Research and classification of Zn-Bacteriochlorophyll-containing bacteria

• Project/Area Number: 10660069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 応用微生物学・応用生物化学
• Research Institution: IWATE UNIVERSITY
• Principal Investigator: WAKAO Norio Iwate University, Department of Bioscience and Technology, Professor, 農学部, 教授 (30003784)
• Project Period (FY): 1998 – 1999
• Project Status: Completed (Fiscal Year 1999)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: photosynthetic pigments / Zn-bacteriochlorophy11 / acidophilic bacteria / Acidiphilium

Research Abstract

It has been thought for a long time that Mg-(bacterio)chlorophylls are essential for the photosynthesis in nature. However, Zn-containing bacteriochlorophyll (Zn-BChl) was discovered to be present in the acidophilic aerobic heterotrophic bacteria, genus Acidiphilium, and to be functional in cells. In this study we investigated widely the bacteria with Mg-BChl and/or Zn-BChl in natural acid environments.
1. We extracted and analysed the pigments by organic solvents from the acid natural environments such as waters, soils, and biomat. But the pigments could not be detected.
2. We tried to detect the puf genes of photosynthetic systems with Mg-BChl and/or Zn-BChl by PCR method but obtained no positive results.
3. We collected many acidophilic aerobic heterotrophic bacteria from many acid environments such as acid mine waters, acid hot springs, acid biomat, and analysed the pigments in living cells of the isolates. As the results, relatively many isolates with the Mg-BChl were found but Zn-BChl producers were rarely.