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Lectin-induced enhancement of superoxide anion production by red tide plankton, Chattonella marina

Research Project

Project/Area Number 10660186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNagasaki University

Principal Investigator

ODA Tatsuya  Nagasaki University, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (60145307)

Co-Investigator(Kenkyū-buntansha) HATAKEYAMA Tomomitsu  Nagasaki University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (50228467)
ISHIMATSU Atsushi  Nagasaki University, Faculty of Fisheries, Professor, 水産学部, 教授 (00184565)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsRed Tide / Plankton / Reactive Oxygen Species / Toxicity / Chattonella / Raphyidophyceae / Lectin
Research Abstract

Chattonella marina, a raphidophycean flagellate, is one of the most noxious red tide phytoplankton and is highly toxic to fish, especially to yellowtail Seriola quinqueradiata. Although the precise mechanism of the toxic action of Chattonella sp. remains unclear, it has been demonstrated that a decrease in oxygen partial pressure of arterial blood is the earliest physiological disturbance observed in fish after exposure to C. marina, Physiological and histological studies of fish exposed to C. marina also suggested that the blockade of respiratory water flow through the gill lamellae caused by excessive mucus interferes with O2 transfer, resulting in asphyxia. Recent studies have shown that Chattonella spp. Generate reactive oxygen species (ROS) such as O2-, H2O2, and hydroxyl radical (・OH). Furthermore, it has been reported that Heterosigma akashiwo, which is also classified in a raphidophycean, produces ROS, and showed an oxygen-radical-mediated toxic effect on rainbow trout, Oncorhy … More nchus mykiss. Thus, it is likely that the production of ROS is a common feature of raphidophycean flagellates. In the present study, we found that lectins such as concanavalin A (Con A), wheat germ agglutinin (WGA), and castor bean hemagglutinin (CBH) stimulated C. marina and H. akashiwo to generate enhanced amounts of O2- in a concentration dependent manner. The lectin-specific sugars potently inhibited the lectin-induced increase of O2-production, suggesting that the effects of lectins are mediated mainly through the interaction of these lectins with carbohydrate moiety present on the flagellate cell surface. In contrast to the potent ability of native Con A (tetravalent), succinylated Con A (divalent) showed only slight stimulative effect on these flagellates. O2- production was totally inhibited by treatment with proteinase K for 30 min, without affecting the viabilities of flagellates. These results suggest that cell-surface redox enzyme may be involved in O2- production, and such enzyme is responsible for the lectin-stimulation. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] 石松惇、小田達也: "シャットネラの活性酸素産生と魚類へい死"海洋. 30・3. 175-180 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. oda, N. Komatsu, and T. Muramatsu: "Diisopropylfluorophosphate (DFP) inhibits rich-induced apoptosis of MDCK cells"Biosci. Biotechnol. Biochem.. 62・2. 325-333 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Matsubara, R. Kawada, K. Iwasaki, T. Oda, and T. Muramatsu: "Extracellular poly(a-L-guluronate)lyase from Corynebacterium sp/: Purification, characteristics, and conformational properties"J. Protein Chemistry. 17・1. 29-36 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Fukuda, N. hamaguchi, T.Oda, A. Ishimatsu, and T. Muramatsu: "Hemagglutinating activity of extracellular alkaline metalloendopeptidases from Vibrio sp. NUF-BPP1"Biosci. Biotechnol. Biochem.. 62・6. 1157-1160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, A. Nakamura, T. Okamoto, A. Ishimatsu, and T. Muramatsu: "Lectin-induced enhancement of superoxide anion production by red tide phytoplankton"Mar. Biol.. 131. 383-390 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Komatsu, T. Oda, and T. Muramatsu: "Involvement of both caspase-like proteases and serine proteases in apoptotic cell death induced by ricin, modeccin, diphtheria toxin, and Pseudomonas toxin"J. Biochem.. 124. 1038-1044 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A. Nakamura, T. Okamoto, N. Komatsu, S. Ooka, T. Oda, A. Ishimatsu, and T. Muramatsu: "Fish mucus stimurates the generation of superoxide anion by Chattonella marina and Heterosigma akashiwo"Fisheries Science. 64・6. 866-869 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Hishida, H. Katoh, T. Oda, and A. Ishimatsu: "Comparison of physiological response to exposure to Chattonella marina in yellowtail, red sea bream and Japanese flounder"Fisheries Science. 64・6. 875-881 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Hishida, A. Ishimatsu and T. Oda: "Effect of environmental hyperoxia on respiration of yellowtail exposed to Chattonella marina."Fisheries Science. 