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Utility of minor somaclonal variation and detection of the genetic mutation in rice.

Research Project

Project/Area Number 10660005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

ABE Toshinori  Yamagata University, 農学部, 助教授 (80202670)

Co-Investigator(Kenkyū-buntansha) SASAHARA Takeo  Yamagata University, 農学部, 教授 (20005606)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsOryza sativa L. / Somaclonal variation / short culm / Chloroplast DNA / Southern hybridization / DNA Delation / Oryza sastiva L. / AFLP / 培養変異 / PCR / フィンガープリント / 欠失
Research Abstract

1. Somaclomnal variation obtained from short term culture of rice calli. Somaclonal variation obtained from tissue culture regenerants in expected for crop improvement. We obtained 10 regenerated plants from seed calli of japonica variety, Reimai. The plants were grown in green house until mature stage and analyzed agronomic traits. Out of 10 regenerants, 3 plants were 8-10 cm shorter that Reimei and other regenerated plants. While, one regenerated plant showed fewer ripened grains and lower ripening percentage. The other characters were almost the same. From these results, It should be clear the genes controlling the culm length in these regenerated plants.
2. Detection of genomic change at molecular level coused by long term culture of rice calli. Chloroplast DNA (ctDNA) alteration of long term callus cultures derived from rice seeds was easily detected when rice chloroplast DNA fragment BamHI-1 was used as a probe. Chloroplast DNA (ctDNA) molecules of two callus lines out of 12 callus lines cultured for 36 months were deficient in large regions of the rice chloroplast genome. Maps of deleted ctDNA molecules were constructed using a seventeen hybridization probes. In the callus lines of T36-1 and T36-2, small sizes of ctDNA molecules (58.5 and 7.5 kb) were mapped. When these two callus lines were further cultured on the MS maintenance medium for more six months, further deletion (about 0.5 kb) was occurred in one callus line (T42-2). From the restriction maps of the deleted derivatives of ctDNA, callus lines of T36-1 and T42-1 lost both large inverted repeat sequences, together with the intervening small single copy region. On the other hand, callus lines of T36-2 and T42-2 lost rbcL and most of the large single copy region. These ctDNA molecules commonly retain only about 20 kb that encode tm gene cluster and rpo genes. These results suggests that this region of rice chloroplast genome contains at least one functional origin of replication.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report

Research Products

(4 results)

All Other

All Publications (4 results)

  • [Publications] 阿部利徳・長沢和永・笹原健夫: "イネソマクローナル変異のAFLP法による解析"育種学雑誌. 48別2. 3 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] "AFLP analysis of somaclonal mutation in rice."Breeding Science. 48, Suppl 2. 3 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 阿部利徳、長沢和永、笹原健夫: "イネソマクローナル変異のAFLP法による解析"育種学雑誌. 48別2. 3 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 阿部利徳 ら: "イネソマクローナル変異のAFLP法による解析" 育種学雑誌. 48(別2). 3 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1999-03-31   Modified: 2016-04-21  

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