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Genetic engineering of salt tolerant plants

Research Project

Project/Area Number 10640641
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionMeijo University

Principal Investigator

TAKABE<TERUHIRO  Meijo University, Fac. of Science & Technology, Professor, 総合研究所, 教授 (80097766)

Co-Investigator(Kenkyū-buntansha) 日比野 隆  名城大学, 理工学部, 講師 (70218741)
TANAKA Yoshito  Meijo University, Fac. of Science & Technology, Lecturer, 理工学部, 講師 (10247679)
ISHIKAWA Hiroshi  Meijo University, Fac. of Science & Technology, Associate Prof., 理工学部, 助教授 (40076592)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordssalt stress / photosynthesis / stress tolerant plant / stress response / crop yield / 形質転換植物 / 乾燥ストレス
Research Abstract

Salinity is one of the important limiting factors in crop productivity and mach effort has been made to understand salt tolerance in plants. Our objects are to constract salt tolerant plant by using the genetic engineering technique. In this project, we obtained the following results.
1) A freshwater Synechococcus PCC 7942 was transformed with Vibrio NaィイD1+ィエD1/HィイD1+ィエD1 antiporter. They showed the tolerance to LiCl, but not to NaCl in Synechococcus cells.
2) The potential of SOD in the protection against salt stress was examined using transgenic rice plants. The yeast mitochondrial Mn-SOD was expressed in rice chloroplasts. The levels of ascorbate peroxidase increased upon the overexpression of Mn-SOD which caused the tolerance to salt stress in rice.
3) The dnaK1 gene of a halotolerant cyanobacterium A. halophytica has a long C-terminal region rich in acidic amino acid residues. It was found that the extra C-terminal region of A. halophytica DnaK1 plays an important role in the refolding of other denatured proteins at high salinity.
4) To explore the potential role of catalase and glycine betaine in the protection of cyanobacteria from damage due to salt stress, we transformed a freshwater cyanobacterium Synechococcus sp. PCC 7942 with shuttle vectors that contained the E. coli katE, bet, and katE plus bet (katE/bet) genes. The potential role of photorespiration in the protection against salt stress was examined using transgenic rice plants in which rice was transformed with a chloroplastic glutamine synthetase gene from rice. To test the role of the heat shock protein DnaK/Hsp70 in salt tolerance, we made transgenic plants of Nicotina tabacum with DnaK1 from a halotolerant cyanobacterium Aphanothece halophytica.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Nomura/Hibino/Takabe/Sugiyama/Yokota/Miyake/Takabe: "Transgenically produced glycine betaine protects ribulose 1,5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp.PCC7942 under salt stress"Plant Cell Physiol.. 39. 425-432 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaku/Hibino/Tanaka/Takabe/Nakamura/Takabe: "Expression of nhaAv gene encoding Na^+/H^+ antiporter from Vibrio alginolyticus in a freshwater cyanobacterium Synechococcus sp.PCC7942 confer lithium tolerance, but not sodium tolerance"Plant Cell Physiol.. 40. 557-564 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka/Hibino/Hayashi/Tanaka他: "Salt tolerance of transgenic rice overexpression yeast MnSOD"Plant Science. 148. 131-138 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugino/Hibino/Niil/Takabe/Takabe: "Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica aquires resistance to salt stress in transgenic tobacco plants"Plant Science. 146. 81-88 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang/Meng/Ishikawa/Hibino/Tanaka/Nii/Takabe: "Photosynthetic adaptation to salt stress in three-color leaves of a C4 plant Amaranthus tricolor"Plant Cell Physiol.. 40. 668-674 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hibino/Kaku/Yoshikawa/Takabe/Takabe: "Molecular characterization of DnaK from a halotolerant cyanobacterium Aphanothece halophytica for ATPase, protein folding, and copper binding under various salinity conditions"Plant Molecular Biology. 40. 409-418 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takabe/Nakamura/Nomura/Hayashi他: "Stress Responses of Photosynthetic Organisms"Elsevier Science. 115-131 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 高部/田中/日比野: "光がもたらす生命・地球共進化"弘文社. 202-208 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nomura/Hibino/Takabe/sSugiyama/Yokota/Miyake/Takabe: "Transgenically produced glycine betaine protects ribulos 1,5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp. PCC7942 under salt stress"Plant Cell Physiol. Vol39,No.1. 