65・1. 84-90 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, N. Shinmura, Y. Nishioka, N. Komatsu, T. Hatakeyama, and T. Muramatsu: "Effect of the hemolytic lectin CEL-III from holothuroidea Cucumaria echinata on the ANS fluorescence responses in sensitive MDCK and resistant CHO cells"J. Biochem.. 125. 713-720 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, J.Iwaoka, N. Komatsu, and T. Muramatsu: "Involvement of N-acetylcysteine-sensitive path ways in ricin-induced apoptotic cell death in U937 cells"Biosci. Biotechnol. Biochem.. 63. 341-348 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, N. Sadakata, N. Komatsu, and T. Muramatsu: "Specific efflux of glutathione from the basolateral membrane domain in polarized MDCK cells during ricin-induced apoptosis"J. Biochem.. 126. 715-721 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D. Kim, A. Nakamura, T. Okamoto, N. Komatsu, T.Oda, A. Ishimatsu, and T. Muramatsu: "Toxic potential of the raphidophyte Olisthodiscus luteus: mediation by reactive oxygen species"J. Plankton Res.. 21・6. 1017-1027 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D. Kim, T. Oda, A. Ishimatsu, and T. Muramatsu: "Isolation and characterization of a mutant strain of Chattonella marina with decreased production of superoxide anion"Biosci. Biotechnol. Biochem.. 63・11. 1947-1952 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D. Kim, T. Oda, A. Ishimatsu, and T. Muramatsu: "galacturonic-acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo"Biosci. Biotechnol. Biochem.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, N. Komatsu, and T. Muramatsu: "Diisopropylfluorophosphate (DFP) inhibits ricin-induced apoptosis of MDCK cells"Biosci. Biotechnol. Biochem.. 62-2. 325-333 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Matsubara, R. Kawada, K. Iwasaki, T. Oda, and T. Muramatsu: "Extracellular poly (a-L-guluronate) lyase from Corynebacterium sp.: Purification, characteristics, and conformational properties"J. Protein Chem.. 17-1. 29-36 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Fukuda, N. Hamaguchi, T. Oda, A. Ishimatsu, and T. Muramatsu: "Hemagglutinating activity of extracellular alkaline metalloendopeptidases from Vibrio sp. NUF-BPP1"Biosci. Biotechnol. Biochem.. 62-6. 1157-1160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, A. Nakamura, T. Okamoto, A. Ishimatsu, and T. Muramatsu: "Lectin-induced enhancement of superoxide anion production by red tide phytoplankton"Mar. Biol.. 131. 383-390 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Komatsu, T. Oda, and T. Muramatsu: "Involvement of both caspase-like proteases and serine proteases in apoptotic cell death induced by ricin, modeccin, diphtheria toxin, and Pseudomonas toxin"J. Biochem.. 124. 1038-1044 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A. Nakamura, T. Okamoto, N. Komatsu, S. Ooka, T. Oda, A. Ishimatsu, and T. Muramatsu: "Fish mucus stimurates the generation of superoxide anion by Chattonella marina and Heterosigma akashiwo."Fisheries Science. 64-6. 866-869 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Hishida H. Katoh, T. Oda, and A. Ishimatsu: "Comparison of physiological response to exposure to Chattonella marina in yellowtail, red sea bream and Japanese flounder"Fisheries Science. 64-6. 875-881 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Hishida, A. Ishimatsu, and T. Oda: "Effect of environmental hyperoxia on respiration of yellowtail exposed to Chattonella marina"Fisheries Science. 65-1. 84-90 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, N. Shinmura, Y. Nishioka, N. Komatsu, T. Hatakeyama, and T. Muramatsu: "Effect of the hemolytic lectin CEL-III from holothuroidea Cucumaria echinata on the ANS fluorescence responses in sensitive MDCK and resistant CHO cells"J. Biochem.. 125. 713-720 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, J. Iwaoka, N. Komatsu, and T. Muramatsu: "Involvement of N-acetylcysteine-sensitive pathways in ricin-induced apoptotic cell death in U937 cells"Biosci. Biotechnol. Biochem.. 63-2. 341-348 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Oda, N. Sadakata, N. Komatsu, and T. Muramatsu: "Specific efflux of glutathione from the basolateral membrane domain in polarized MDCK cells during ricin-induced apoptosis"J. Biochem.. 126. 715-721 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D. Kim, A. Nakamura, T. Okamoto, N. Komatsu, T. Oda, A. Ishimatsu, and T. Muramatsu: "Toxic potential of the raphidophyte Olisthodiscus luteus: mediation by reactive oxygen species."J. Plankton Res.. 21-6. 1017-1027 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D. Kim, T. Oda, A. Ishimatsu, and T. Muramatsu: "Isolation and characterization of a mutant strain of Chattonella marina with decreased production of superoxide anion"Biosci. Biotechnol. Biochem.. 