| Project/Area Number |
10650792
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業分析化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KANEKO Emiko Graduate School of Engineering, Tohoku University, Associate Researcher, 大学院・工学研究科, 助手 (00241539)
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| Co-Investigator(Kenkyū-buntansha) |
YOTSUYANAGI Takao Graduate School of Engineering, Tohoku University, Professor, 大学院・工学研究科, 教授 (00001199)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Whole Blood / Hemoglobin / Rhodamine B / ODS silica / Visual Fluorometry / 目視テスト / ドロップテスト |
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| Research Abstract |
It has been found that some dye solutes become concentrated along the perimeter of an aqueous drop on an octadecylsilanized (ODS) silica plate. There has been much theoretical and experimental interest recently in the formation of ring from dispersed micro-particles through evaporation. In this study, ODS plate serves as an solid surface to form a ring without evaporation. The fluorescent ring of Rhodamine B was applied to a visual test of hemoglobin in whole blood. When a drop of diluted blood and the dye is set on the plate, the resulting fluorescent ring can be observed through the drop with an ultraviolet lamp in the dark only for the samples of low hemoglobin content. The hydrophilic protein, hemoglobin, is not adsorbed on the plate. Its deep red color affects the excitation and visual perception of the fluorescent ring through the drop. The dye concentration was chosen to distinguish a slight difference between 120 and 100 g/L hemoglobin, which corresponds to healthy and slightly anemic female subjects, respectively.
In a separate experiment, it was found that the partition behavior of the triphenylmethane dyes on an ODS plate varied with the substituted groups on the three phenyl rings. This indicates that the ring formation would be atributed to the high hydrophobicity of the dye used.
The advantages of the proposed method can be applied to the screening test of anemia.
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