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The role of MAP kinase cascade in activation of porcine oocytes

Research Project

Project/Area Number 10660271
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionHiroshima University

Principal Investigator

TERADA Takato  Hiroshima University, Faculty of Applied Biological Science, Professor, 生物生産学部, 教授 (60034477)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordspig / oocyte / MAP kinase / activation / cumulus cell / gap junction / PI 3-kinase 8 MPF / MPF / PI3-kinase / ブタ卵子 / MEK inhibitor / 卵子の活性化 / コネキシン
Research Abstract

The role of MAP kinase during maturation of porcine oocytes and the activation mechanisms were explored. MAP kinase in the oocytes was dramatically activated in metaphase I and the activated MAP kinase led to activation of MPF. These results suggest that the activation of MAP kinase is essential for the normal progression into the metaphase of the second meiosis. Further, the evidences that MAP kinase activation in oocytes were completely dependent on the activity of PI 3-kinase in the surrounding cumulus cells, inducing a shutdown of the gap junctions between oocytes and cumulus cells, were provided. Next, zona reaction which is an indicator of CaィイD12+ィエD1 transients at oocyte activation was assessed. The procedure for detecting a modification of glycoproteins in zona pellucida of a single oocyte, was devised. Using this procedure, it was demonstrated that the MPF activity declined quickly in oocytes activated by CaィイD12+ィエD1 ionophore at low concentration, but the activity of MAP ki … More nase remained high, resulting in reactivation of MPF and the oocyte progression into the MIII stage, which is considered to be the abnormal event. Moreover, the combined treatment with CaィイD12+ィエD1 ionophore at low concentration and specific MEK inhibitor, U0126, resulted in dramatic drop in activities of MPF and MAP kinase, and subsequently activities of both kinase was maintained at the basal level until the development of a pronucleus was induced in the oocytes. These findings clearly demonstrate that maintaining the reduced activity of MPF due to the decline of MAP kinase activity is critical for completion of meiotic maturation and the formation of pronuclei in porcine oocytes. Taken together, the following conclusions can be drown from this study ; 1) The activity of MAP kinase is porcine oocytes is regulated by the surrounding cumulus cells. 2) The activation of MAP kinase is a prerequisite for the progression beyond metaphase I into metaphase II. 3) MAP kinase is inactivated by elevation of CaィイD12+ィエD1 in MII arrested oocytes, which is closely associated with completion of meiosis and formation of pronuclei. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Shimada M.,Idris M.A. Terada T: "Effects of Phosphatidylinositol 3-Kinase lnhibilors,Wortmannin and LY294002 on Germlinal Vesicle Breakdown(GVBD)in Porcine Oocytes"J.Reprod.Dev. 44. 281-288 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Isobe N,Maeda T,and Terada T: "Involvement of meiolic resumption in the disruption of gap junctions between cumulus cells attached to pig oocytes"J.Reprod.Fertil. 113. 167-172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimada M.,Idris M.A. Terada T: "Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic progression from M I to M II stage in Porcine Follicular Oocvtes"J.Mamm.Ova Res. 15. 68-76 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Shimada and T.Terada: "Phosphorylation of connexin-43,gap junctional protein,in cumulus cells is regulated by mitogen-activated protein kinase and phosphatidylinositol 3-kinase during in vitro meiotic rcsumption in porcine follictllar oocyles"J.Mamm.Ova Res. 16. 37-42 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Tatemoto and T.Terada: "Analysis of zona pellucida modification due to cortical granule exocytosis in single porcine oocytes,using enhanced chemiluminescence"Theriogenology. 52. 629-640 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Tatemoto and T.Terada: "Time course analysis of cortical granule exocytosis and zona pellucida modifications after artificial activation in in-vitro matured porcine oocytes"J.Mamm.Ova Res. 16. 110-117 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimada M., Idris M.A. and Terada T.: "Effects of Phosphatidylinositol 3-Kinase Inhibitors, Wortmannin and LY294002 on Germinal Vesicle Breakdown (GVBD) in Porcine Oocytes."J. Reprod. Dev.. vol. 44. 281-288 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Isobe N, Maeda T, and Terada T.: "Involvement of meiotic resumption in the disruption of gap junctions between cumulus cells attached to pig oocytes."J. Reprod. Fertil.. vol. 113. 167-172 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimada M., Idris M.A. and Terada T.: "Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from M I to M II Stage in Porcine Follicular Oocytes."J. Mamm. Ova Res.. Vol. 15. 68-76 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimada M. and Terada T.: "Phosphorylation of Connexin-43, Gap Junctional Protein, in Cumulus Cells Are Regulated by Mitogen Activated Protein Kinase and Phosphatidylinositol 3-Kinase during In Vitro Meiotic Resumption in Porcine Follicular Oocytes."J. Mamm. Ova Res. Vol. 16. 37-42 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tatemoto, H and Terada T.: "Analysis of zona pellucida modifications due to cortical granule exocytosis in single porcine oocytes, using enhanced chemiluminescence."Theriogenology. vol. 52. 629-640 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tatemoto, H and Terada, T.: "Time course analyses of cortical granule exositosis and zona pellucida modifications after artificial activation in in vitro matured porcine oocytes"J. Mamm. Ova Res. vol. 16. 110-117 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M.Shimada and T.Terda: "Phosphorylation of connexin-43,gap junctional protein,in cumulus cells is regulated by mitogen-actibated protein kinase and phosphatisylinositol 3-kinase during in vitro meiotic resumption in porcine follicular oocytes"J.Mamm.Ova Res.. 16(1). 37-42 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Tatemoto and T.Terda: "Analysis of zona pellucida modification due to coetical granule exocytosis in single porcine oocytes,using enhanced chemiluminescence"Theriogenology. 52(4). 629-640 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Tatemoto and T.Terda: "Time course analysis of contical granule exocytosis and pellucida modification after artificial activation in in-vitro matured porcine oocytes"J.Mamm.Ova Res.. 16(3). 110-117 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Shimada: "Effects of phosphatidylinositol 3-kinase inhibitors, wortmannin and LY294002, on germinal vesicle breakdown (GVBD) in porcine cocytes" Journal of Reproduction and Development. 44(3). 281-288 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Shimada: "Phosphatidylinositol 3-kinase in cumulus cells is responsible for meictic progession from MI to MII stage in porcine follicular oocytes" J Mamm.Ova Res. 15(1). 68-76 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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