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Fine structural analysis of rice wx and the application for breeding

Research Project

Project/Area Number 10660001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

INUKAI Tsuyoshi  Hokkaido Univ., Grad. School of Agr., Inst., 大学院・農学研究科, 助手 (90223239)

Co-Investigator(Kenkyū-buntansha) SANO Yoshio  Hokkaido Univ., Grad. School of Agr., Pro., 大学院・農学研究科, 教授 (70109528)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsOryza sativa / wx gene / Genetic recombination / Oryza sativa / 遺伝的組換え
Research Abstract

In plant genomes as well as other eukaryotic genomes meiotic recombination does not occur uniformly. At the level of the gene, high recombination frequencies are often observed within genetic loci in maize, but this feature of intragenic recombination is not seen at the csr1 locus in Arabidopsis. These observations suggest that meiotic recombination in plant genomes varies considerably among species. In the present study we investigated meiotie recombination at the wx locus in rice. The mutation sites of wx mutants induced by ethyl methanesulfonate (EMS) treatment or gamma-ray irradiation and a spontaneous wx mutant were physically characterized, and the genetic distances between those wx mutation sites were estimated by pollen analysis. Based on these results, the recombination frequency at the wx locus in rice was estimated as 27.3 kb/cM, which was about ten times higher than the average for the genome, suggesting that there was a radically different rate of meiotic recombination for intra- and intergenie regions in the rice genome.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Tsuyoshi Inukai: "Analysis of intragenic recombination at wx in rice : correlation between the molecular and genetic maps with in the locus"Genome. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuyoshi Inukai: "Frequency and distribution of intragenic reconbination at the wx locus in rice"Rice Genetics Newsletter. 15. 169-170 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Khin-Thidar: "Distribution of recombination sites in the 195-kb region surrounding the wx gene in rice"Rice Genetics Newsletter. 16. 59-61 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D.-H.Chen: "Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar"Theoretical and Applied Genetics. 98. 1046-1053 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] L.V.Dung: "Dissection of a major QTL for photoperiod sensitivity in rice : its association with a gene expressed in an age-dependent manner"Theoretical and Applied Genetics. 97. 714-720 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Inukai, A. Sako, H.-Y. Hirano and Y. Sano: "Analysis of intragenic recombination at wx in rice : correlation between the molecular and genetic maps within the locus"Genome. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Inukai, A. Sako, Khin-Thidar and Y. Sano: "Frequency and distribution of intragenic recombination at the wx locus in rice"Rice Genetics Newsletter. 15. 169-170 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Khin-Thidar, Y. Tanaka, T. Inukai and Y. Sano: "Distribution of recombination sites in the 195-kb region surrounding the wx gene in rice"Rice Genetics Newsletter. 16. 59-61 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D.-H. Chen, M. dela Vina, T. Inukai, D. J. Mackill, P. C. Ronald and R. J. Nelson: "Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar"Theoretical and Applied Genetics. 98. 1046-1053 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] L. V. Dung, T. Inukai and Y. Sano: "Dissection of a major QTL for photoperiod sensitivity in rice : its association with a gene expressed in an age-dependent manner"Theoretical and Applied Genetics. 97. 714-720 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuyoshi Inukai: "Analysis of intragenic recombination at wx in rice : correlation between the molecular and genetic maps within the locus"Genome. in press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuyoshi Inukai: "Frequency and distribution of intragenic recombination at the wx locus in rice"Rice Genetics Newsletter. 15. 169-170 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Khin-Thidar: "Distribution of recombination sites in the 195-kb region surrounding the wx gene in rice"Rice Genetics Newsletter. in press. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] D.-H.Chen: "Molecular mapping of the blast resistance gene, Pi44(t), in a line derived from a durably resistant rice cultivar"Theoretical and Applied Genetics. 98. 1046-1053 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] L.V.Dung: "Dissection of a major QTL for photoperiod sensitivity in rice : its association with a gene expressed in an age-dependent manner"Theoretical and Applied Genetics. 97. 714-720 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 犬飼 剛: "イネにおける遺伝子座内組換えの様相-wx遺伝子座の場合-" 育種学雑誌. 48(別1). 15 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 佐古 彩: "イネwx遺伝子座におけるγ線及びEMS誘発変異の特徴" 育種学雑誌. 48(別2). 330 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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