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Construction of gene rearrangement system by RMu transposon in rice

Research Project

Project/Area Number 10660003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHIROSAKI UNIVERCITY

Principal Investigator

ISHIKAWA Ryuji  Hirosaki Univ.Faculty of Agriculture and Life Scie.associate Professor, 農学生命科学部, 助教授 (90202978)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsOryza sativa / rice / transposon / 非相同組換え / イネ / RMu / Transposon / RiceMutator / I-PCR / mutations
Research Abstract

An Indica cultivar of rice carries a 4374 bp element flanked by a 9 bp genomic repeat. The ends of this element comprise 193 bp terminal inverted repeats (TIRs) that are identical in their most terminal 90 bps and 87% identical throughout. Between the TIRs, there is an open reading frame that translates to a protein that is homologous to the transposase MURA encoded in the autonomous maize Mu element MuDR.On the basis of overall structure, the characteristic 9 bp genomic repeat and MURA homology, we conclude that the rice element, RMul is a close and obvious relative of the autonomous maize transposon MuDR, and is more related to maize elements than to any Mu-type element previously reported in rice. Copy number of RMu elements is moderate in all cultivars examined. Transcript of the orf of RMul-IR36 was detected in some Japonica strains. Two kinds of splicing products were transcribed, and both specify in-frame product. Indica lines did not transcribe RMu. However, cold stress given in intervals activated transcription of the rmuA gene in RMu-IR36, and the primary transcript was correctly processed. The specificity of induction conditions was surprising. Database searches found that RMu in rice is a member of the same branch of Angiosperm Mu elements as is the autonomous element MuDR in maize, except RMul does not contain a mudrB ortholog. This is the first such element to be found.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Miyashita et al: "Copy number of RMu elemenets in Japanese lowland rice landrace"Rice Genet.Newslt.. 15. 162-162 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishikawa,R. et al.: "Behavior of transposable elements in Akihikari and its parental strains"Rice.Genet.Newlt.. 15. 163-164 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyashita et al.: "Copy number of Rmu elements in Japanese lowland rice landrace"Rice Genet.Newslt.. 15. 162 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishikawa et al.: "Behavior of transposable elements in Akihikari and Its parental strains."Rice Genet.Newslt.. 15. 163-164 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyashita et al.: "Copy number of RMu elemenets in Japanese lowland rice landrace."Rice Genet.Newslt.. 15. 162-162 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishikawa,R. et al.: "Behavior of transposable elements in Akihikari and its parental strains."Rice.Genet.Newlt.. 15. 163-164 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Miyashita et al: "Copy number of RMu elements in Japanese lowland race" Rice Genetic Newsletter. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] R.Ishikawa et al: "Behavior of Transposable elements in Akihikari and its Darental Strains" Rie Genetics Newsletter. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Sunako et al.: "An allile of the ripening-specific 1-aminocyclopropane-1-carboxylic acid synthase gene(ACSI)in apple fvit with a long storage life." Plant Phsyiol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Shimizu et al.: "Enhanced expression and differential in ducibility of soybean chalcone synthase genes by supplemental UV-B in dark-grown seedlings" Plant Mol.Biol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Harada et al.: "Md-ACSl,atruit ripening 1-aminocyclopropane-1-carboxylate synthase gene of apple" Breeding and Biotechnology for Fruit Trees NIFTS/CSTRO. 42-45 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Kaimori et al: "Asymmetric somatic cell hybrids between alfalfa and birds foot trefoil" Breeding Science. 48. 29-34 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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