| Project/Area Number |
10660194
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
|
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MATSUI Takashi Grad. School Agr. Lifesci., The University of Tokyo, Lecturer, 大学院・農学生命科学研究科, 講師 (90011981)
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|---|
| Project Period (FY) |
1998 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
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| Keywords | tetrodotoxin / puffer fish toxin / kusafugu / TTX binding protein / toxin accumulation |
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| Research Abstract |
A tetrodotoxin binding protein has been purified from the plasma of the puffer fish ku safugu, Takifugu niphobles, through DEAE-cellulose treatment, ammonium sulfate fractionation, Sephadex gelfiltrations, and Sephacryl S-200 and Cellulofine A-500 column chromatography. Final purification by HPLC on a TSK G-3000 SL column yielded a protein which showed only a single protein peak . The molecular weight of the protein was estimated to be 116,000 and 96,000 by SDS-PAGE and mass spectrometry, respectively. A blast search on the amino-terminal amino acid sequence of thc purified protein revealed that thc protein had no homology to any other protein on data base.
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