| Project/Area Number |
10650106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Materials/Mechanics of materials
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
YAMAMOTO Noritaka Ritsumeikan Univ. Fac. of Science and Engineering, Associate Professor, 理工学部, 助教授 (40210546)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Biomechanics / Collagen fiber / Tendon / Microstructure / Mechanical properties / Tensile test / Phase-contrast microscope / Differential interference microscope / コラーゲン / 線維 / 内側側副靭帯 |
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| Research Abstract |
We developed a new method by which tensile tests were performed observing the microstructure of the chemically non-treated raw collagen fascicles with a phase-contrast microscope or differential interference microscope, and studied the relationships between the microstructure and mechanical properties of fascicles obtained from the mouse tail tendon.
For mechanical testing, a specially designed micro tensile tester was mounted on a microscope. Small acrylic blocks were attached to both ends of each fascicle with cyanoacrylate adhesives. One of the blocks was fixed to a load cell, and the other one was fixed to the crosshead of a linear actuator. The fascicle was immersed in a saline solution and sandwiched between a slide glass and cover glass. The specimen was stretched to failure by moving the actuator at the speed of 0.1 mm/sec. The microstructure of the fascicle was observed using the microscope, and the images were recorded on a digital video recorder.
Microscopically, the bundles of collagen fibers arranged in a typical parallel fashion were observed in the fascicles. Under no load, the bundles had a periodic crimp pattern. At the liner region of load-deformation curve, the fibers were straightened in parallel. Before the catastrophic decrease of load, the partial rupture of some fibers was observed.
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