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Identification of Juvenile hormone-dependent transcription factors by using yeast system

Research Project

Project/Area Number 10660045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionMie University

Principal Investigator

MIURA Ken  Mie University, Faculty of Medicine, Lecturer, 医学部, 講師 (60219582)

Co-Investigator(Kenkyū-buntansha) CHINZEI Yasuo  Mie University, Faculty of Medicine, Professor, 医学部, 教授 (60024709)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsJuvenile hormone / ecdysone / nuclear receptor / mosquito / transcription factor / coactivator / コアクティベーター
Research Abstract

As an initial step toward the identification of Juvenile hormone(JH)-dependent transcription factors, cDNA cloning, sequence analysis and functional dissection of potential coactivators and basel transcription factors involved in ecdysone (20E) signaling in mosquitoes were done, giving the following results :
1. cDNA cloning mosquito homologs of Bx42, GCN5, TBP, CBP and MBF1 were taken place. As for Bx42, GCN5 and TBP, full length cDNA clones were isolated, and their sequence and phylogenetic analyses were performed.
2. By introducing a luciferase reporter construct driven by 20E response elements into mammalian and Drosophia cell lines, transient transfection systems, where the transcription from the reporter construct was enhanced greatly upon the addition of the ligand or its analog in culture media, were established.
3. By utilizing the transient cell transfection systems mentioned above, both Bx42 and GCN5 were proved to function as coactivators in 20E-dependent gene activation.
4. Mammalian two-hybrid assay revealed the protein-protein interaction of Bx42 of GCN5 against ecdysone receptor protein.
5. Nuclear localization of Bx42 was confirmed by cell transfection experiments with a construct which expresses Bx42-EGFP fusion protein.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Wang, S-F et al.: "DNA binding and transactivation characteristics of the mosquito ecdysone receptor-ultraspiracle complex"J. Biol. Chem.. 273. 27531-27540 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miura, K et al.: "Two hexameric cyanoprotein subunits from an insect, Riptortus clavatus : sequence, phylogeny and developmental amd juvenile horpche regulation"Eur. J. Biochem.. 258. 929-940 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Raikhel, AS et al.: "Nuclear receptors in mosquito vitellogenesis"Am. Zool.. 39. 722-735 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miura, K et al.: "Two distinct subpopulations of ecaysone receptor complex in the female mosquito during vitellogenesis"Mol. Cell. Endocrinol.. 156. 111-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhu, J et al.: "AHR38, a homolog of NGFI-B, inhibits formation of the functional ecdysteroid receptor in the mosquito aedes algypti"EMBO J.. 19. 253-262 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang, S-F et al.: "Differential expression and regulation by 20-hydroxyecdysone of mosquito ultraspiracle isoforms"Dev. Biol.. 218. 99-113 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang, S-F et al.: "DNA binding and transactivation characteristics of the mosquito ecdysone receptor-ultraspiracle complex"J. Biol. Chem.. 273. 27531-27540 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miura K. et al.: "Two hexameric cyanoprotein subunits from an insect, Riptortus clavatus : Sequence, Phylogeny and development and juvenile hormone regulation"Eur. J. Biochem.. 258. 929-940 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Raikhel, AS et al.: "Nuclear receptors in mosquito vitellogenesis"Am. zool.. 39. 722-735 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Miura K. et al.: "Two distinct subpopulations of ecdysone receptor complex in the female mosquito during vitellogenesis"Mol. cell. Endocrinol. 156. 111-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhu, J-S et al.: "AHR38, a homolog of NGFI-B, inhibits formation of the functional ecdysteroid receptor in the mosquito Aedes aegypti"EMBO J.. 19. 253-262 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang, S-F et al.: "Differential expression and regulation by 20-hydroxyecdysone of mosquito ultraspiracle isoforms"Dev. Biol.. 218. 99-113 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wang,S-F et al.: "DNA binding and transactivation characteristics of the mosquito ecdysone receptor-ultraspiracle complex"J.Biol.Chem. 273・42. 27531-27540 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miura,K et al.: "Two hexameric cyanoprotein subunits from an insect,Riptortus clavatus : sequence,phylogeny and developmental and juvenile hormone regulation"Eur.J.Biochem.. 258・3. 929-940 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Raikhel,AS et al.: "Nuclear receptors in mosquito vitellogenesis"Am.Zool.. 39. 722-735 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miura,K et al.: "Two distinct subpopulations of ecdysone receptor complex in the female mosquito during uitellogenesis"Mol.Cell.Endoccrinol.. 156. 111-120 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhu,J et al.: "AHR38,a homolog of NGP1-B,inhibits formation of the functional ecdysteroid receptor in the mosquito Aedes aegypti"EMBO.J.. 19・2. 253-262 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wang,S-F et al.: "Differential expression and regulation by 20-hydroxyecdysone of mosquito ultraspiracle isoforms"Dev.Biol.. 218. 99-113 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miura,K.et al.: "Two hexameric cyanoprotein subunits from an in sect,Riptertus clavatus: sequence, phylogeny and developmental and Juvenile hcrniene regulation." Eur.J.Biochem. 258・3. 929-940 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sun,J.et al: "Characterization and cDNA cloning of a hemoprotein in the salivary glands of the blood-sucking insect,Rhodonius prolixus" Insect Biochem.Mol.Biol.28・3. 191-200 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wang,S.-F.et al: "DNA binding and fransactiration Characteristics of teh mosyuito ecdysone receptor-ultraspiracle complex" J.Biol.Chem. 273・42. 27531-27540 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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