Studies on molecular mechanisms and artificial regulation of maturation and aging of mammalian oocytes
| Project/Area Number |
10660267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAITO Kunihiko University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20188858)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | MPF / oocyte aging / fragmentation / caffeine / vanadate / matured porcine oocyte / 豚卵子 / 老化 / p34^<cde2> / サイクリンB |
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| Research Abstract |
Aims of the present study are clarifying the cytoplasmic changes during mammalian oocyte aging at molecular levels and subsequently regulating this process artificially. The present study might contribute to prevent the deterioration of oocyte qualities derived from elongated manipulation period of the in vitro matured mammalian oocytes, used for such as reproductive and developmental technologies.
The gradual decrease of maturation promoting factor (MPF) activity during oocyte aging has been reported previously. The present study revealed that the molecular mechanism of the decrease of MPF activity during oocyte aging was completely different from that during oocyte activation Although the decrease of MPF activity at oocyte activation was attributed to the rapid degradation of cyclin B, a regulatory subunit of MPF, the levels of MPF subunits, both p34ィイD1cdc2ィエD1 and cyclin B, were not significant]y changed during oocyte aging but the gradual accumulation of hyperphosphorylated inactiv
… More
e MPF, so- called pre-MPF, was observed. In order to confirm that this hyperphosphorylation of MPF was the course of the decreased MPF activity in aged oocytes, I treated the fresh and aged oocytes, respectively, with vanadate and caffeine which modulated the phosphorylation states of MPF. These experiments suggested that the change of phosphorylation state of MPF was the main course of the decrease of the activity during oocyte aging and that the MPF activity in aged oocytes could be regulated at lease in part by vanadate and caffeine treatment. These findings might be valuable as the first report showing the possibility of artificial regulation of MPF activity. Furthermore, I revealed that these treatments could change the rates of spontaneous activation and fragmentation of oocytes, both are the parameters of oocyte aging.
In summary, the present study showed cytoplasmic changes during mammalian oocyte aging at molecular levels and proposed a simple method for artificial regulation of this process at least partially. Less
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Report
(3 results)
Research Products
(14 results)
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[Publications] KIKUCHI, K., NAITO, K., NOGUCHI, J., SHIMADA, A., KANEKO, H., YAMASHITA, M., TOJO, H. and TOYODA Y.: "Inactivation of p34ィイD1cdc2ィエD1 kinase by the accumulation of its phosphorylated forms in porcine oocytes matured and aged in vitro."Zygote. 7. 173-179 (1999)
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[Publications] SUGIURA, K., NAITO, K,. KAGII, H., IWAMORI, N., YAMANOUCHI, K. and TOJO, H.: "Analysis of the germinal vesicle requirement for the activation of MPF in maturation of porcine oocytes."J. Mamm. Ova Res.. 16. 130-134 (1999)
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[Publications] NAITO, K., KAGII, H., IWAMORl, N., SUGIURA, K., YAMANOUCHI, K. and TOJO, H.: "Establishment of a small-scale western blotting system named as "micro-western blotting" for mammalian ova analysis."J. Mamm. Ova Res.. 16. 154-157 (1999)
Description
「研究成果報告書概要(欧文)」より
Related Report
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