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MORPHOLOGICAL IDENTIFICATION OF SALT SENSITIVE EPITHELIAL CELL IN THE TOAD SKIN

Research Project

Project/Area Number 10640656
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionTeikyo University

Principal Investigator

NAGAI Takatsohi  TEIKYO UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PHYSIOLOGY, LECTURER, 医学部, 講師 (50130026)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Hiroaki  SHIZUOKA UNIVERSITY, FACULTY OF SCIENCE, ASSOCIATE PROFESSOR, 理学部, 助教授 (90216854)
KOYAMA Hiromichi  YOKOHAMA CITY UNIVERSITY, COLLEGE OF NURSING, ASSOCIATE PROFESSOR, 看護短期大学部, 助教授 (00170407)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordstoad / skin epithelium / chemoreception / sodium channel / fluorescent dye / electron microscope / antibody / spinal nerve / カエル / カーリウムチャネル / アミロライド
Research Abstract

Our previous project showed chemosensory function of the ventral skin in desert toads (Bufo alvarius) by combined approaches in behavior, physiology and anatomy. We reported putative chemosensory cells in the toad skin. In the present project we extended anatomical approaches to associate the structure of chemosensory cells with proposed sensory transduction in the skin. Those cells labeled with fluorescencent dye were photoconverted in the presence of DAB for electron microscopic examination. Cytological features of the cells were neither reminiscent of Merkel cells and flask cells, but rather similar to those of surrounding non-specialized epithelial cells. Such cells were distributed in the germinativum and spinosum layers, but none of them were seen in the outermost layer of living cells, i.e., the granulosum layer. Some of those cells had close contact with spinal nerve endings. However, synapses were not revealed in the contact area because of limited quality in the present fixat … More ion protocol for electron microscopy. These findings suggest that stimulants such as Na^+ ions need to flow through epithelial cell layers to putative chemosensory cells and/or nerve endings to induce neural excitation. The antibody was generated against peptide of alpha subunit of epithelial Na^+ channels (ENaC) in renal tissues of Xenopus. The antibody reacted positively to the apical cell membrane of granulosum cells. Therefore, Na^+ ions possibly flow into the cell through ENaC in the membrane and are pumped out through the basal membrane. Previous physiological experiments showed that the spinal nerve responses were reduced by amiloride, a specific blocker for ENaC. However, the reduction was only partial. Residual neural responses were probably mediated by the ions passing through intercellular spaces in the granulosum layer, because the spinal nerve responses were also reduced by lanthanum and because occulusion of the intercellular spaces with precipitates of lanthanum was revealed by subsequent histological examination. Less