425-432 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kaku/Hibino/Tanaka/Takabe/Nakamura/Takabe: "Experession of nhaAv gene cncoding NaィイD1+ィエD1/HィイD1+ィエD1antiporter from Vibrio alginolyticus in a freshwater cyanobacterium Synechococcus sp.PCC7942 confer lithium tolerance, but not sodium tolerance"Plant Cell Physiol.. Vol.40,No.1. 557-564 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka/Hibino/Hayashi/Tanaka et al.: "Salt tolerance of transgenic rice overexpression yeast MnSOD"Plant Science. Vol.48. 131-138 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sugino/Hibino/Nii/Takabe/Takabe: "Overexpression of Dnak from a halotolerant cyanobacterium Aphanothece halophytica aquires resistance to salt stress in transgenic tobacco plants"Plant Science. Vol. 46. 81-88 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang/Meng/Ishikawa/Hibino/Tanaka/Nii/Takabe: "Photosynthetic adaptation to salt stress in three-color leaves of a C4 plant Amaranthus tricolor"Plant Cell Physiol.. Vol.40,No.1. 668-674 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hibino/Kaku/Yoshikawa/Takabe/Takabe: "Molecular characterization of DnaK from a halotolerant cyanobacterium Aphanothece halophytica for ATPase, protein folding, and copper binding under various salinity conditions"Plant Molecular Biology. Vol.40,No.1. 409-418 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanaka/Nakamura/Nomura/Hayashi et al.: "Stress Responses of Photosyntheitic Oragnisms"Elsevier Science. 115-131 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takabe/Tanaka/Hibino: "Coevolution of biology and earth by sun light"Kobunsha. 202-208 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nomura/Hobino/Takabe/Sugiyama/Yokota/Miyake/Takabe: "Transgenically produced glycine betaine protects ribulose 1,5-bisphosphate carboxylase/oxygenase from inactivation in Synechococcus sp. PCC7972 under salt stress"Plant Cell Physiol.. 39. 425-432 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kaku/Hibino/Tanaka/Takabe/Nakamura/Takabe: "Expression of nhaAv gene encoding Na+/H+ antiporter from Vibrio alginolyticus in a freshwater cyanobacterium Synechococcus sp. PCC 7942 confer lithium tolerance, but not sodium tolerance"Plant Cell Physiol.. 40. 557-564 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka/Hibino/Hayashi/Tanaka他: "Salt tolerance of transgenic rice overexpression yeast MnSOD"Plant Science. 148. 131-138 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugino/Hibino/Nii/Takabe/Takabe: "Overexpression of DnaK from a holotolerant cyanobacterium Aphanothece halophytica aquires resistance to salt stress in transgenic tobacco plants"Plant Science. 146. 81-88 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wang/Meng/Ishikawa/Hibino/Tanaka/Nii/Takabe: "Photosynthetic adaptation to salt stress in three-color leaves of a C4 plant Amaranthus tricolor"Plant Cell Physiol.. 40. 668-674 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hibino/Kaku/Yoshikawa/Takabe/Takabe: "Molecular characterization of DnaK from a holotolerant cyanobacterium Aphanothece halophytica for ATPase, protein folding, and copper binding under various salinity conditions"Plant Molecular Biology. 40. 409-418 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takabe/Nakamura/Nomura/Hayashi他: "Stress Responses of Photosynthetic Organisms"Elsevier Science. 115-131 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 高倍/田中/日比野: "光がもたらす生命・地球共進化"弘文社. 202-208 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nomura, Hibino, Takabe 他: "Transgenically produced glycine betaine profects ribulose 1,5-bisphosphate carboxy genase/oxygenase from inactivation in Synecho coccus sp.…" Plant Cell Physiol.39. 425-432 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hibino, Kaku, …, Takabe: "Molecular charucterization of Dnak from a halotolerant…" Plant Mol.Biol.in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kaku, Hibino, Tanaka, Takabe: "Expression of nhaAv gene enceding Na^<+1>/H^<+1> antiporter…" Plant Cell Physiol.in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mi, Endo, Ye, Takabe 他: "Stromal NADH-dehydrogenase functions as a mediator…" Photosynthesis Research. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka, Katada, Takabe 他: "Electron flow from NAD (P) H dehydrogenase to photosystem I…" Plant Cell Physiol.38. 1311-1318 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirota, Endo, Takabe 他: "Interactions of Cytochrome c and cytochrome f with…" J.Amer.Chem.Soc.121. 849-855 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takabe, Nakamura 他: "Stress Responses of Photosythetic Organisms" Elsevier Science, 115-131 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 高倍,田中,日比野: "光がもたらす生命・地球共進化" 弘文社, (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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