| Project/Area Number |
10660191
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General fisheries
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
EGUCHI Mitsuru Kinki University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (40176764)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yuji Tokyo University of Fisheries, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (90207150)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | ayu / vibriosis / Vibrio anguillarum / amphipod / Jesogammarus annandalei / culturability / fluorescent antibody technique / VBNC / Vibro anguillarum / Jesogammarus annandalei / モノクローナル抗体 |
|---|
| Research Abstract |
Vibriosis caused by Vibrio anguillarum seriously damages freshwater fish (Salmoniforms) almost every year in Lake Biwa, Japan. This pathogen requires NaCl for its growth. When the pathogen was exposed to sterilized aged lake water, it lost its culturability without loosing respiratory activity. Although this physiological state looks like "VBNC", they could not resuscitate even in host fish bodies. As these cells totally lost their pathogenicity, the VBNC-like state is probably on the way to death. Environmental factors such as darkness, coldness and anaerobiosis helped the pathogen to survive in freshwater without getting into nonculturable state. Culturable V. anguillarum cells were detected from planktonic samples such as freshwater amphipods (Jesogammarus annandalei), which migrate vertically. This indicates that freshwater amphipods have roles as intermediate hosts in the infectious process of V. anguillarum, which were deposited on the bottom sediments, to the final host, ayu.
|