63-11. 1947-1952 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D. Kim, T. Oda, A. Ishimatsu, and T. Muramatsu: "Galacturonic-acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo"Biosci. Biotechnol. Biochem.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 石松惇,小田達也: "シャットネラの活性酸素産生と魚類へい死"海洋. 30・3. 175-180 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Oda,N.Komatsu,and T.Muramatsu: "Diisopropylfluorophosphate (DFP) inhibits ricin-induced apoptosis of MDCK cells"Biosci.Biotechnol.Biochem.. 62・2. 325-333 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Matsubara,R.Kawada,K.Iwasaki,T.Oda,and T.Muramatsu: "Extracellular poly (a-L-guluronate) Iyase from Corynebacterium sp.: Purification characteristics,and conformational properties"J.Protein Chemistry. 17・1. 29-36 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Fukuda,N.Hamaguchi,,T.Oda,A.Ishimatsu,and T.Muramatsu: "Hemagglutin ating activity of extracellular alkaline metalloendopeptidases from Vibrio sp.NUF-BPP1"Biosci.Biotechnol.Biochem.. 62・6. 1157-1160 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Oda,A.Nakamura,T.Okamoto,A.Ishimatsu,and T.Muramatsu: "Lectin -induced en hancement of superoxide anion production by red tide phytoplankton"Mar.Biol.. 131. 383-390 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] N.Komatsu,T.Oda,and T.Muramatsu: "Involvement of both caspase-like proteases and serine proteases in apoptotic cell death induced by ricin,modeccin,diphtheria toxin,and Pseudomonas toxin"J.Biochem.. 124. 1038-1044 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] A.Nakamura,T.Okamoto,N.Komatsu,S.Ooka,T.Oda,A.Ishimatsu,and T.Muramatsu: "Fish mucus stimurates the generation of superoxide anion by Chattonella marina and Heterosigma akashiwo."Fisheries Science. 64・6. 866-869 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Hishida H.Katoh,T.Oda,and A.Ishimatsu: "Comparison of physiological response to exposure to Chattonella marina in yellowtail,red sea bream and Japanese flounder"Fisheries Science. 64・6. 875-881 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Hishida,A.Ishimatsu,and T.Oda: "Effect of environmental hyperoxia on respiration of yellowtail exposed to Chattonella marina"Fisheries Science. 65・1. 84-90 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] D.Kim,A.Nakamura,T.Okamoto,N.Komatsu,T.Tatsuya,A.Ishimatsu,and T.Muramatsu: "Toxic potential of the raphidophyte Olisthodiscusluteus: mediation by reactive oxygen species"J.Plankton Res.. 21・6. 1017-1027 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] D.Kim,T.Oda,A.Ishimatsu,and T.Muramatsu: "Efsolation and characterization of a mutant of Chattonellla with decreased production of superoxide anion"Biosci.Biotechnol.Biochem.. 63・11. 1947-1952 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 石松 惇,小田達也: "シャットネラの活性酸素産生と魚類へい死" 海洋. 30・3. 175-180 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Oda,N.Komatsu,and T.Muramatsu: "Diisopropylfluorophosphate(DFP)inhibits ricin-induced apoptosis of MDCK cells" Biosci.Biotech.Biochem.62・2. 325-333 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Matsubara,R.Kawada,K.Iwasaki,T.Oda,and T.Muramatsu: "Extracellular poly(a-L-guluronate)lyase from Corynebacterium sp.:Purification,characteristics,and conformational properties" J.Protein Chemistry. 17・1. 29-36 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Fukuda,N.Hamaguchi,,T.Oda,A.Ishimatsu,and T.Muramatsu: "Hemagglutinating activity of extracellular alkaline metalloendopeptidases from Vibrio sp.NUF-BPP1" Biosci.Biotech.Biochem.62・6. 1157-1160 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Oda,A.Nakamura,T.Okamoto,A.Ishimatsu,and T.Muramatsu: "Lectin-induced enhancement of superoxide anion production by red tide phytoplankton" Mar.Biol.131. 383-390 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Komatsu,T.Oda,and T.Muramatsu: "Involvement of both caspase-like proteases and serine proteases in apoptotic cell death induced by ricin,modeccin,diphtheria toxin,and Pseudomonas toxin" J.Biochem.124. 1038-1044 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Nakamura,T.Okamoto,N.Komatsu,S.Ooka,T.Oda,A.Ishimatsu,and T.Muramatsu: "Fish mucus stimurates the generation of superoxide anion by Chattonella marina and Heterosigma akashiwo." Fisheries Science. 64・6. 866-869 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Hishida,H.Katoh,T.Oda,and A.Ishimatsu: "Comparison of physiological response to exposure to Chattonella marina in yellowtail,red sea bream and Japanese flounder" Fisheries Science. 64・6. 875-881 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Hishida,A.Ishimatsu,and T.Oda: "Effect of environmental hyperoxia on respiration of yellowtail exposed to Chattonella marina." Fisheries Science (in press). 65. (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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