Studies on high-dimensional functions of glycoconjugates toward development of new medicines and biomaterials

• Project/Area Number: 10660107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: GIFU UNIVERSITY
• Principal Investigator: ISHIDA Hideharu Gifu University, Associated Professor, 農学部, 助教授 (20203002)
• Project Period (FY): 1998 – 1999
• Project Status: Completed (Fiscal Year 1999)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: Autoimmune Disease / Neural injury / Auto antibody / Ganglioside / Guillain-Barre / 糖鎖工学 / 微量ガングリオシド / ギラン・バレー症候群 / カンピロバクター・ジェジュニ / リポ多糖

Research Abstract

Certain species of anti-gangliosides are associated with specific clinical features in various neurologic diseases such as Guillain-Barre syndrome (GBS). In this project, two kinds of glyco-conjugates were synthesized to clarify the mechanism to cause GBS as well as the mechanism to produce autoantibodies to gangliosides. One is the minor ganglioside with the complex structure, GalNAc-GD1a, Lactoganglio-1 and Lactoganglio-2, which are found in nervous system. Another is Lipopolysaccharide (LPS) of Campylobacter jejuni (C.jejuni) which is known to infect the patients of GBS prior to appearance of GBS.All of the target molecules have been synthesized successfully in our hands, and the evaluation of the functions of these molecules are now under investigation.

Research Products

• [Publications] Sawada et al.: "The first synthesis of glycan parts of lactoganglio-and neolacto"Tetrahedron Letters. 42. 1745-1747 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sawada, N., Ito, M., Ishida, H.and Kiso, M.: "The first synthesis of glycan parts of lactoganglio- and neolactoganglio-series gangliosides"Tetrahedron Lett.. 42. 1745-1747 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] H.Ando et al.: "Synthetic Studies on Sialoglycoconjugates 110: Efficient Assembly of α-linked tetrameric sialoglycosides coupled with galactose and lactose"J.Carbohydr.Chem.. 18(5). 603-607 (1999)
• [Publications] M.Okada et al.: "Antibodies to GDI2 and to GQIB in Guillain-Barre Symdrome and the related disoeder"Neurological Sciences. 165. 126-132 (1999)
• [Publications] N.Sawada et al.: "Ganglioside GDI2 analogues as high-affinity ligands for myelin-associated glycoprotein(MAG)"Carbohydr.Res,. 316. 1-5 (1999)
• [Publications] X.Zhang et al.: "Chemoenzymatic Synthesis of ganglioside GM4 analogs as potemeial immunosuppressive agents"J.Carbohydr.Chem.. 18(2). 225-239 (1999)
• [Publications] S.Komba et al.: "The first total synthesis of 6-sulfo-de-N-acetylsialyl Lewis X ganglioside: a superior ligand for human L-selectin"Angew.Chem.Int.Ed. 38(8). 1131-1133 (1999)
• [Publications] S.Komba: "First total synthesis of 6-sulfo De-N-acetyl-sialyl Lewis X Ganglioside : a superior ligand for human L-Selectin." Angew.Chem.(1999)