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Release characteristics of antibacterial substances included in cyclodextrin

Research Project

Project/Area Number 10650787
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionTottori University

Principal Investigator

FURUTA Takeshi  Tottori University, Biotechnology, Professor, 工学部, 教授 (10026164)

Co-Investigator(Kenkyū-buntansha) YOSHII Hidefumi  Tottori University, Biotechnology, Professor, 工学部, 助教授 (60174885)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordssurface protein / Getrichum sp., M111 cells / spray drying / cellulose-binding ability
Research Abstract

The cell with a functional surface protein such as cellulose-binding protein and glucoamylase is useful to the immobilized enzyme and food industry. Geotrichum sp. cell, M111 has an ability to accelerate the aggregation of cellulose fiber. We investigated the growth characteristics of M111 cells and the change of the aggregation ability of cellulose powder of vacuum and spray dried M111 cells. The following results were obtained.
1)The effects of temperature, pH, agitation speed, and the aeration velocity on the growth characteristics of M11 cells were investigated. The growth rate of M11 cells was markedly affected by the agitation speed, but was independent of the flow rate of air. The optimum temperature and pH for growth were 27 oC and 5, respectively.
2)The aggregation ability of cellulose powder with a yeast-like cell of M111 was characterized by the measurement of the aggregation-height in a glass micro-tube. The optimum pH and temperature of the aggregation were pH 4-5 and below 50 degree. The denaturation of M111 cell by heating could be well correlated with a first order denaturation kinetics. The activation energy of denaturation was 213kJ/mol. The aggregation was inhibited with surfactants such as SDS or STS.
3)M111 cells were dried at 80 oC under vacuum. The cellulose aggregation ability of the vacuum dried cells did not change and maintained the similar level to the original cells.
4)Spray drying method was applied to make a dried cell of M111. The inlet and the outlet temperature of air were 150 oC and 79oC, respectively. In this case, the cellulose aggregation ability of the spray-dried cells was decreased to 80% of the original ability. This implied that the decrease of the cellulose binding ability resulted from the shear stress at atomization.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] T. Furuta, H. Yoshii et al.: "Characterization of Cellulose-Binding Ability of Getrichum sp., M111 Cells and Application of Dehydration for Distilled Waste of Sweet Potato Shochu"Proceedings of Asian Pacific Conference of Chemical Engineering 99. 1097-1100 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Furuta, H.Yoshii et al.: "Characterization of Cellulose-binding Ability of Getrichum sp., M111 Cells and Application of Dehydration for Distilled Waste of Sweet Potato Shochu"Proceedings of Asian Pacific Conference of Chemical Engineering 99 Seoul, August 16-19. 1097-1100 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Furuta,H.Yoshii et al.: "Characterization of Cellulose-Binding Ability of Getrichum sp,M111 Cells and Application of Dehydration for Distilled Waste of Sweet Potato Shochu"Proceedings of Asian Pacific Conference of Chemical Engineering 99. 1097-1100 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Furuta, H.Yoshii et al.: "Characterization of Cellulose-binding Ability of Geotricum sp.Cell,M111" Proceedings of Asian Pacific conference of Chemical Engineering 99. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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