR 98-143)."Plant Physiol.. 117. 1525 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Peptide characteristics and immunological properties of exo and endoglucanases purified from maize coleoptile cell walls."J.Plant Physiol.. 154. 334-340 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Maruoka: "Interaction between maize wall glucanases and chitinases in the cell wall glucan hydrolysis."Plant Cell Physiol.. 41. s191 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] B.R.Thomas: "Endo- 1,3 ; 1,4-β-glucanase from coleoptiles of rice and maize : role in the regulation of plant growth."Int.J.Biol.Macromol.. 27(2). 145-149 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Cell wall autolytic activities and distribution of cell wall glucanases in Zea mays L.seedlings."Int.J.Biol.Macromol.. 27(2). 151-156 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Exo- and endoglucanases of maize coleoptile cell walls : their interaction and possible regulation."Int.J.Biol.Macromol.. 27(2). 157-162 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Maruoka: "Effects of maize wall glucanases and chitinases on the various hydrolases activities of cell wall polysaccharides."Plant Cell Physiol.. 42(supplement)(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Inouhe: "Peptide characteristics and immunological properties of exo-and endoglucanases purified from maize coleoptile cell walls."Journal of Plant Physiology. 154. 334-340 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Inouhe: "Exo-and endoglucanases of maize coleoptile cell walls : their interaction and possbile regulation."International Journal of Biological Macromolecules. 27(2). 157-162 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Inouhe: "Cell wall autolytic activities and distribution of cell wall glucanases in Zea mays L.seedlings."International Journal of Biological Macromelecules. 27(2). 151-156 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] B.R.Thomas: "Endo-1,3;1,4-β-glucanase from coleoptiles of rice and maize : role in the regulation of plant growth."International Journal of Biological Macromolecules. 27(2). 145-149 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Maruoka: "Interaction between maize wall glucanases and chitinases in the cell wall glucan hydrolysis."Plant Cell Physiol.. 41. S191 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Maruoka: "Effects of maize wall glucanases and chitinases on the various hydrolases activities of cell wall polysaccharides."Plant Cell Physiol.. 42(印刷中)(発表予定).

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Inouhe: "Peptide characteristics and immunological properties of exo-and endoglucanases purified from maize coleoptile cell walls."Journal of Plant Physiology. 154. 334-340 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Inouhe: "Exo-and endoglucanases of maize coleoptile cell walls:their interaction and possbile regulation."International Journal of Biological Macromolecules. (発表予定).

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Inouhe: "Cell wall autolytic activities and distribution of cell wall glucanases in Zea mays L.seedlings."International Journal of Biological Macromolecules. (発表予定).

    • Related Report
      1999 Annual Research Report
  • [Publications] B.R.Thomas: "Endo-1,3:1,4-β-glucanase from coleoptiles of rice and maize:role in the regulation of plant growth."International Journal of Biological Macromolecules. (発表予定).

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Inouhe: "Changes in the activities and polypeptide levels of exo- and endoglucanases in cell walls during developmental growth of Zea mays coleoptiles." Plant Cell Physiology. 39. 762-768 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] B.Thomas: "Maize coleoptile endoglucanase is encoded by a novel gene family(accession no.AF072326)(PGR98-143)." Plant Physiology. 117. 1525 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Inouhe: "Peptide characteristics and immuno-logical properties of exo- and endoglucanases purified from maize coleoptile cell walls." Journal of Plant Physiology. 発売予定.

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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