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Studies on molecular interactions in two closely related plant viruses putatively using different mechanisms for cell-to-cell movement

Research Project

Project/Area Number 10660048
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MISE Kazuyuki  Kyoto University, Grad School of Agriculture, Associate Professor, 農学研究科, 助教授 (90209776)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBromoviruses / Cell-to-cell movement / Coat protein / Movement protein / Chimeric virus / Green fluorescent protein / Tubular structure / Glutathion S-transferase / プロモウイルス
Research Abstract

Brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) are closely related plant viruses but suggested to use different mechanisms for cell-to-cell movement in view of its coat protein (CP) requirement. CP-defective variants of CCMV move from cell to cell but those of BMV do not. To determine the viralfactor (s) involved in the CP requirement in viral cell-to-cell movement, we made several chimeric viruses, e.g. BMV (ΔCP-GFP) and CCMV (ΔCP GFP), where green fluorescent protein (GFP) gene was substituted for the CP. The 3a movement protein (MP) genes were exchanged between the chimeras to construct BMV (C3a/ΔCP-GFP) and CCMV (B3a/ΔCP-GFP). Although BMV (ΔCP-GFP) did not move from cell to cell and CCMV (ΔCP-GFP) moved, BMV (C3a/ΔCP-GFP) moved and CCMV (B3a/ΔCP-GFP) did not move, demonstrating that bromovirus 3a genes determine the requirement of the CP gene for viral spread. We have showed that BMV 3a binds single-stranded (ss) RNA in vitro. Here, I demonstrated that the CCMV 3a also binds ss-RNA cooperatively without sequence specificity. To examine tubule-forming capacity of the 3a proteins of BMV and CCMV, these proteins were monitored in protoplasts as fusion proteins with GFP. In protoplasts, tubular structures were induced from their surface, when they were inoculated with BMV or CCMV derivatives encoding 3a-GFP fusions. Together, one possible explanation is that BMV and CCMV could take at least two mechanisms for movement. Either virus may move through tubular structures as well as could traffic plasmodesmata as MP-RNA complex. GST pull-down assay showed that BMV MP binds its CP in vitro. Further studies are required to understand correlation between CP requirement of each virus and binding ability of each MP with CP.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Kobayashi,K.: "Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts"Microbiol.Immunol.. 42. 65-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita, M.: "Nucleic acid-binding properties and subcellular localization of the 3a protein of brome mosaic bromovirus"J.Gen.Virol.. 79. 1273-1280 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi,K.: "Evidence for a dual strategy in the expression of cauliflower mosaic virus open reading frames I and IV"Microbiol.Immunol.. 42. 329-334 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi,K.: "Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts"Microbiol.Immunol.. 42. 377-386 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita, Y.: "Genotypic and phenotypic analysis of bromovirus adaptive mutants derived from a single plant"Microbiol.Immunol.. 43. 181-185 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Damayanti,T.A.: "Brome mosaic virus defective RNAs generated during infection of barley plants"J.Gen.Virol.. 80. 2511-2518 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuge,S.: "Cauliflower mosaic virus ORF III product forms a tetramer in planta : its implication in viral DNA folding during encapsidation"Microbiol.Immunol.. 43. 773-780 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita,M.: "Expression and characterization of the 3a movement protein of cowpea chlorotic mottle bromovirus"Arch.Virol.. 144. 2449-2456 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagano,H.: "The cognate coat protein is required for cell-to-cell movement of a chimeric brome mosaic virus mediated by the cucumber mosaic virus movement protein"Virology. 265. 226-234 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 三瀬和之: "ブロモウイルスの細胞間移行と植物への適応機構."ウイルス. 49. 95-106 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inagaki,A.: "Construction of an equalized cDNA library from Colletotrichum lagenarium and its application to the isolation of differentially expressed genes"Can.J.Microbiol.. 46. 150-158 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takano,Y.: "The Colletotrichum lagenarium MAP kinase gene CMK1 regulates diverse aspects of fungal pathogenesis"Mol.Plant-Microbe Interact.. 13. 374-383 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi, K.: "Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts."Microbiol.Immunol.. 42(1). 