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Studies on the cloning of few-coiled type of spore in entomopathogenic microsporidia

Research Project

Project/Area Number 10660056
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionNihon University

Principal Investigator

IWANO Hidetoshi  Nihon Univ. Bioresource Sci. Assoc. Prof., 生物資源科学部, 助教授 (40120381)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsmicrosporidia / spore dimorphism / cloning / Nosema bombycis / micromanipulator / Vairimorpha necatrix / isolation / cell culture / Nosema / Vairimorpha
Research Abstract

The purpose of this study was to clone entomogenous microsporidia by infecting an insect cultured cell with a single spore. In an attempt to isolate the octospore of Vairimorha necatrix that had been propagated in silkworm larvea reared at 16゜C. In the genus Nusema, one of the two types of the spore, the few-coiled type was recovered from cultured Helicoverpa zea cells infected with Nosema furnacalis and cultured Antheraea eucalypti cells infected with N. bombycis.
A single spore was recovered from each suspension of few-coiled type spores and transferred to a cell culture using a micromanupulator.
Although the developmental stages of N. furnacalis were detected in cultured H. zea cells after 30-day incubation, those of N. bombycis were not. When a single spore of many-coiled type of N. bombycis spore was transferred to cell cultures after alkaline activation, various developmental stages were detected in cultured A. eucalypti cells after 30-day incubation. The cloned microsporidia from a single spore could be subcultured by supplementing the culture with the healthy cells.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Elizabeth U. Canning: "Nosema tyriae n.sp. and Nosema sp., microsporidian parasites of cinnabar moth Tyria jacobaeae"Journal of Invertebrate Pathology. 74. 29-38 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] E. U. Canning, A. Curry, S. A. Cheney, N. J. Lafranchi-Tristem, Y. Kawakami, Y. Hatakeyama, H. Iwano and R. Ishihara: "Nosema tyriae n.sp. And Nosema sp., microporidian parasites of cinnabar moth Tyria jacobaeae"J. Invertebrate Pathology. 74. 29-38 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Elizabeth U. Canning: "Nosema tyriae n.sp. and Nosema sp., microsporidian parasites of cinnabar moth Tyria jacobaeae"Journal of Invertebrate Pathology. 74. 29-38 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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