The Electron Transfer Mechanism of the Electrode Modified with the Enzyme Attached to Conductive Polymers
| Project/Area Number |
10650879
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子構造・物性(含繊維)
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
MIYAUCHI Shinnosuke Nagaoka University of Technology Faculty of Engineering, Professor, 工学部, 教授 (90018672)
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| Co-Investigator(Kenkyū-buntansha) |
YMAUCHI Takeshi Nagaoka University of Technology Faculty of Engineering, Assistant, 工学部, 助手 (90262477)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Biosensor / Glucose-sensing / Conductive Polymer / Carbon Fiber / Dopant / Polyisothianaphtene / ドーパンント / ポリ[1-(2-カルボキシエチル)ピロール] |
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| Research Abstract |
To investigate the electron transfer mechanism for the glucose sensing, the electrodes modified using conductive polymers, which were bound with the glucose oxidase (GOD) were prepared. Then, a large stabilized current was obtained. In the meanwhile, the sensor of which the conductivity was big without the dopant was produced in order to remove the effect of the dopant. By copolymerizing isothianaphtene and [1-(2-carboxypyrrole)] in 9:1, the sensor without dopant was produced. In this sensor, the reproducibility was excellent. It was also regarded that the electrode surface area was increased. Carbon fiber woven fabric was used as an electrode. Through the conjugation of π electron, the conduction occurs two-dimensionally in this system. Here, the functional group was introduced by dealing with the carbon surface in oxygen plasma, and many enzymes were fixed on the surface. It was possible to introduce GOD which exceeds 60 μg/cmィイD12ィエD1 as the result, and it was possible that sensor with high-density GOD is produced. It was possible to obtain big detection current over 60 μA for glucose used. It was recognized that the detection current depended on the quantity of GOD of the film surface.
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Report
(3 results)
Research Products
(7 results)