Report

(4 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Nii, D.Takeuchi, H.-A., Nagai, T.: "Amiloride-sensitive sodium channels in taste organ of metamorphosing urodeles"Zool.Sci.. 15(Suppl.). 102 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Koyama, H., Hoff, K.vS., Hillyard., S.D.: "Desert toads discriminate salt taste with chemosensory function of the ventral skin"J.Comp.Neurol.. 408. 125-136 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Koyama, H., Hillyard, S.D.: "The epithelial cells with spinal innervation in the chemosensory ventral skin of desert toads"Zool.Sci.. 16(Suppl). 99 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Hillyard, S.D.: "Chemosensory function of the spinal nerves that innervate the ventral skin in desert toads"Jpn.J.Physiol.. 49(Suppl.). 155 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Takeuchi, H.-A.: "Epithelial Na^+ channels to mediate salt taste transduction in metamorphosed salamanders"Jpn.J.Physiol. 50(Suppl.). 138 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Torii, Y, Kanazashi, K., Takeuchi, H.-A., Kasai, M., Takada, M., Nagai, T.: "Immunohistochemical study of amiloride-sensitive Na^+ channel in the skin of anuran amphibian"Zool.Sci.. 17(Suppl.). 93 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 長井孝紀, 小山洋道, 竹内浩昭, Stanley D.HiUyard: "皮膚上皮内部に分布する塩味受容器と推定される細胞について"日本味と匂学会誌. 7(3). 351-354 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hillyard, S.D., Hoff, K., Nagai, T.: "Chemosensory function of the amphibian skin : transcellular and paracellular mechanisms"日本味と匂学会誌. 8(3). 289-290 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Koyama, H., Takeuchi, H.-A, Hillyard, S.D.: "Putative salt receptor cells in the epidermal cell layers of desert toads, Bufo alvarius"Chem.Senses. 26(3). 297 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Koyama, H., Torii, Y., Takeuchi, H.-A., Hillyard, S.D.: "Transcellular and paracellular pathways for salt taste transduction in the ventral skin of desert toads"Chem.Senses. 26(6). 762 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koyama, H., Nagai, T., Takeuchi, H.-A., Hillyard., S.D.: "The spinal nerves innervate putative chemosensory cells in the ventral skin of desert toads Bufo alvarius"Cell Tissue Res.. 304. 185-192 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Nii, D., Takeuchi, H.-A.: "Amiloride blocks salt taste transduction of the glossopharyngeal nerve in metamorphosed salamanders"Chem.Senses. 26(8). 965-969 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai, T., Takada, M., Takeuchi, H.-A., Koyama, H., Hillyard, S.D.: "Sodium transport and chemosensory function in the amphibian skin"Jpn.J.Physiol. 51(Suppl.). 59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hillyard, S.D., Hoff, K., Nagai, T.: "Chemosensory function of the amphibian skin : transcellular and paracellular mechanisms"Chem.Senses. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nil D. Takeuchi H.-A. and Nagai T.: "Amiloride-sensitive sodium channels in taste organ of metamorphosing urodeles."Zool. Sci.. 15(Suppl.). S102 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T., Koyama H., Hoff K. vS., and Hillyard S. D.: "Desert toads discriminate salt taste with chemosensory function of the ventral skin"J. Comp. Neurol.. 408. 125-136 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T., Koyama H., and Hillyard S. D.: "The epithelial cells with spinal innervation in the chemosensory ventral skin of desert toads"Zool. Sci.. 16 (Suppl.). S99 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T. and Hillyard S. D.: "Chemosensory function of the spinal nerves that innervate the ventral skin in desert toads"Jpn. J. Physiol.. 49 (Suppl.). S155 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T. and Takeuchi H.-A.: "Epithelial Na+ channels to mediate salt taste transduction in metamorphosed salamanders"Jpn. J. Physiol.. 50 (Suppl.). S138 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Torii Y, Kanazashi K., Takeuchi H.-A., Kasai M., Takada M. and Nagai T.: "Immunohistochemical study of amiloride-sensitive Na^+ channel in the skin of anuran amphibian"Zool. Sci.. 17 (Suppl.). S93 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T., Koyama H., Takeuchi A., Sanley D. Hillyard: (The Japanese Journal of Taste and Smell Research). 7(3). 351-354 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hillyard S. D., Hoff K. and Nagai T.: "Chemosensory function of the amphibian skin : transcellular and paracellular mechanisms"(The Japanese Journal of Taste and Smell Research). 8(3). 289-290 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T., Koyama H., Takeuchi H.-A. and Hillyard S. D.: "Putative salt receptor cells in the epidermal cell layers of desert toads, Bufo alvarius"Chem. Sensed. 26(3). S297 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T., Koyama H., Torii Y., Takeuchi H. -A. and Hillyard S. D.: "Transcellular and paracellular pathways for salt taste transduction in the ventral skin of desert toads"Chem. Sensed. 26(6). S762 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koyama H., Nagai T, Takeuchi H.-A., and Hillyard S.D.: "The spinal nerves innervate putative chemosensory cells in the ventral skin of desert toads Bufo alvarius"Cell Tissue Res.. 304. 185-192 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagai T, Nii D. and Takeuchi H.-A.: "Amiloride blocks salt taste transduction of the glossopharyngeal nerve in metamorphosed salamanders"Chem. Senses. 26(8). 965-969 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] agai T, Takada M., Takeuchi H.-A., Koyama H. and Hillyard S. D.: "Sodium transport and chemosensory function in the amphibian skin"Jpn. J. Physiol.. 51 (Suppl.). S59 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hillyard S. D., Hoff K. and Nagai T.: "Chemosensory function of the amphibian skin : transcellular and paracellular mechanisms"Chem. Senses.. 27(3) (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Koyama,H.,Nagai,T.,Takeuchi,H.-A.,and Hillyard.,S.D.: "The spinal nerves innervate putative chemosensory cells in the ventral skin of Desert toads Bufo alvarius.."Cell Tissue Res. In press 2001. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagai.T.,Koyama.H.,Torii.Y.,Takeuchi.H.-A.,and Hillyard.S.D.: "Transcellular and paracellular pathways for salt taste transduction in the ventral skin of desert toads."Abstr.13th Int.Sympo.Olfaction and Taste.. 147-148 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 長井孝紀,小山洋道,竹内浩昭,S.D.Hillyard: "皮膚上皮内部に分布する塩味受容器と推定される細胞について"日本味と匂学会誌. 7(3). 351-354 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Torii.Y.,Kanazashi.K.,Takeuchi.H.-A.,Kasai,M.,Takada,M.,and Nagai,T.: "Immunohistochemical study of amiloride-sensitive Na^+ channel in the skin of anuran amphibian."Zool.Sci. in press. 17(Suppl.). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagai,T.and Takeuchi,H.-A.: "Epithelial Na^+ channels to mediate salt taste transduction in metamorphosed salamanders."Jpn.J.Physiol. in press. Suppl.. (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagai,T.,Koyama,H.,Hoff,K.vS.,and Hillyard.,S.D.: "Desert toads discriminate salt taste with chemosensory function of the ventral skin."J.Comp.Neurol.. 408. 125-136 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagai,T.,Koyama,H.,and Hillyard,S.D.: "The epithelial cells with spinal innervation in the chemosensory ventral skin of desert toads"Zool.Sci.. 16(Suppl.). 99S (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagai,T.and Hillyard,S.D.: "Chemosensory function of the spinal nerves that innervate the ventral skin in desert toads"Jpn.J.Physiol.. Suppl(inpress). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagai,T.,Koyama,H.,Hoff,K.vS.,and Hillyard.,S.D.: "Desert toads discriminate salt taste with chemosensory function of the ventral skin." Journal of Comparative Neurology. in press (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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