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Molecular neuro-physiological study of insect diapause mechanism

Research Project

Project/Area Number 10640665
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SHIMIZU Isamu  Center for Ecological Research, Kyoto University, Professor, 生態学研究センター, 教授 (80025486)

Co-Investigator(Kenkyū-buntansha) IWASA Taturo  Himeji Techical University, Department of Science, Associate Professor, 理学部, 助教授 (00133926)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPhotoperialic receptor / Bombyx mori / brain / opsin / Visual Pigment / subcloning / eye / Boceropsin / DNA / 単眼 / PCR
Research Abstract

PCR was carried out using cDNA prepared from brain of the silkworm, Bombyx mori with primers by which opsin encoding genes of the compound eyes can be amplified. We got an amplification of a band corresponding about 200 bases, and then subcloned a fragment of 210 bases DNA (70 amino acids) encoding a opsin-like gene. On the base of this DNA sequence, we conducted 3'-5'-Race method to determine all length of ORF region. The cloned DNA sequence had about 1600 bases and the ORF coded 377 amino acids. It was found that the DNA sequence has a high homology with ManipI reported as one of the photoreceptive pigments in the Manduca sexta compound eyes. We named this opsin like protein in the silkworm brain Boceropisn (bombyx cerebral opsin). One peptide corresponding to the carboxyterminal 25 amino acids of Boceropsin was synthesized. And immunocytochemistry revealed that some pairs of cells of the silkworm brain showed potent immunoreactive to the rabbit antisera of the peptide. These immunoreactive cells is suggested to be a pupative photoreceptor involved in the photoperiodic response in the silkworm.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 清水 勇: "カイコ脳に発現するオプシン様蛋白質Boceropsin"日本時間生物学会誌. 5巻2号. 59 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I.Shimizu: "Clonning and Characterization of CDNA encoding per from Apiscerana"Zoological Science. 16巻. 101 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Iwasa: "G protein alpha submit genes in Octopus photoneceptor cells"Zoological Science. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 清水 勇: "地球生態系と光環境"「光環境と生物の進化」共立出版社. 30-43 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I. Shimizu: "Boceropsin : opsin like protein expressed in the silkworm brain."Journal of Japanese Society for Chronobiology. 5. 59 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I. Shimizu: "Clonning and characterization of cDNA encoding per from Apis cerana."Zoological Science. 16. 101 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Iwasa: "G protein alphasubunit genes in octopus photoreceptor cells"Zoological Science. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] I. Shimizu: "Global ecosystem and light environments"Photoenvironments and Evolution (Kyoritsu Publication). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 清水勇、山川義徳、岩佐達郎: "カイコ脳で発現するオプシン様蛋白質Boceropsin"日本時間生物学会誌. 5巻2号. 59 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I.Shimizu,: "Clonning and Characterization of cDNA encoding per from Apiscerana"Zoological Science. 16巻. 101 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Iwasa: "GProtein alpha subunit genes in octopus photoreoqtor cells"Zoological Science. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 清水 勇: "地球生態系と光環境"「光環境と生物の進化」共立出版社. 30-43 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimizu I.,Yamakawa,Y.,Minamoto,T.,Sakamoto,K.: "Cloning of genes encoding the Visucl Pigments in the Silkworm,Bombyx mori." Appl.Entomol.Tool. 33(1). 199-204 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwasa,T.,Colmenares,T.,Tsuda.M: "NMR and VV-VIS absorption spectroscopic studies on fluorinated octopus rhodopsin and its photo Products" J.Phys.Chem.102. 5602-5610 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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