65-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita, M.: "Nucleic acid-binding properties and subcellular localization of the 3a protein of brome mosaic bromovirus."J. Gen. Virol.. 79. 1273-1280 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi, K.: "Evidence for a dual strategy in the expression of cauliflower mosaic virus open reading frames I and IV."Microbiol.Immunol.. 42(4). 329-334 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kobayashi, K.: "Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turmip protoplasts."Microbiol.Immunol.. 42(5). 377-386 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita, Y.: "Genotypic and phenotypic analysis of bromovirus adaptive mutants derived from a sinle plant."Microbiol. Immunol.. 43(2). 181-185 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Damayanti, T. A.: "Brome mosaic virus defective RNAs generated during infection of barley plants."J. Gen. Virol.. 80. 2511-2518 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsuge, S.: "Cauliflower mosaic virus ORF III product forms a tetramer in planta : its implication in viral DNA folding during encapsidation."Microbiol. Immunol.. 43(8). 773-780 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita, M.: "Expression and characterization of the 3a movement protein of cowpea chlorotic mottle bromovirus."Arch. Virol.. 144(12). 2449-2456 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagano, H.: "The cognate coat protein is required for cell-to-cell movement of a chimeric brome mosaic virus mediated by the cucumber mosaic virus movement protein."Virology. 265(2). 226-234 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mise, K.: "Mechanisms of bromovirus cell-to-cell movement and adaptation to plants."Virus.. 49(2). 95-106 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inagaki, A.: "Construction of an equalized cDNA library from Colletotrichum lagenarium and its application to the isolation of differentially expressed genes."Can. J. Microbiol.. 46(2). 150-158 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takano, Y.: "The Colletotrichum lagenarium MAP kinase gene CMK1 regulates diverse aspects of fungal pathogenesis."Mol.Plant-Microbe Interact.. 13(4). 374-383 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujita,Y.: "Genotypic and Phenotypic analysis of bromovirus adaptive mutants derived from a single plant"Microbiol. Immunol.. 43(2). 181-185 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Damayanti,T.A.: "Brome mosaic virus degective RNAs generated during infection of barley plants"J.Gen.Virol.. 80. 2511-2518 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsuge,S.: "Cauliflower mosaic virus ORF III product forms a tetramer in planta : its implication in viral DNA folding during encapsidation"Microbiol. Immunol.. 43(8). 773-780 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujita.M.: "Expression and characterization of the 3a movement protein of cowpea chlorotic mottle bromovirus"Arch.Virol.. 144(12). 2449-2456 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagano,H.: "The cognate coat protein required cell-to-cell movement of a chimeric brome mosaic virus mediated by the cucumber mosaic virus movement protein"Virology. 265(2). 226-234 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三瀬和之: "プロモウイルスの細胞間移行と植物への適応機構"ウイルス. 49(2). 95-106 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Inagaki,A.: "Construction of an equalized cDNA library from colletotrichum lagenarium and its application to the isolation of differentially expressed genes"Can.J.Microbiol.. 46(2). 150-158 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takano,Y.: "The Colletotrichum lagenarium MAP kinase CMKI regulates diverse aspects of fungal pathogenesis"Mol.Plant-Microbe Interact.. 13(4). 374-383 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kobayashi,K.: "Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts." Microbiol.Immunol.42(1). 65-69 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujita,M.: "Nucleic acid-binding properties and subcellular localization of the 3a protein of brome mosaic bromovirus." J.Gen.Virol.79. 1273-1280 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi,K.: "Evidence for a dual strategy in the expression of cauliflower mosaic virus open reading frames I and IV." Microbiol.Immunol.42(4). 329-334 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi,K.: "Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts." Microbiol.Immunol.42(5). 377-386 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujita,Y.: "Genotypic and phenotypic analysis of bromovirus adaptive mutants derived from a single plant." Microbiol.Immunol.43(2). 181-185 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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