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ANALYSIS OF A NEW FACTOR RELATED WITH THE SIGNAL TRANSDUCTION IN FISSION YEAST

Research Project

Project/Area Number 10660087
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionSHIMANE UNIVERSITY

Principal Investigator

KAWAMUKAI Makoto  SHIMANE UNIVERSITY, LIFE AND ENVIRONMENTAL SCIENCE, PROFESSOR, 生物資源科学部, 教授 (70186138)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Tsuyoshi  SHIMANE UNIVERSITY, MOLECULAR INSTITUTE OF GENETICS, ASSOCIATE PROFESSOR, 遺伝子実験施設, 助教授 (30202211)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsRAS / cAMP / Signal transduction / fission yeast / 14-3-3 / Byr2 / slal / 14・3・3 / cAMP
Research Abstract

Schizosaccharomyces pombe expresses two homologs of mammalian 14-3-3 genes : rad24, which is involved in checkpoint control, and rad25, the function of which is considered to be supplementary to rad24 activity. In this study, we present evidence that both Rad24 and Rad25 act as negative regulators of Byr2 (MAPKKK). Overexpression of either rad24 or rad25 reduced sporulation efficiency in homothallic wild-type cells. In contrast, the sporulation efficiency of rad24 and rad25 null cell was higher than that of wild-type cells. Deletion of rad24 or rad25 increased sporulation efficiency in ras1 null cells, but not in byr2 or ste4 null cells. Rad24 and Rad25 had no effect on the activity of constitutively active Byr1S214DT218D. Rad24 and Rad25 bound to both the N-terminal and the C-terminal domains of Byr2 in the yeast two-hybrid system. The formation of complexes in vivo was also confirmed by immuno-coprecipitation. Furthermore, we showed that an antibody against phospho-p44/42 MAP kinase recognized elevated phosphorylation levels of Spk1 protein (MAPK) in rad24 and rad25 null cells, and that the mRNA level of mam2, which is regulated by Ras1, was clearly elevated in ras1 rad24 and ras1 rad25 null cells relative to ras1 null cells. The cellular localization of Rad24, Rad25 and Byr2 was examined using fusion to green fluorescent protein (GFP). While Byr2-GFP translocated from the cytoplasm to the plasma membrane in response to nutrient starvation, Rad24-GFP and Rad25-GFP remained in the cytoplasm. Our of all results consistently indicate that Rad24 and Rad25 bind to Byr2 in the cytoplasm and negatively regulate Byr2 signaling in the sexual development in S.pombe.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Gou-Lei Zhonら: "Identification of a CAP homologous gene in lentinus edodes and its functional complementation of yeast CAP mutant"Microbiology. 144. 1085-1093 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okadaら: "Biological significans of the side chain length of obiquinone in saccharomyces cerrisiae"FEBS Lett.. 255. 52-59 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawamukai: "Isolation of a novel gene, mx2 encoding a putative DNA helicase as a suppressor of sterile strain in S pombe"Biochem. Biophs. Acta. 1446. 93-101 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tanakaら: "Characterization of fission yeast SUM of homologene P3t3p, required multiple nuclear events including the control telomea"Mol. Cell. Biol.. 19. 3660-3677 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Gou-Lei Zhonら: "Identification of a 14-3-3 protein for lentinus edodes that interacts with CAP (adsyly cyclase-associated protein)"Biosci. Biotech. Biochem.. 64. 149-159 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 川向 誠: "分裂酵母の分化を制御する情報伝達系の解析"農芸化学会誌. 73. 1147-1153 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 川向 誠: "ポイントがわかる分子生物学"丸善(4頁). 128 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Guo-Lei Zhou, Katsunori Tanaka, Tsuyoshi Nakagawa, Hideyuki Matsuda and Makoto Kawamukai: "Identification of a CAP (adenylyl cyclase-associated protein) homologous gene from Lentinus edodes and its functional conservation with yeast CAPs"Microbiology. 144. 1085-1093 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Makoto Kawamukai: "Signal transduction in fission yeast. in 'The structure and function of gene.'"Gakkai Shuppannsha senta (Japanese). 87-96 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kazunori Okada, Tomohiro Kainou, Hideyuki Matsuda and Makoto Kawamukai: "Biological significance of the side chain length of ubiquinone in Saccharomyces cerevisiae"FEBS lett.. 431. 241-244 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Makoto Kawamukai: "Isolation of a novel gene, moc2, encoding a putative RNA helicase as a suppressor of sterile strains in Schizosaccharomyces pombe"Biochim. Biophys. Acta.. 1446. 93-101 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Makoto Kawamukai: "Analysis of signal transduction that regulates the differentiation of fission yeast"Nougeikagakukaishi (Japanese). 73. 1147-1153 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katsunori Tanaka, Koei Okazaki, Junko Nishide, Osamu Niwa, Hideyuki Matsuda, Makoto Kawamukai and Yota Murakami.: "Characterization of a fission yeast SUMO-1 homologue, Pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation"Mol. Cell. Biol.. 19. 8660-8672 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Guo-Lei Zhou, Takaharu Yamamoto, Fumiyo Ozoe, Daisuke Yano, Katsunori Tanaka, Hideyuki Matsuda and Makoto Kawamukai: "Identification of a 14-3-3 protein from Lentinus edodes that interacts with CAP (adenylyl cyclase-associated protein), and conservation of this interaction in fission yeast"Biosci. Biotech. Biochem.. 64. 149-159 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawamukaiら: "Isola of a novelgene moc2 encoding a pufative RNA helicase as a suppussor of strain in S pombe"Biochim Biophys. Acta. 1446. 93-101 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 川向誠: "分裂酵母の分化を制御する情報伝達系の解析"農芸化学会誌. 73. 1147-1153 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanakaら: "Charictenzation of a fission yeast SUMO-1 homologue, Pmt 3p, regained for multiple nuleel events including the controle1 tdomre"Mol Cell Biol. 19. 3660-3671 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 川向 誠: "ポイントがわかる分子生物学"丸善. 4頁/12頁 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Gou-Lei Zhou ら: "Identification of o CAP homologons gene in Lentinas eclcdes and its functional complementation of yeast CAP mutants" Microbiology. 144. 1085-1093 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okada. Kazunori ら: "Biological significanse of the side chain length of ubiquinone in Sacchoromyces cerevisine." FEBS Lett.255. 52-59 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 川向 誠: "分裂酵母のシグナル伝達系" 遺伝子の構造と機能(学会出版センター). 87-96 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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