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Force controlled atomic force microscopy for biological specimen.

Research Project

Project/Area Number 10650030
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 表面界面物性
Research InstitutionKinki University

Principal Investigator

MASTUMOTO Toshiro  Department of Intelligent mechanics, Faculty of Biology-Oriented Science and technology, Kinki University, Associate Professor, 生物理工学部, 助教授 (50110242)

Co-Investigator(Kenkyū-buntansha) KATO Nobuhiro  Department of Intelligent mechanics, Faculty of Biology-Oriented Science and technology, Kinki University, Research Associate, 生物理工学部, 助手 (60309268)
KIKUTA Hisao  Graduate school of engineering, Osaka prefecture university, Lecturer, 工学部, 講師 (10214743)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsForce control / Polyimide / Cantilever / Q factor / Hydroxyapatite / Biological compatibility / Ti-6Al-4V / 原子間力顕微鏡 / 生体親和性 / 表面粗さ / 骨芽細胞 / フォース・フィードバック / 制御 / 力平衡 / 生体 / 静電アクチュエータ / 遮断周波数 / 光ファイバ / 生物体
Research Abstract

Feasibility of polyimide cantilever for force feedback method is studied. The stability of the force feedback system is mainly improved by small Q factor and the low resonant frequency of the cantilever. The polyimide cantilever is fabricated and investigated its mechanical feature, especially Q factor in a vacuum. Form these values the required bandwidth of the force feedback system is estimated. The polyimide cantilever reduces the frequency requirement to 1/20, compared with the SiN cantilever with same feature except for internal damping. The system stability is confirmed by numerical simulations. The bandwidth shortage results in large oscillation of the force curve. To increase biological compatibility of Ti-6Al-4V, thin film of hydroxyapatite (HAp) was deposited on the polished substrate(0.1〜3.0μmR_<max>) by ArF excimer laser in an atmosphere of O_2+H_2O.The gas pressure was kept at 13.3Pa (1×10^<-1>Torr) during the deposition. The substrate temperature was varied torn 500℃ to 5 … More 60℃. Crystal stractures of HAp film were evaluated by X-ray diffraction (XRD). At temperatures less than 500℃ the films resulted in amorphous. At temperatures greater than 560℃ the films had mixture phase of crystallized HAp and tricalcium phosphate (TCP). Surface morphology of HAp film was observed by atomic-force microscope (AFM). Mechanical properties of film were measured by the scratch test and the peeling off test by using tensile test machine. The following results were obtained : (1) The crystallized HAp was obtained at about 530℃, (2) In the scratch test, HAp film was clearly removed at the load of 18N, (3) In the peeling off test, the adhesive strength of HAp film on the substratewere about 2.3, 5.4 and 10MPa for the surface roughness 0.1, 1.0 and 3.0μmRmax, respectively and (4) Biocompatibility of HAp film was evaluated by in-vitro. Unfortunately, to investigate biological specimen the stability of the force control atomic force microscopy system is not sufficient. Farther improvement of the force control system is required. Less

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Nobuhiro,Kato: "Dynamic model on force feedback system for nano-newton force measurement"Proc.16^<th> Int.Conf.on Force, Mass and Torque Measurements in Parallel with APMF'98. 338-345 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshiro,Matsumoto: "Formation of Biocompatible Hydroxyapatite Thin Films deposited by Laser Ablation"Bioceramics. 12. 499-502 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhiro,Kato: "System analysis of the force-feedback method for force curve measurements"Rev.Sci.Instrum. 70. 2402-2407 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhiro,Kato: "Polymer micro cantilevers for force control atomic force microscopy"Proceedings of the 5^<th> Asia-Pacific Symposium on Measurement of Force, mass and Torque(APMF2000). 91-96 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhiro Kato, Toshiro Matsumoto, Hisao Kikuta and Koichi Iwata: "Dynamic model on force feedback system for nano-newton force measurement"Proc. 16^<th> Int. Conf. on Force, Mass and Torque Measurements in Parallel with APMF '98. 338-345 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhiro Kato, Hisao Kikuta, Takashi Nakano, Toshiro Matsumoto and Koichi Iwata: "System analysis of the force-feedback method for force curve measurements"Rev. Sci. Instrum. 70 (5). 2402-2407 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshiro Matsumoto, Takashi Kannno, Nobuhiro Kato, Shigeki Hontsu, Kei Demizu, Toshiyuki Uenoya, and Tadataka Sugimura: "Formation of Biocompatible Hydroxyapatite Thin Films deposited by Laser Ablation"Proc. 12^<th> Int. Sympo. on Ceramics in Medicine. 499-502 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhiro Kato, Toshiro Matsumoto, Yasuhiro Nakamura, Hisashi Haccyou, Hisao Kikuta and Koichi Iwata: "Polymer micro cantilevers for force control atomic force microscopy"Proc. 5^<th> Asia-Pacific Sympo. on Measurement of Force, mass and Torque (APMF 2000). 91-96 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nobuhiro,Kato: "Polymer micro cantilevers for force control atomic force microscopy"Proceedings of the 5^<th> Asia-Pacific Symposium on Measurement of Force, mass and Torque (APMF2000). 91-96 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toshiro,Matsumoto: "Formation of Biocompatible Hydroxyapatite Thin Films deposited by Laser Ablation"Bioceramics. 12. 499-502 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nobuhiro,Kato: "System analysis of the force feedback method for force-curve measurements"Review of Scientific Instruments. 70(5). 2402-2407 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nobuhiro,Kato: "Dynamic model on force feedback system for nano-newton force measurement" Proceedings of IMEKO TC3/APMF‘98. 338-345 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuhiro,Kato: "System analysis of the force feedback method for force-curve measurements" Review of Scientific Instruments(1999/1/29 accepted).

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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